Development of a Larval Zebrafish Infection Model for Clostridioides difficile

10.3791/60793 ◽  
2020 ◽  
Author(s):  
Junkai Li ◽  
Can M. Ünal ◽  
Kazuhiko Namikawa ◽  
Michael Steinert ◽  
Reinhard W. Köster
Keyword(s):  
Infection Model ◽  
Larval Zebrafish ◽  
Clostridioides Difficile ◽  
Zebrafish Infection

scholarly journals Mycobacterium marinum infection drives foam cell differentiation in zebrafish infection

2018 ◽  
Author(s):  
Matt D. Johansen ◽  
Joshua A. Kasparian ◽  
Elinor Hortle ◽  
Warwick J. Britton ◽  
Auriol C. Purdie ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Lipid Metabolism ◽  
Foam Cell ◽  
Mycobacterial Infection ◽  
Foam Cells ◽  
Mycobacterium Marinum ◽  
Infection Model ◽  
Structural Components ◽  
Important Target ◽  
Zebrafish Infection

AbstractHost lipid metabolism is an important target for subversion by pathogenic mycobacteria such as Mycobacterium tuberculosis. The appearance of foam cells within the granuloma are well-characterised effects of chronic tuberculosis. The zebrafish-Mycobacterium marinum infection model recapitulates many aspects of human-M. tuberculosis infection and is used as a model to investigate the structural components of the mycobacterial granuloma. Here, we demonstrate that the zebrafish-M. marinum granuloma contains foam cells and that the transdifferentiation of macrophages into foam cells is driven by the mycobacterial ESX1 pathogenicity locus. This report demonstrates conservation of an important aspect of mycobacterial infection across species.

scholarly journals Occurrence of foamy macrophages during the innate response of zebrafish to trypanosome infections

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Sem H Jacobs ◽  
Eva Dóró ◽  
Ffion R Hammond ◽  
Mai E Nguyen-Chi ◽  
Georges Lutfalla ◽  
...  
Keyword(s):  
Immune Response ◽  
Early Response ◽  
Innate Immune ◽  
Infection Model ◽  
Parasite Control ◽  
Innate Response ◽  
Larval Zebrafish ◽  
Inflammatory Profile ◽  
Foamy Macrophages

A tightly regulated innate immune response to trypanosome infections is critical to strike a balance between parasite control and inflammation-associated pathology. In this study, we make use of the recently established Trypanosoma carassii infection model in larval zebrafish to study the early response of macrophages and neutrophils to trypanosome infections in vivo. We consistently identified high- and low-infected individuals and were able to simultaneously characterize their differential innate response. Not only did macrophage and neutrophil number and distribution differ between the two groups, but also macrophage morphology and activation state. Exclusive to high-infected zebrafish, was the occurrence of foamy macrophages characterized by a strong pro-inflammatory profile and potentially associated with an exacerbated immune response as well as susceptibility to the infection. To our knowledge this is the first report of the occurrence of foamy macrophages during an extracellular trypanosome infection.

scholarly journals Bioactive Molecule from Streptomyces sp. Mitigates MDR Klebsiella pneumoniae in Zebrafish Infection Model

2017 ◽  
Vol 8 ◽  
Author(s):  
Lalitha Cheepurupalli ◽  
Thiagarajan Raman ◽  
Sudarshan S. Rathore ◽  
Jayapradha Ramakrishnan
Keyword(s):  
Klebsiella Pneumoniae ◽  
Infection Model ◽  
Streptomyces Sp ◽  
Bioactive Molecule ◽  
Zebrafish Infection

Salmonella plasmid virulence gene spvB enhances bacterial virulence by inhibiting autophagy in a zebrafish infection model

2016 ◽  
Vol 49 ◽  
pp. 252-259 ◽  
Author(s):  
Yuan-yuan Li ◽  
Ting Wang ◽  
Song Gao ◽  
Guang-mei Xu ◽  
Hua Niu ◽  
...  
Keyword(s):  
Virulence Gene ◽  
Bacterial Virulence ◽  
Infection Model ◽  
Zebrafish Infection

Guide to the Larval Zebrafish‐ Aspergillus Infection Model

2021 ◽  
Vol 1 (12) ◽  
Author(s):  
Taylor J. Schoen ◽  
Anna Huttenlocher ◽  
Nancy P. Keller
Keyword(s):  
Infection Model ◽  
Aspergillus Infection ◽  
Larval Zebrafish

Dithiazole thione derivative as competitive NorA efflux pump inhibitor to curtail multi drug resistant clinical isolate of MRSA in a zebrafish infection model

2016 ◽  
Vol 100 (21) ◽  
pp. 9265-9281 ◽  
Author(s):  
Rene Christena Lowrence ◽  
Thiagarajan Raman ◽  
Himesh V. Makala ◽  
Venkatasubramanian Ulaganathan ◽  
Selva Ganesan Subramaniapillai ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Efflux Pump ◽  
Infection Model ◽  
Drug Resistant ◽  
Efflux Pump Inhibitor ◽  
Zebrafish Infection
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