scholarly journals Live cell imaging of the HIV-1 life cycle

2008 ◽  
Vol 16 (12) ◽  
pp. 580-587 ◽  
Author(s):  
Edward M. Campbell ◽  
Thomas J. Hope
Keyword(s):  
Life Cycle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Hiv 1

Cost-Effective High-Speed, Three-Dimensional Live-Cell Imaging of HIV-1 Transfer at the T Cell Virological Synapse

2021 ◽  
Author(s):  
Alice Sandmeyer ◽  
Lili Wang ◽  
Wolfgang Hübner ◽  
Marcel Müller ◽  
Benjamin Chen ◽  
...  
Keyword(s):  
T Cell ◽  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Three Dimensional ◽  
Cost Effective ◽  
Live Cell ◽  
Virological Synapse ◽  
Hiv 1

scholarly journals Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly

Viruses ◽  
2012 ◽  
Vol 4 (5) ◽  
pp. 777-799 ◽  
Author(s):  
Viola Baumgärtel ◽  
Barbara Müller ◽  
Don C. Lamb
Keyword(s):  
Human Immunodeficiency Virus ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Immunodeficiency Virus ◽  
Hiv 1

Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot–peptide complex

2010 ◽  
Vol 46 (48) ◽  
pp. 9146 ◽  
Author(s):  
Youngseon Choi ◽  
Junghan Lee ◽  
Keumhyun Kim ◽  
Heeyeon Kim ◽  
Peter Sommer ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Protease Activity ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Peptide Complex ◽  
Stable Quantum ◽  
Acid Stable ◽  
Hiv 1

Live Cell Imaging of the Schizosaccharomyces pombe Sexual Life Cycle

2017 ◽  
Vol 2017 (10) ◽  
pp. pdb.prot090225 ◽  
Author(s):  
Laura Merlini ◽  
Aleksandar Vjestica ◽  
Omaya Dudin ◽  
Felipe Bendezú ◽  
Sophie G. Martin
Keyword(s):  
Life Cycle ◽  
Schizosaccharomyces Pombe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Sexual Life ◽  
Sexual Life Cycle

scholarly journals A Versatile Tool for Live-Cell Imaging and Super-Resolution Nanoscopy Studies of HIV-1 Env Distribution and Mobility

2017 ◽  
Vol 24 (5) ◽  
pp. 635-645.e5 ◽  
Author(s):  
Volkan Sakin ◽  
Janina Hanne ◽  
Jessica Dunder ◽  
Maria Anders-Össwein ◽  
Vibor Laketa ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Versatile Tool ◽  
Hiv 1

scholarly journals Kinesin-8B controls basal body function and flagellum formation and is key to malaria parasite transmission

2019 ◽  
Author(s):  
Mohammad Zeeshan ◽  
David J. P. Ferguson ◽  
Steven Abel ◽  
Alana Burrrell ◽  
Edward Rea ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Life Cycle ◽  
Live Cell Imaging ◽  
Malaria Parasite ◽  
Cell Imaging ◽  
Male Gamete ◽  
Live Cell ◽  
Basal Bodies ◽  
Parasite Transmission ◽  
Parasite Life Cycle

AbstractEukaryotic flagella are conserved microtubule-based organelles that drive cell motility. Plasmodium, the causative agent of malaria, has a single flagellate stage: the male gamete in the mosquito. Three rounds of endomitotic division together with an unusual mode of flagellum assembly rapidly produce eight motile gametes. These processes are tightly coordinated but their regulation is poorly understood. To understand this important developmental stage, we studied the function and location of the microtubule-based motor kinesin-8B, using gene-targeting, electron microscopy and live cell imaging. Deletion of the kinesin-8B gene showed no effect on mitosis but disrupted 9+2 axoneme assembly and flagellum formation during male gamete development and also completely ablated parasite transmission. Live cell imaging showed that kinesin-8B-GFP did not colocalise with kinetochores in the nucleus but instead revealed dynamic, cytoplasmic localisation with the basal bodies and the assembling axoneme during flagellum formation. We thus uncovered an unexpected role for kinesin-8B in parasite flagellum formation that is vital for the parasite life cycle.

scholarly journals Spatial and Temporal Analysis of Alphavirus Replication and Assembly in Mammalian and Mosquito Cells

mBio ◽  
2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Joyce Jose ◽  
Aaron B. Taylor ◽  
Richard J. Kuhn
Keyword(s):  
Electron Microscopy ◽  
Life Cycle ◽  
Virus Replication ◽  
Mammalian Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Virus Production ◽  
Live Cell ◽  
Persistently Infected ◽  
Mosquito Cells

ABSTRACT Sindbis virus (SINV [genus Alphavirus , family Togaviridae ]) is an enveloped, mosquito-borne virus. Alphaviruses cause cytolytic infections in mammalian cells while establishing noncytopathic, persistent infections in mosquito cells. Mosquito vector adaptation of alphaviruses is a major factor in the transmission of epidemic strains of alphaviruses. Though extensive studies have been performed on infected mammalian cells, the morphological and structural elements of alphavirus replication and assembly remain poorly understood in mosquito cells. Here we used high-resolution live-cell imaging coupled with single-particle tracking and electron microscopy analyses to delineate steps in the alphavirus life cycle in both the mammalian host cell and insect vector cells. Use of dually labeled SINV in conjunction with cellular stains enabled us to simultaneously determine the spatial and temporal differences of alphavirus replication complexes (RCs) in mammalian and insect cells. We found that the nonstructural viral proteins and viral RNA in RCs exhibit distinct spatial organization in mosquito cytopathic vacuoles compared to replication organelles from mammalian cells. We show that SINV exploits filopodial extensions for virus dissemination in both cell types. Additionally, we propose a novel mechanism for replication complex formation around glycoprotein-containing vesicles in mosquito cells that produced internally released particles that were seen budding from the vesicles by live imaging. Finally, by characterizing mosquito cell lines that were persistently infected with fluorescent virus, we show that the replication and assembly machinery are highly modified, and this allows continuous production of alphaviruses at reduced levels. IMPORTANCE Reemerging mosquito-borne alphaviruses cause serious human epidemics worldwide. Several structural and imaging studies have helped to define the life cycle of alphaviruses in mammalian cells, but the mode of virus replication and assembly in the invertebrate vector and mechanisms producing two disease outcomes in two types of cells are yet to be identified. Using transmission electron microscopy and live-cell imaging with dual fluorescent protein-tagged SINV, we show that while insect and mammalian cells display similarities in entry and exit, they present distinct spatial and temporal organizations in virus replication and assembly. By characterizing acutely and persistently infected cells, we provide new insights into alphavirus replication and assembly in two distinct hosts, resulting in high-titer virus production in mammalian cells and continuous virus production at reduced levels in mosquito cells—presumably a prerequisite for alphavirus maintenance in nature.

scholarly journals Live-Cell Imaging of Early Steps of Single HIV-1 Infection

Viruses ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 275 ◽  
Author(s):  
Ashwanth Francis ◽  
Gregory Melikyan
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Hiv 1

scholarly journals Live Cell Imaging of ABCA1 Downregulation by HIV-1 Nef in an Experimental Model of HeLa ABCA1-GFP

2016 ◽  
Vol 32 (9) ◽  
pp. 872-873
Author(s):  
Johanna Siegel ◽  
Christina Darwish ◽  
Anastas Popratiloff ◽  
Michael Bukrinsky ◽  
Beda Brichacek
Keyword(s):  
Experimental Model ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document