Fusion Pore in Live Cells

Physiology ◽  
2002 ◽  
Vol 17 (6) ◽  
pp. 219-222 ◽  
Author(s):  
Bhanu P. Jena
Keyword(s):  
Atomic Force Microscopy ◽  
Live Cells ◽  
Fusion Pore ◽  
Secretory Cells ◽  
Secretory Vesicles ◽  
Force Microscopy ◽  
Atomic Force ◽  
Electrophysiological Measurements ◽  
First Time ◽  
Fusion Pores

Earlier electrophysiological measurements on live secretory cells suggested the presence of fusion pores at the plasma membrane, where secretory vesicles fuse to release vesicular contents. Recent studies using atomic force microscopy demonstrate for the first time the presence of the fusion pore and reveal its morphology and dynamics at near-nanometer resolution and in real time.

scholarly journals Fusion pore or porosome: structure and dynamics

2003 ◽  
Vol 176 (2) ◽  
pp. 169-174 ◽  
Author(s):  
BP Jena
Keyword(s):  
Plasma Membrane ◽  
Neuroendocrine Cells ◽  
Fusion Pore ◽  
Secretory Cells ◽  
Force Microscopy ◽  
Membrane Pores ◽  
Atomic Force ◽  
Cell Plasma ◽  
Electrophysiological Measurements ◽  
Membrane Bound

Electrophysiological measurements on live secretory cells almost a decade ago suggested the presence of fusion pores at the cell plasma membrane. Membrane-bound secretory vesicles were hypothesized to dock and fuse at these sites, to release their contents. Our studies using atomic force microscopy on live exocrine and neuroendocrine cells demonstrate the presence of such plasma membrane pores, revealing their morphology and dynamics at near nm resolution and in real time.

scholarly journals An Atomic Force Microscopy Study of Live Cells and Biofilms of Rhizobium Leguminosarum

2011 ◽  
Vol 100 (3) ◽  
pp. 162a
Author(s):  
Jun Dong ◽  
Elizabeth M. Vanderlinde ◽  
Christopher K. Yost ◽  
Tanya E.S. Dahms
Keyword(s):  
Atomic Force Microscopy ◽  
Rhizobium Leguminosarum ◽  
Microscopy Study ◽  
Live Cells ◽  
Atomic Force Microscopy Study ◽  
Force Microscopy ◽  
Atomic Force

Resonance compensating chirp mode for mapping the rheology of live cells by high-speed atomic force microscopy

2018 ◽  
Vol 113 (9) ◽  
pp. 093701 ◽  
Author(s):  
Marc Schächtele ◽  
Erik Hänel ◽  
Tilman E. Schäffer
Keyword(s):  
Atomic Force Microscopy ◽  
High Speed ◽  
Live Cells ◽  
Force Microscopy ◽  
Atomic Force

Comparison study of live cells by atomic force microscopy, confocal microscopy, and scanning electrochemical microscopy

2007 ◽  
Vol 85 (3) ◽  
pp. 175-183 ◽  
Author(s):  
Xiaocui Zhao ◽  
Nils O Petersen ◽  
Zhifeng Ding
Keyword(s):  
Atomic Force Microscopy ◽  
Confocal Microscopy ◽  
Scanning Electrochemical Microscopy ◽  
Time Lapse ◽  
Live Cells ◽  
Tapping Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Tapping Mode Afm ◽  
Electrochemical Microscopy

