Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

10.3791/62754 ◽  
2021 ◽  
Author(s):  
Yue Zhang ◽  
Peng Cao ◽  
Fang Lu ◽  
Jinjun Cheng ◽  
Huihua Qu
Keyword(s):  
Small Molecule ◽  
Lateral Flow ◽  
Quantitative Detection ◽  
Immunochromatographic Strip

scholarly journals Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

RSC Advances ◽  
2018 ◽  
Vol 8 (17) ◽  
pp. 9580-9586 ◽  
Author(s):  
Yaning Sun ◽  
Jifei Yang ◽  
Suzhen Yang ◽  
Qingbo Sang ◽  
Man Teng ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Quantitative Detection ◽  
Lateral Flow Strip ◽  
Immunochromatographic Strip ◽  
Gentamicin Sulfate ◽  
Kanamycin Sulfate ◽  
Neomycin Sulfate

A colloidal gold-based immunochromatographic strip has been developed for the rapid, simultaneous, semi-quantitative and quantitative detection of several aminoglycoside residues in milk, including gentamicin sulfate (GM), neomycin sulfate (NEO) and kanamycin sulfate (KN).

scholarly journals A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

2021 ◽  
Vol 18 (9) ◽  
pp. 4574
Author(s):  
Kai Chen ◽  
Biao Ma ◽  
Jiali Li ◽  
Erjing Chen ◽  
Ying Xu ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Quantitative Detection ◽  
Bacterial Strains ◽  
Food Borne Pathogens ◽  
Food Borne

Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Europium nanoparticles (EuNPs) are used as fluorescent probes in lateral flow immunoassays (LFIAs) to improve sensitivity. Here, recombinase polymerase amplification (RPA) combined with fluorescent LFIA was established for the simultaneous and quantitative detection of Listeria monocytogenes, Vibrio parahaemolyticus, and Escherichia coliO157:H7. In this work, the entire experimental process could be completed in 20 min at 37 °C. The limits of detection (LODs) of EuNP-based LFIA–RPA were 9.0 colony-forming units (CFU)/mL for Listeria monocytogenes, 7.0 CFU/mL for Vibrio parahaemolyticus, and 4.0 CFU/mL for Escherichia coliO157:H7. No cross-reaction could be observed in 22 bacterial strains. The fluorescent LFIA–RPA assay exhibits high sensitivity and good specificity. Moreover, the average recovery of the three food-borne pathogens spiked in food samples was 90.9–114.2%. The experiments indicate the accuracy and reliability of the multiple fluorescent test strips. Our developed EuNP-based LFIA–RPA assay is a promising analytical tool for the rapid and simultaneous detection of multiple low concentrations of food-borne pathogens.

TEMPO-based immuno-lateral flow quantitative detection of dengue NS1 protein

2018 ◽  
Vol 259 ◽  
pp. 354-363 ◽  
Author(s):  
Prima Dewi Sinawang ◽  
Luka Fajs ◽  
Kamal Elouarzaki ◽  
Jusak Nugraha ◽  
Robert S. Marks
Keyword(s):  
Lateral Flow ◽  
Quantitative Detection ◽  
Ns1 Protein

SERS-Based Lateral Flow Immunoassay Strip for Ultrasensitive and Quantitative Detection of Acrosomal Protein SP10

2021 ◽  
Author(s):  
Bing Liu ◽  
Shiya Zheng ◽  
Qian Liu ◽  
Bingbing Gao ◽  
Xiangwei Zhao ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection

Fluorescence-Raman dual-mode quantitative detection and imaging of small-molecule thiols in cell apoptosis with DNA-modified gold nanoflowers

2022 ◽  
Author(s):  
Chenbiao Li ◽  
Peifang Chen ◽  
Imran Mahmood Khan ◽  
Zhouping Wang ◽  
Yin Zhang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Cell Apoptosis ◽  
Quantitative Detection ◽  
Dual Mode ◽  
Physiological Processes ◽  
Gold Nanoflowers ◽  
Living Organisms

The monitoring of small-molecule thiols (especially glutathione) has attracted widespread attention due to their involvement in numerous physiological processes in living organisms and cells. In this work, the dual-mode nanosensor...

scholarly journals A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour

The Analyst ◽  
2015 ◽  
Vol 140 (1) ◽  
pp. 358-365 ◽  
Author(s):  
Martina Zangheri ◽  
Fabio Di Nardo ◽  
Laura Anfossi ◽  
Cristina Giovannoli ◽  
Claudio Baggiani ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Type B ◽  
Maize Flour

A multiplex chemiluminescence biosensor based on a lateral flow immunoassay was developed for on-site quantitative detection of fumonisins and aflatoxin B1 in maize.

Sign in / Sign up

Export Citation Format

Share Document