Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

10.3791/61105 ◽  
2020 ◽  
Author(s):  
Łukasz Makowski ◽  
Damian Trojanowski ◽  
Jolanta Zakrzewska-Czerwińska
Keyword(s):  
Life Cycle ◽  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Time Lapse ◽  
Live Cell ◽  
Bdellovibrio Bacteriovorus

scholarly journals Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Dulanthi Weerasekera ◽  
Jonas Hahn ◽  
Martin Herrmann ◽  
Andreas Burkovski
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Time Lapse ◽  
Corynebacterium Diphtheriae ◽  
Live Cell ◽  
Human Macrophage ◽  
Data Description ◽  
Corynebacterium Ulcerans ◽  
Microscopy Data

Abstract Objectives In frame of a study to characterize the interaction of human macrophage-like cells with pathogenic corynebacteria, Corynebacterium diphtheriae and Corynebacterium ulcerans, live cell imaging experiments were carried out and time lapse fluorescence microscopy videos were generated, which are presented here. Data description The time lapse fluorescence microscopy data revealed new insights in the interaction of corynebacteria with human macrophage-like THP-1 cells. In contrast to uninfected cells and infections with non-pathogenic C. glutamicum used as a control, pathogenic C. diphtheriae and C. ulcerans showed highly detrimental effects towards human cells and induction of cell death of macrophages.

scholarly journals Time-Lapse, Live-Cell Imaging of Potassium Efflux in Cell Exposed to Nanosecond Pulsed Electric Fields

2021 ◽  
Vol 120 (3) ◽  
pp. 223a
Author(s):  
Flavia Mazzarda ◽  
Esin B. Sozer ◽  
Julia L. Pittaluga ◽  
Claudia Muratori ◽  
P. Thomas Vernier
Keyword(s):  
Electric Fields ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Pulsed Electric Fields ◽  
Time Lapse ◽  
Live Cell ◽  
Potassium Efflux ◽  
Nanosecond Pulsed Electric Fields

Dye selection for live cell imaging of intact siRNA

2012 ◽  
Vol 393 (1-2) ◽  
pp. 23-35 ◽  
Author(s):  
Markus Hirsch ◽  
Dennis Strand ◽  
Mark Helm
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Time Lapse ◽  
Live Cell ◽  
High Yield ◽  
Ph Variation ◽  
Rnai Efficiency

Abstract Investigations into the fate of small interfering RNA (siRNA) after transfection may unravel new ways to improve RNA interference (RNAi) efficiency. Because intracellular degradation of RNA may prevent reliable observation of fluorescence-labeled siRNA, new tools for fluorescence microscopy are warranted to cover the considerable duration of the RNAi effect. Here, the characterization and application of new fluorescence resonance energy transfer (FRET) dye pairs for sensing the integrity of duplex siRNA is reported, which allows an assessment of the degradation status of an siRNA cell population by live cell imaging. A panel of high-yield fluorescent dyes has been investigated for their suitability as FRET pairs for the investigation of RNA inside the cell. Nine dyes in 13 FRET pairs were evaluated based on the performance in assays of photostability, cross-excitation, bleed-through, as well as on quantified changes of fluorescence as a consequence of, e.g., RNA strand hybridization and pH variation. The Atto488/Atto590 FRET pair has been applied to live cell imaging, and has revealed first aspects of unusual trafficking of intact siRNA. A time-lapse study showed highly dynamic movement of siRNA in large perinuclear structures. These and the resulting optimized FRET labeled siRNA are expected to have significant impact on future observations of labeled RNAs in living cells.

scholarly journals Full-field supercritical angle fluorescence microscopy for live cell imaging

2011 ◽  
Vol 36 (16) ◽  
pp. 3051 ◽  
Author(s):  
Thomas Barroca ◽  
Karla Balaa ◽  
Julie Delahaye ◽  
Sandrine Lévêque-Fort ◽  
Emmanuel Fort
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Full Field

scholarly journals Time-lapse live cell imaging to monitor doxorubicin release from DNA origami nanostructures

2018 ◽  
Vol 6 (11) ◽  
pp. 1605-1612 ◽  
Author(s):  
Yun Zeng ◽  
Jiajun Liu ◽  
Shuo Yang ◽  
Wenyan Liu ◽  
Liang Xu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Time Lapse ◽  
Dna Origami ◽  
Live Cell ◽  
Doxorubicin Release

DNA origami nanostructures can serve as a promising carrier for drug delivery due to the outstanding programmability and biocompatibility.

scholarly journals Ionomycin-induced calcium influx induces neurite degeneration in mouse neuroblastoma cells: analysis of a time-lapse live cell imaging system

2016 ◽  
Vol 50 (11) ◽  
pp. 1214-1225 ◽  
Author(s):  
Saki Nakamura ◽  
Ayumi Nakanishi ◽  
Minami Takazawa ◽  
Shunsuke Okihiro ◽  
Shiro Urano ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Imaging System ◽  
Calcium Influx ◽  
Neuroblastoma Cells ◽  
Time Lapse ◽  
Live Cell ◽  
Mouse Neuroblastoma ◽  
Neurite Degeneration

scholarly journals Live cell imaging of the HIV-1 life cycle

2008 ◽  
Vol 16 (12) ◽  
pp. 580-587 ◽  
Author(s):  
Edward M. Campbell ◽  
Thomas J. Hope
Keyword(s):  
Life Cycle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document