scholarly journals Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

10.3791/60400 ◽  
2020 ◽  
Author(s):  
Mary S. Pampusch ◽  
Pamela J. Skinner
Keyword(s):  
T Cells ◽  
Central Memory ◽  
Memory Phenotype ◽  
Central Memory Phenotype

scholarly journals Central memory phenotype drives success of checkpoint inhibition in combination with CAR T cells

2020 ◽  
Vol 59 (7) ◽  
pp. 724-735 ◽  
Author(s):  
Karin Toews ◽  
Laura Grunewald ◽  
Silke Schwiebert ◽  
Anika Klaus ◽  
Annika Winkler ◽  
...  
Keyword(s):  
T Cells ◽  
Central Memory ◽  
Checkpoint Inhibition ◽  
Memory Phenotype ◽  
Car T Cells ◽  
Car T ◽  
Central Memory Phenotype

scholarly journals Manufacture of Gene-Modified Human T-Cells with a Memory Stem/Central Memory Phenotype

2014 ◽  
Vol 25 (5) ◽  
pp. 277-287 ◽  
Author(s):  
Raquel Gomez-Eerland ◽  
Bastiaan Nuijen ◽  
Bianca Heemskerk ◽  
Nienke van Rooij ◽  
Joost H. van den Berg ◽  
...  
Keyword(s):  
T Cells ◽  
Central Memory ◽  
Memory Phenotype ◽  
Human T Cells ◽  
Central Memory Phenotype

scholarly journals Rapid GMP-compliant expansion of SARS-CoV-2-specific T cells from convalescent donors for use as an allogeneic cell therapy for COVID-19

2020 ◽  
Author(s):  
Rachel S Cooper ◽  
Alasdair R Fraser ◽  
Linda Smith ◽  
Paul Burgoyne ◽  
Stuart N Imlach ◽  
...  
Keyword(s):  
T Cells ◽  
Cellular Therapy ◽  
Central Memory ◽  
Adoptive Cellular Therapy ◽  
Memory Phenotype ◽  
Allogeneic Cell Therapy ◽  
Membrane Antigens ◽  
Therapeutic Doses ◽  
Manufacturing Yield ◽  
Central Memory Phenotype

AbstractCOVID-19 disease caused by the SARS-CoV-2 virus is characterized by dysregulation of effector T cells and accumulation of exhausted T cells. T cell responses to viruses can be corrected by adoptive cellular therapy using donor-derived virus-specific T cells. Here we show that SARS-CoV-2-exposed blood donations contain CD4 and CD8 memory T cells specific for SARS-CoV-2 spike, nucleocapsid and membrane antigens. These peptides can be used to isolate virus-specific T cells in a GMP-compliant process. These T cells can be rapidly expanded using GMP-compliant reagents for use as a therapeutic product. Memory and effector phenotypes are present in the selected virus-specific T cells, but our method rapidly expands the desirable central memory phenotype. A manufacturing yield ranging from 1010 to 1011 T cells can be obtained within 21 days culture. Thus, multiple therapeutic doses of virus-specific T cells can be rapidly generated from convalescent donors for treatment of COVID-19 patientsOne Sentence SummaryCD4+ and CD8+ T cells specific for SARS-CoV-2 can be isolated from convalescent donors and rapidly expanded to therapeutic doses at GMP standard, maintaining the desired central memory phenotype required for protective immune responses against severe COVID-19 infections.

scholarly journals Peripheral S‐Phase T Cells in HIV Disease Have a Central Memory Phenotype and Rarely Have Evidence of Recent T Cell Receptor Engagement

2005 ◽  
Vol 192 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Scott F. Sieg ◽  
Benigno Rodriguez ◽  
Robert Asaad ◽  
Wei Jiang ◽  
Douglas A. Bazdar ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
S Phase ◽  
Central Memory ◽  
Hiv Disease ◽  
Memory Phenotype ◽  
Central Memory Phenotype

scholarly journals AKT inhibition mitigates terminal differentiation and preserves central memory phenotype of CD8 T cells

2014 ◽  
Vol 2 (Suppl 3) ◽  
pp. P93
Author(s):  
Rasha Abu Eid ◽  
Kevin Friedman ◽  
Mikayel Mkrtichyan ◽  
Samir N Khleif
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Terminal Differentiation ◽  
Central Memory ◽  
Memory Phenotype ◽  
Central Memory Phenotype
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