scholarly journals Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

10.3791/61433 ◽  
2020 ◽  
Author(s):  
Joree N. Sandin ◽  
Surya P. Aryal ◽  
Thomas Wilkop ◽  
Christopher I. Richards ◽  
Martha E. Grady
Keyword(s):  
Atomic Force Microscopy ◽  
Laser Scanning ◽  
Live Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals An Atomic Force Microscopy Study of Live Cells and Biofilms of Rhizobium Leguminosarum

2011 ◽  
Vol 100 (3) ◽  
pp. 162a
Author(s):  
Jun Dong ◽  
Elizabeth M. Vanderlinde ◽  
Christopher K. Yost ◽  
Tanya E.S. Dahms
Keyword(s):  
Atomic Force Microscopy ◽  
Rhizobium Leguminosarum ◽  
Microscopy Study ◽  
Live Cells ◽  
Atomic Force Microscopy Study ◽  
Force Microscopy ◽  
Atomic Force

Ultrasonically synthesized organic liquid-filled chitosan microcapsules: part 2: characterization using AFM (atomic force microscopy) and combined AFM–confocal laser scanning fluorescence microscopy

Soft Matter ◽  
2018 ◽  
Vol 14 (16) ◽  
pp. 3192-3201 ◽  
Author(s):  
Srinivas Mettu ◽  
Qianyu Ye ◽  
Meifang Zhou ◽  
Raymond Dagastine ◽  
Muthupandian Ashokkumar
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
Young’S Modulus ◽  
Laser Scanning ◽  
Young's Modulus ◽  
Organic Liquid ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy (AFM) is used to measure the stiffness and Young's modulus of individual microcapsules that have a chitosan cross-linked shell encapsulating tetradecane.

Resonance compensating chirp mode for mapping the rheology of live cells by high-speed atomic force microscopy

2018 ◽  
Vol 113 (9) ◽  
pp. 093701 ◽  
Author(s):  
Marc Schächtele ◽  
Erik Hänel ◽  
Tilman E. Schäffer
Keyword(s):  
Atomic Force Microscopy ◽  
High Speed ◽  
Live Cells ◽  
Force Microscopy ◽  
Atomic Force

Comparison study of live cells by atomic force microscopy, confocal microscopy, and scanning electrochemical microscopy

2007 ◽  
Vol 85 (3) ◽  
pp. 175-183 ◽  
Author(s):  
Xiaocui Zhao ◽  
Nils O Petersen ◽  
Zhifeng Ding
Keyword(s):  
Atomic Force Microscopy ◽  
Confocal Microscopy ◽  
Scanning Electrochemical Microscopy ◽  
Time Lapse ◽  
Live Cells ◽  
Tapping Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Tapping Mode Afm ◽  
Electrochemical Microscopy

In this report, three kinds of scanning probe microscopy techniques, atomic force microscopy (AFM), confocal microscopy (CM), and scanning electrochemical microscopy (SECM), were used to study live cells in the physiological environment. Two model cell lines, CV-1 and COS-7, were studied. Time-lapse images were obtained with both contact and tapping mode AFM techniques. Cells were more easily scratched or moved by contact mode AFM than by tapping mode AFM. Detailed surface structures such as filamentous structures on the cell membrane can be obtained and easily discerned with tapping mode AFM. The toxicity of ferrocenemethanol (Fc) on live cells was studied by CM in reflection mode by recording the time-lapse images of controlled live cells and live cells with different Fc concentrations. No significant change in the morphology of cells was caused by Fc. Cells were imaged by SECM with Fc as the mediator at a biased potential of 0.35 V (vs. Ag/AgCl with a saturated KCl solution). Cells did not change visibly within 1 h, which indicated that SECM was a noninvasive technique and thus has a unique advantage for the study of soft cells, since the electrode scanned above the cells instead of in contact with them. Reactive oxygen species (ROS) generated by the cells were detected and images based on these chemical species were obtained. It is demonstrated that SECM can provide not only the topographical images but also the images related to the chemical or biochemical species released by the live cells.Key words: live cells, atomic force microscopy, confocal microscopy, scanning electrochemical microscopy.

Fusion Pore in Live Cells

Physiology ◽  
2002 ◽  
Vol 17 (6) ◽  
pp. 219-222 ◽  
Author(s):  
Bhanu P. Jena
Keyword(s):  
Atomic Force Microscopy ◽  
Live Cells ◽  
Fusion Pore ◽  
Secretory Cells ◽  
Secretory Vesicles ◽  
Force Microscopy ◽  
Atomic Force ◽  
Electrophysiological Measurements ◽  
First Time ◽  
Fusion Pores

Earlier electrophysiological measurements on live secretory cells suggested the presence of fusion pores at the plasma membrane, where secretory vesicles fuse to release vesicular contents. Recent studies using atomic force microscopy demonstrate for the first time the presence of the fusion pore and reveal its morphology and dynamics at near-nanometer resolution and in real time.

Mapping nanomechanical properties of live cells using multi-harmonic atomic force microscopy

2011 ◽  
Vol 6 (12) ◽  
pp. 809-814 ◽  
Author(s):  
A. Raman ◽  
S. Trigueros ◽  
A. Cartagena ◽  
A. P. Z. Stevenson ◽  
M. Susilo ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Live Cells ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

scholarly journals Actin microridges characterized by laser scanning confocal and atomic force microscopy

FEBS Letters ◽  
2005 ◽  
Vol 579 (9) ◽  
pp. 2001-2008 ◽  
Author(s):  
Amita Sharma ◽  
Kurt I. Anderson ◽  
Daniel J. Müller
Keyword(s):  
Atomic Force Microscopy ◽  
Laser Scanning ◽  
Force Microscopy ◽  
Atomic Force
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