scholarly journals Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

10.3791/61739 ◽  
2021 ◽  
Author(s):  
Mégane Bregeon ◽  
Nicolas Guyot ◽  
Sophie Réhault-Godbert
Keyword(s):  
Protein Solubilization ◽  
Mechanical Separation

Schistosoma mansoni: a comparative study of artificially transformed schistosomula and schistomula recoverd after cercarial penetration of isolated skin

Parasitology ◽  
1977 ◽  
Vol 74 (1) ◽  
pp. 73-86 ◽  
Author(s):  
Linda H. Brink ◽  
Diane J. McLaren ◽  
S. R. Smithers
Keyword(s):  
Comparative Study ◽  
Schistosoma Mansoni ◽  
Amorphous Material ◽  
Surface Membrane ◽  
Antigenic Determinants ◽  
Agglutination Reaction ◽  
Rat Serum ◽  
Mechanical Separation ◽  
B Blood Group

A comparison was made of the ultrastructure, development and antigenic nature of the surfaces and of the viability of three types of schistosomula of Schistosoma mansoni: schistosomula formed afrer cercariae had penetrated isolated skin (SS), schistosomula produced after mechanical separation of cercarial tails from bodies (MS), and schistosomula transformed from cercariae after incubation in fresh rat serum (RS).Within 2 h of transformation, the surface membrane of all three types of schistosomula had changed from trilaminate to heptalaminate structures and SS and MS had lost their cercarial glycocalyx. Initially a dense amorphous material was demonstrated on the surfaces of RS, which was thought to be the result of an interaction between a factor in rat serum and the glycocalyx: this material was greatly reduced within 2 h of transformation. The pre-acetabular glands of SS were emptied while those of MS and RS retained their contents. Immunofluorescent studies showed that all schistosomula bound serum from mice immune to S. mansoni, but the binding was stronger with MS and RS. The mixed agglutination reaction demonstrated the presence of human A and B blood group-like antigenic determinants on approximately 30% of 3 h old SS; these determinants were not detected on MS or RS. In vitro, the development of MS and RS was similar to SS; the first schistosomula reached the ‘gut-closed’ stage by day 10; 50–70% of SS reached this stage by day 12, in contrast to only 25–50% of MS and RS. Between 28 and 45% of all schistosomula developed to maturity when injected intravenously into mice.It was concluded that the two types of artificially prepared schistosomula fultil the main criteria of transformation from cercaria to schistosomulum. Further, it is suggested that MS are the most appropriate source of material for immunochemical and physiological studies.

Inadvertent stromal dissection during mechanical separation of the corneal epithelium using an epikeratome

2006 ◽  
Vol 32 (10) ◽  
pp. 1759-1763 ◽  
Author(s):  
Jun-Heon Kim ◽  
Chung-Hoon Oh ◽  
Jong-Suk Song ◽  
Hyo-Myung Kim
Keyword(s):  
Corneal Epithelium ◽  
Mechanical Separation

Considerations in the Design of the Primary Treatment for Ballast Systems

2003 ◽  
Vol 40 (01) ◽  
pp. 49-60
Author(s):  
Michael G. Parsons
Keyword(s):  
Water Treatment ◽  
Great Lakes ◽  
Ballast Water ◽  
Primary Treatment ◽  
Coast Guard ◽  
Secondary Treatment ◽  
Bulk Carrier ◽  
Ballast Water Treatment ◽  
Mechanical Separation ◽  
Hierarchy Process

Investigations are currently underway to establish effective primary and secondary ballast water treatment methods to minimize the potential for the introduction of additional nonindigenous aquatic species into the Great Lakes and other U.S. coastal waters. This treatment could be used in place of mid-ocean ballast exchange currently required by the U.S. Coast Guard for all vessels entering the Great Lakes in ballast from beyond the Exclusive Economic Zone (EEZ). Primary and secondary treatment could provide environmental protection for both Ballast On Board (BOB) vessels, which are required to perform mid-ocean ballast exchange before entering the Great Lakes, and No Ballast On Board (NOBOB) vessels, which are currently exempt from any ballast exchange requirements. Primary treatment using some form of mechanical separation to 100 urn or 50 um followed by secondary treatment using 254 nm UV irradiation or some form of chemical treatment are currently leading candidates. Over the past six years, the Great Lakes Ballast Technology Demonstration Project (GLBTDP) has undertaken the full-scale evaluation of 340 m3/h (1500 U.S. gpm) ballast water mechanical separation using an automatic backwashing screen filter, hydrocyclone, and automatic backwashing disk filter. This experience provides the basis for the investigation of various ballast system design issues that must be considered in the selection and design of the primary ballast water treatment. This investigation is based upon the ballast system of a typical Seaway size bulk carrier using port and starboard 2000 m3/h (8800 U.S. gpm) main ballast pumps. A discrete multicriterion optimization tradeoff study using the Analytical Hierarchy Process (AHP) is also presented to illustrate a rational method for determining the best choice for primary ballast water treatment for such a Seaway size bulk carrier.

