A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

10.3791/60955 ◽  
2020 ◽  
Author(s):  
Amir Bagheri ◽  
Seyedeh Fatemeh Razavipour ◽  
Claes Wahlestedt ◽  
Seyed Javad Mowla ◽  
Mohammad Ali Faghihi
Keyword(s):  
Neurite Outgrowth ◽  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Human Neural Progenitor Cell

scholarly journals Front Cover: In Vitro and In Vivo Proteomic Comparison of Human Neural Progenitor Cell‐Induced Photoreceptor Survival

PROTEOMICS ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 1970011
Author(s):  
Melissa K. Jones ◽  
Bin Lu ◽  
Dawn Zhaohui Chen ◽  
Weston R. Spivia ◽  
Augustus T. Mercado ◽  
...  
Keyword(s):  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Front Cover ◽  
Human Neural Progenitor Cell

scholarly journals Differentiation of human neural progenitor cell-derived spiral ganglion-like neurons: a time-lapse video study

2014 ◽  
Vol 134 (5) ◽  
pp. 441-447 ◽  
Author(s):  
Fredrik Edin ◽  
Wei Liu ◽  
Marja Boström ◽  
Peetra U. Magnusson ◽  
Helge Rask-Andersen
Keyword(s):  
Progenitor Cell ◽  
Spiral Ganglion ◽  
Time Lapse ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Video Study ◽  
Human Neural Progenitor Cell ◽  
Time Lapse Video

scholarly journals Quantitative and kinetic profile of Wnt/β-catenin signaling components during human neural progenitor cell differentiation

2011 ◽  
Vol 16 (4) ◽  
Author(s):  
Orianne Mazemondet ◽  
Rayk Hubner ◽  
Jana Frahm ◽  
Dirk Koczan ◽  
Benjamin Bader ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Progenitor Cell ◽  
Target Genes ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Signaling Molecules ◽  
Human Neural Progenitor Cell ◽  
Wnt Ligands ◽  
Spatio Temporal

AbstractReNcell VM is an immortalized human neural progenitor cell line with the ability to differentiate in vitro into astrocytes and neurons, in which the Wnt/β-catenin pathway is known to be involved. However, little is known about kinetic changes of this pathway in human neural progenitor cell differentiation. In the present study, we provide a quantitative profile of Wnt/β-catenin pathway dynamics showing its spatio-temporal regulation during ReNcell VM cell differentiation. We show first that T-cell factor dependent transcription can be activated by stabilized β-catenin. Furthermore, endogenous Wnt ligands, pathway receptors and signaling molecules are temporally controlled, demonstrating changes related to differentiation stages. During the first three hours of differentiation the signaling molecules LRP6, Dvl2 and β-catenin are spatio-temporally regulated between distinct cellular compartments. From 24 h onward, components of the Wnt/β-catenin pathway are strongly activated and regulated as shown by mRNA up-regulation of Wnt ligands (Wnt5a and Wnt7a), receptors including Frizzled-2, -3, -6, -7, and -9, and co-receptors, and target genes including Axin2. This detailed temporal profile of the Wnt/β-catenin pathway is a first step to understand, control and to orientate, in vitro, human neural progenitor cell differentiation.

In vitro characterization of a human neural progenitor cell coexpressing SSEA4 and CD133

2007 ◽  
Vol 85 (2) ◽  
pp. 250-259 ◽  
Author(s):  
Perrine Barraud ◽  
Simon Stott ◽  
Kjeld Møllgård ◽  
Malin Parmar ◽  
Anders Björklund
Keyword(s):  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
In Vitro Characterization ◽  
Human Neural Progenitor Cell
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