scholarly journals Experimental characterization of 3D localization techniques for particle-tracking and super-resolution microscopy

2009 ◽  
Vol 17 (10) ◽  
pp. 8264 ◽  
Author(s):  
Michael J. Mlodzianoski ◽  
Manuel F. Juette ◽  
Glen L. Beane ◽  
Joerg Bewersdorf
Keyword(s):  
Particle Tracking ◽  
Super Resolution ◽  
Experimental Characterization ◽  
3D Localization ◽  
Super Resolution Microscopy

scholarly journals Characterization of Plasma Membrane Ceramides by Super-Resolution Microscopy

2017 ◽  
Vol 56 (22) ◽  
pp. 6131-6135 ◽  
Author(s):  
Anne Burgert ◽  
Jan Schlegel ◽  
Jérôme Bécam ◽  
Sören Doose ◽  
Erhard Bieberich ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals Can Super-Resolution Microscopy Become a Standard Characterization Technique for Material Chemistry?

2021 ◽  
Author(s):  
Shikha Dhiman ◽  
Teodora Andrian ◽  
Beatriz Santiago ◽  
Marrit Tholen ◽  
Yuyang Wang ◽  
...  
Keyword(s):  
Super Resolution ◽  
Analytical Techniques ◽  
Material Chemistry ◽  
Characterization Technique ◽  
Super Resolution Microscopy ◽  
Synthesized Materials

The characterization of newly synthesized materials is a cornerstone of all chemistry and nanotechnology laboratories. For this purpose, a wide array of analytical techniques have been standardized and are used...

scholarly journals Photoactivation of silicon rhodamines via a light-induced protonation

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Michelle S. Frei ◽  
Philipp Hoess ◽  
Marko Lampe ◽  
Bianca Nijmeijer ◽  
Moritz Kueblbeck ◽  
...  
Keyword(s):  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Spectroscopic Properties ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Localization Microscopy ◽  
Super Resolution Microscopy ◽  
Single Molecule Localization Microscopy

Abstract Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

Experimental characterization of axial fuel mixing in fluidized beds by magnetic particle tracking

2017 ◽  
Vol 316 ◽  
pp. 492-499 ◽  
Author(s):  
Anna Köhler ◽  
Alexander Rasch ◽  
David Pallarès ◽  
Filip Johnsson
Keyword(s):  
Particle Tracking ◽  
Fluidized Beds ◽  
Magnetic Particle ◽  
Experimental Characterization ◽  
Fuel Mixing

scholarly journals Characterization of Ire1 Interactions and Dynamics with Quantitative Super-Resolution Microscopy

2017 ◽  
Vol 112 (3) ◽  
pp. 148a
Author(s):  
Elizabeth M. Smith ◽  
Ragnar Stefansson ◽  
Maria Paz Ramirez Lopez ◽  
Elias M. Puchner
Keyword(s):  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals Combining 3D single molecule localization strategies for reproducible bioimaging

2018 ◽  
Author(s):  
Clément Cabriel ◽  
Nicolas Bourg ◽  
Pierre Jouchet ◽  
Guillaume Dupuis ◽  
Christophe Leterrier ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
3D Localization ◽  
Point Spread ◽  
Spread Function ◽  
Multicolor Imaging ◽  
Localization Precision ◽  
Different Depths ◽  
Super Resolution Microscopy ◽  
Living Bacteria

We developed a 3D localization-based super-resolution technique providing a slowly varying localization precision over a 1 μm range with precisions down to 15 nm. The axial localization is performed through a combination of point spread function (PSF) shaping and supercritical angle fluorescence (SAF), which yields absolute axial information. Using a dual-view scheme, the axial detection is decoupled from the lateral detection and optimized independently to provide a weakly anisotropic 3D resolution over the imaging range. This method can be readily implemented on most homemade PSF shaping setups and provides drift-free, tilt-insensitive and achromatic results. Its insensitivity to these unavoidable experimental biases is especially adapted for multicolor 3D super-resolution microscopy, as we demonstrate by imaging cell cytoskeleton, living bacteria membranes and axon periodic submembrane scaffolds. We further illustrate the interest of the technique for biological multicolor imaging over a several-μm range by direct merging of multiple acquisitions at different depths.

scholarly journals Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design

2021 ◽  
Vol 9 ◽  
Author(s):  
Maria Arista-Romero ◽  
Silvia Pujals ◽  
Lorenzo Albertazzi
Keyword(s):  
Viral Infection ◽  
Fluorescent Dyes ◽  
Super Resolution ◽  
Particle Characterization ◽  
Emerging Viruses ◽  
Potential Threat ◽  
The Right ◽  
Super Resolution Microscopy ◽  
Selection Of

In the last year the COVID19 pandemic clearly illustrated the potential threat that viruses pose to our society. The characterization of viral structures and the identification of key proteins involved in each step of the cycle of infection are crucial to develop treatments. However, the small size of viruses, invisible under conventional fluorescence microscopy, make it difficult to study the organization of protein clusters within the viral particle. The applications of super-resolution microscopy have skyrocketed in the last years, converting this group into one of the leading techniques to characterize viruses and study the viral infection in cells, breaking the diffraction limit by achieving resolutions up to 10 nm using conventional probes such as fluorescent dyes and proteins. There are several super-resolution methods available and the selection of the right one it is crucial to study in detail all the steps involved in the viral infection, quantifying and creating models of infection for relevant viruses such as HIV-1, Influenza, herpesvirus or SARS-CoV-1. Here we review the use of super-resolution microscopy (SRM) to study all steps involved in the viral infection and antiviral design. In light of the threat of new viruses, these studies could inspire future assays to unveil the viral mechanism of emerging viruses and further develop successful antivirals against them.

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