scholarly journals Microscopy and protein solubilization of digesta from pigs fed wheat, corn, or soybean meal-based diets, with or without protease and a Bacillus spp. direct-fed microbial

2019 ◽  
Vol 247 ◽  
pp. 183-193 ◽  
Author(s):  
Laura Payling ◽  
Tofuko A. Woyengo ◽  
Mogens Nielsen ◽  
Hans H. Stein ◽  
Maria C. Walsh ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Protein Solubilization ◽  
Bacillus Spp

Digestate mechanical separation: Efficiency profiles based on anaerobic digestion feedstock and equipment choice

2019 ◽  
Vol 274 ◽  
pp. 180-189 ◽  
Author(s):  
F. Guilayn ◽  
J. Jimenez ◽  
M. Rouez ◽  
M. Crest ◽  
D. Patureau
Keyword(s):  
Anaerobic Digestion ◽  
Separation Efficiency ◽  
Mechanical Separation

scholarly journals An Experimental Analysis of Different Cane Juice Centrifugation in the Sugar Industries

2016 ◽  
pp. 01-06
Author(s):  
Prof. Vaishali Sarangpure
Keyword(s):  
Experimental Analysis ◽  
Full Scale ◽  
Feed Stream ◽  
Cane Juice ◽  
Mechanical Separation ◽  
High Consistency ◽  
Important Activity

Centrifugation is one of the key unit undertakings in the sugar business. Usually it is used ensuing to thickening of the stick juice, for instance at a high consistency region. The makers are of the view that a sedimentation hub will have the most sensible. The structure features of centrifugals from cluster to steady sorts have been thinks broadly, their operational limits have in like manner been discussed with helpful application by a couple of workers. Beside centrifugation, the mechanical separation in like manner expect an important activity for removing corruptions obtain a comparably sterilized thing, Screening is furthermore a technique used in various structures in the sugar business. The speed keeps up could be around 8000 rpm in the extent of 70-75% mud soddenness. The material vehicle in such a hub can catch suspension particles. The size extent of 1-2µm. It can have a wide extent of feed stream, which can be evaluated dependent on a full-scale. All of these concentrations as discussed for a significant determination of centrifugation of stick juice in the sugar business.

scholarly journals DETERMINATION OF THE REAL VALUES OF KINEMATIC INDEX CORRESPONDING TO THE MECHANICAL SEPARATION PROCESS

2017 ◽  
Vol 22 (2) ◽  
Author(s):  
EMILIAN MOȘNEGUȚU ◽  
VALENTIN NEDEFF ◽  
MIRELA PANAINTE-LEHĂDUȘ ◽  
NARCIS BÂRSAN ◽  
DANA CHIȚIMUȘ ◽  
...  
Keyword(s):  
Separation Process ◽  
Experimental Batch ◽  
Solid Particles ◽  
Common Method ◽  
The Real ◽  
Mechanical Separation

This article presents the methodology for determining the real values of kinematic indices which characterizes the mechanical separation of a mixture of solid particles. Mechanical separation of a mixture of solid-solid on surface provided with holes is the most common method of separation. To optimize equipment that performs this operation, both theoretical and practical characteristics are determined. Kinematic indices are part of the theoretical characteristics, helping to identify behavior of solid particles on a surface flat swing. Starting from an experimental batch, real values of the kinematic indices corresponding to up, down and sideways movement on the sieve were determined for the following types of real particles: grain, large grain beans, small grain beans and soybeans.

Protein Solubilization

2018 ◽  
Vol 95 (8) ◽  
pp. 883-901 ◽  
Author(s):  
Shridhar K. Sathe ◽  
Valerie D. Zaffran ◽  
Sahil Gupta ◽  
Tengfei Li
Keyword(s):  
Protein Solubilization
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