Determination of Total Lipid and Lipid Classes in Marine Samples

10.3791/62315 ◽  
2021 ◽  
Author(s):  
C. C. Parrish ◽  
J. S. Wells
Keyword(s):  
Total Lipid ◽  
Lipid Classes ◽  
Marine Samples

scholarly journals A Toolbox for the Determination of Nitroaromatic Explosives in Marine Water, Sediment, and Biota Samples on Femtogram Levels by GC-MS/MS

Toxics ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 60
Author(s):  
Tobias Hartwig Bünning ◽  
Jennifer Susanne Strehse ◽  
Ann Christin Hollmann ◽  
Tom Bötticher ◽  
Edmund Maser
Keyword(s):  
Sample Preparation ◽  
Freeze Drying ◽  
Solid Phase ◽  
Phase Extraction ◽  
Direct Extraction ◽  
Time Saving ◽  
Marine Samples ◽  
Volume Method ◽  
Biota Samples

To determine the amount of the explosives 1,3-dinitrobenzene, 2,4-dinitrotoluene, 2,4,6-trinitrotoluene, and its metabolites in marine samples, a toolbox of methods was developed to enhance sample preparation and analysis of various types of marine samples, such as water, sediment, and different kinds of biota. To achieve this, established methods were adapted, improved, and combined. As a result, if explosive concentrations in sediment or mussel samples are greater than 10 ng per g, direct extraction allows for time-saving sample preparation; if concentrations are below 10 ng per g, techniques such as freeze-drying, ultrasonic, and solid-phase extraction can help to detect even picogram amounts. Two different GC-MS/MS methods were developed to enable the detection of these explosives in femtogram per microliter. With a splitless injector, limits of detection (LODs) between 77 and 333 fg/µL could be achieved in only 6.25 min. With the 5 µL programmable temperature vaporization—large volume method (PTV-LVI), LODs between 8 and 47 fg/µL could be achieved in less than 7 min. The detection limits achieved by these methods are among the lowest published to date. Their reliability has been tested and confirmed by measuring large and diverse sample sets.

Rapid determination of 210 Pb and 210 Po in water and application to marine samples

Talanta ◽  
2016 ◽  
Vol 160 ◽  
pp. 28-35 ◽  
Author(s):  
M. Villa-Alfageme ◽  
J.L. Mas ◽  
S. Hurtado-Bermudez ◽  
P. Masqué
Keyword(s):  
Rapid Determination ◽  
Marine Samples

Determination of c9,t11-CLA in major human plasma lipid classes using a combination of methylating methodologies

Lipids ◽  
2003 ◽  
Vol 38 (7) ◽  
pp. 793-800 ◽  
Author(s):  
Alam M. Shahin ◽  
Michelle K. McGuire ◽  
Mark A. McGuire ◽  
Kristin L. Ritzenthaler ◽  
Terry D. Shultz
Keyword(s):  
Human Plasma ◽  
Plasma Lipid ◽  
Lipid Classes

scholarly journals Content and composition of lipid classes, fatty acid from \(\textit{Sargassum}\) seaweed collected at Con Dao and Van Phong bay

2021 ◽  
Vol 21 (3) ◽  
pp. 311-318
Author(s):  
Thu Hue Pham ◽  
Van Tuyen Anh Nguyen Nguyen ◽  
Yen Kieu Thi Hoang ◽  
Nguyen Nguyen ◽  
Hai Nam Hoang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Total Lipid ◽  
Polar Lipid ◽  
Brown Seaweed ◽  
Monounsaturated Fatty Acids ◽  
Lipid Classes ◽  
Average Content ◽  
Fresh Weight

This study studied the content and composition of the total lipid, lipid classes and fatty acids in 13 brown seaweed Sargassum species collected from Con Dao and Van Phong, Vietnam. The total lipid has a low content and varies among species from 0.10–1.70% of the fresh weight. From 13 species, seven lipid classes including polar lipid (Pol), free fatty acids (FFA), sterol (ST), hydrocarbon and wax (HW), triacylglycerol (TG), diacylglycerol (DG), and monoalkydiacylglycerol (MADG). Using the GC-FID technique, we have identified 29 fatty acids classified into 3 groups of saturated fatty acid, monounsaturated fatty acids, polyunsaturated fatty acids with an average content of 44.93%, 24.57% and 27.44%, respectively. Among those, many value fatty acids have been detected with high content such as C18:3n-3, C20:4n-6, 20:5n-3, and 22:6n-3. The lipid of 13 brown seaweed Sargassum species also fully contains omega-3,6,9 fatty acids with the content of 9.28%, 16.28% and 16.63%, respectively.

scholarly journals RESEARCH ON THE COMPONENT OF LIPID CLASSES, FATTY ACID, AMINO ACIDS FROM EGG AND BODY OF SEA URCHIN TRIPNEUSTES GRATILLA (LINNAEUS, 1758)

2018 ◽  
Vol 56 (4A) ◽  
pp. 30
Author(s):  
Kim Hoa Thi Dinh
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Sea Urchin ◽  
Total Lipid ◽  
Yellow Sea ◽  
Lipid Classes ◽  
The Yellow Sea ◽  
Viet Nam ◽  
Tripneustes Gratilla

ABSTRACT – HCTN 06The Sea urchin Tripneustes gratilla (Linnaeus, 1758) is a famous species of invertebrate member of the phylum Echinodermata of marine animals living in shallow tropical water. In Viet Nam, it is usually called by the name of yellow sea urchin. This species is mentioned much about its economic and commerce properties. Recently, many researchers have been paying attention to sea urchin because of its positive effect on human health. In terms of medicine and nutrition, the egg of yellow sea urchin was rich in Polyunsaturated Fatty Acids (PUFAs) and essential amino acids. In this research, we focused on determining the total lipid contents, the components of lipid classes and the compositions and contents of fatty acids, amino acids from the body and the egg of the yellow sea urchin T. gratilla (Linnaeus, 1758) collected in Hon Tam, Nha Trang, Khanh Hoa, Viet Nam in 2016. Although the yellow sea urchin T. gratilla has low percentage of total lipid in fresh weigh basis, they are full of essential lipid ingredients such as Wax and Hydrocabon (H + W), Triacylglycerol (TG), Monodiacylglycerol MDAG, Free Fatty Acid (FFA), sterol (ST), and Polar Lipid (PL). In both egg and body samples palmitic acid (16:0) was the most abundant fatty acid with the proportion of about 25%. In addition, arachidonic acid (20:4n-6) is dominent Omega-6 fatty acid in the Monounsaturated Fatty Acid group (MUFA). Furthermore, the research result also showed the very high percentages of omega-3 fatty acids in the egg and body of T. gratilla, with the total contents of 13.97% and 20.67% respectively, especially the presence of eicosapentaenoic fatty acid (C20:5n-3, EPA), a very valuable fatty acid. By the HPLC method, the research also found 17 amino acids in the egg and body of T. gratilla with the existence of 7 essential amino acids.Keywords: Tripneustes gratilla, lipid classes, yellow sea urchin, echinodermata, fatty acid content. 

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