Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

10.3791/61234 ◽  
2020 ◽  
Author(s):  
Julia Nilsson ◽  
Dan Holmberg ◽  
Anja Schmidt-Christensen
Keyword(s):  
Pancreatic Islet ◽  
In Vivo Imaging ◽  
Host Cells ◽  
Human Pancreatic Islet

scholarly journals In vivo imaging of emerging endocrine cells reveals a requirement for PI3K-regulated motility in pancreatic islet morphogenesis

Development ◽  
2018 ◽  
Vol 145 (3) ◽  
pp. dev158477 ◽  
Author(s):  
Julia Freudenblum ◽  
José A. Iglesias ◽  
Martin Hermann ◽  
Tanja Walsen ◽  
Armin Wilfinger ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
In Vivo Imaging ◽  
Endocrine Cells

scholarly journals Enterovirus infection in human pancreatic islet cells, islet tropism in vivo and receptor involvement in cultured islet beta cells

Diabetologia ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 225-239 ◽  
Author(s):  
P. Ylipaasto ◽  
K. Klingel ◽  
A. M. Lindberg ◽  
T. Otonkoski ◽  
R. Kandolf ◽  
...  
Keyword(s):  
Beta Cells ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Enterovirus Infection ◽  
Islet Beta Cells ◽  
Human Pancreatic Islet

PRESERVATION OF HUMAN PANCREATIC ISLET IN VIVO FUNCTION AFTER 6-MONTH CULTURE IN SERUM-FREE MEDIA1

2004 ◽  
Vol 77 (8) ◽  
pp. 1147-1154 ◽  
Author(s):  
Benjamin T. Rush ◽  
Daniel W. Fraga ◽  
Malak Y. Kotb ◽  
Omaima M. Sabek ◽  
Agnes Lo ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Serum Free ◽  
Human Pancreatic Islet

scholarly journals Use of the PET ligand florbetapir for in vivo imaging of pancreatic islet amyloid deposits in hIAPP transgenic mice

Diabetologia ◽  
2018 ◽  
Vol 61 (10) ◽  
pp. 2215-2224 ◽  
Author(s):  
Andrew T. Templin ◽  
Daniel T. Meier ◽  
Joshua R. Willard ◽  
Tami Wolden-Hanson ◽  
Kelly Conway ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Pancreatic Islet ◽  
In Vivo Imaging ◽  
Islet Amyloid ◽  
Amyloid Deposits

scholarly journals Noninvasive in vivo imaging of pancreatic islet cell biology

2008 ◽  
Vol 14 (5) ◽  
pp. 574-578 ◽  
Author(s):  
Stephan Speier ◽  
Daniel Nyqvist ◽  
Over Cabrera ◽  
Jia Yu ◽  
R Damaris Molano ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
In Vivo Imaging ◽  
Cell Biology ◽  
Islet Cell ◽  
Pancreatic Islet Cell

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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