Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

10.3791/60329 ◽  
2019 ◽  
Author(s):  
Michael Vilkhovoy ◽  
David Dai ◽  
Sandra Vadhin ◽  
Abhinav Adhikari ◽  
Jeffrey D. Varner
Keyword(s):  
Mass Spectrometry ◽  
Protein Synthesis ◽  
Liquid Chromatography ◽  
Absolute Quantification ◽  
Reversed Phase ◽  
Liquid Chromatography Mass Spectrometry ◽  
Reversed Phase Liquid Chromatography ◽  
Free Protein ◽  
Phase Liquid ◽  
Cell Free Protein Synthesis

On-line reversed phase liquid chromatography-mass spectrometry

1979 ◽  
Vol 51 (14) ◽  
pp. 2324-2328 ◽  
Author(s):  
B. L. Karger ◽  
D. P. Kirby ◽  
Paul. Vouros ◽  
R. L. Foltz ◽  
B. Hidy
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Reversed Phase ◽  
Liquid Chromatography Mass Spectrometry ◽  
Reversed Phase Liquid Chromatography ◽  
Chromatography Mass Spectrometry ◽  
On Line ◽  
Phase Liquid

scholarly journals Structural elucidation of triacylglycerol using online acetone Paternò–Büchi reaction coupled with reversed-phase liquid chromatography mass spectrometry

The Analyst ◽  
2020 ◽  
Vol 145 (20) ◽  
pp. 6532-6540 ◽  
Author(s):  
Elissia T. Franklin ◽  
Yu Xia
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Large Scale ◽  
Reversed Phase ◽  
Structural Elucidation ◽  
Scale Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Reversed Phase Liquid Chromatography ◽  
Large Scale Analysis ◽  
Phase Liquid

The developed online RPLC-PB-MS/MS system allows large scale analysis of isomeric triacylglycerol lipids differing in CC locations.

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