In this report, three kinds of scanning probe microscopy techniques, atomic force microscopy (AFM), confocal microscopy (CM), and scanning electrochemical microscopy (SECM), were used to study live cells in the physiological environment. Two model cell lines, CV-1 and COS-7, were studied. Time-lapse images were obtained with both contact and tapping mode AFM techniques. Cells were more easily scratched or moved by contact mode AFM than by tapping mode AFM. Detailed surface structures such as filamentous structures on the cell membrane can be obtained and easily discerned with tapping mode AFM. The toxicity of ferrocenemethanol (Fc) on live cells was studied by CM in reflection mode by recording the time-lapse images of controlled live cells and live cells with different Fc concentrations. No significant change in the morphology of cells was caused by Fc. Cells were imaged by SECM with Fc as the mediator at a biased potential of 0.35 V (vs. Ag/AgCl with a saturated KCl solution). Cells did not change visibly within 1 h, which indicated that SECM was a noninvasive technique and thus has a unique advantage for the study of soft cells, since the electrode scanned above the cells instead of in contact with them. Reactive oxygen species (ROS) generated by the cells were detected and images based on these chemical species were obtained. It is demonstrated that SECM can provide not only the topographical images but also the images related to the chemical or biochemical species released by the live cells.Key words: live cells, atomic force microscopy, confocal microscopy, scanning electrochemical microscopy.

Mapping nanomechanical properties of live cells using multi-harmonic atomic force microscopy

2011 ◽  
Vol 6 (12) ◽  
pp. 809-814 ◽  
Author(s):  
A. Raman ◽  
S. Trigueros ◽  
A. Cartagena ◽  
A. P. Z. Stevenson ◽  
M. Susilo ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Live Cells ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

scholarly journals A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis

2021 ◽  
Vol 9 (4) ◽  
pp. 680
Author(s):  
Christopher T. Evans ◽  
Sara J. Baldock ◽  
John G. Hardy ◽  
Oliver Payton ◽  
Loren Picco ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
High Speed ◽  
Single Cells ◽  
Sample Surface ◽  
Live Cells ◽  
Contact Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Analysis

Suitable immobilisation of microorganisms and single cells is key for high-resolution topographical imaging and study of mechanical properties with atomic force microscopy (AFM) under physiologically relevant conditions. Sample preparation techniques must be able to withstand the forces exerted by the Z range-limited cantilever tip, and not negatively affect the sample surface for data acquisition. Here, we describe an inherently flexible methodology, utilising the high-resolution three-dimensional based printing technique of multiphoton polymerisation to rapidly generate bespoke arrays for cellular AFM analysis. As an example, we present data collected from live Emiliania huxleyi cells, unicellular microalgae, imaged by contact mode High-Speed Atomic Force Microscopy (HS-AFM), including one cell that was imaged continuously for over 90 min.

scholarly journals Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

10.3791/61433 ◽  
2020 ◽  
Author(s):  
Joree N. Sandin ◽  
Surya P. Aryal ◽  
Thomas Wilkop ◽  
Christopher I. Richards ◽  
Martha E. Grady
Keyword(s):  
Atomic Force Microscopy ◽  
Laser Scanning ◽  
Live Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Reinforcement Mechanism of Carbon Black-Filled Rubber Nanocomposite as Revealed by Atomic Force Microscopy Nanomechanics

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3922
Author(s):  
Xiaobin Liang ◽  
Makiko Ito ◽  
Ken Nakajima
Keyword(s):  
Atomic Force Microscopy ◽  
Stress Distribution ◽  
Carbon Black ◽  
Stress Concentrations ◽  
Force Microscopy ◽  
Reinforcement Mechanism ◽  
Atomic Force ◽  
Filled Rubber ◽  
First Time ◽  
The Relationship

In this study, atomic force microscopy (AFM) nanomechanics were used to visualize the nanoscale stress distribution in carbon black (CB)-reinforced isoprene rubber (IR) vulcanizates at different elongations and quantitatively evaluate their volume fractions for the first time. The stress concentrations in the protofibrous structure (stress chains) that formed around the CB filler in CB-reinforced IR vulcanizates were directly observed at the nanoscale. The relationship between the local nanoscale stress distribution and macroscopic tensile properties was revealed based on the microscopic stress distribution and microscopic spatial structure. This study can help us gain insight into the microscopic reinforcement mechanism of carbon black-containing rubber composites.

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