scholarly journals Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

10.3791/59455 ◽  
2019 ◽  
Author(s):  
Snorre Gulla ◽  
Saima Nasrin Mohammad ◽  
Duncan John Colquhoun
Keyword(s):  
Capillary Electrophoresis ◽  
Multiplex Pcr ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Pathogenic Bacterium ◽  
Yersinia Ruckeri ◽  
Repeat Analysis

Multiple-locus variable-number tandem repeat analysis of Legionella pneumophila using multi-colored capillary electrophoresis

2008 ◽  
Vol 73 (2) ◽  
pp. 111-117 ◽  
Author(s):  
Alexander J. Nederbragt ◽  
Anusha Balasingham ◽  
Reidun Sirevåg ◽  
Hans Utkilen ◽  
Kjetill S. Jakobsen ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Tandem Repeat ◽  
Legionella Pneumophila ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

scholarly journals Multilocus Variable-Number Tandem-Repeat Analysis ofYersinia ruckeriConfirms the Existence of Host Specificity, Geographic Endemism, and Anthropogenic Dissemination of Virulent Clones

2018 ◽  
Vol 84 (16) ◽  
Author(s):  
Snorre Gulla ◽  
Andrew C. Barnes ◽  
Timothy J. Welch ◽  
Jesús L. Romalde ◽  
David Ryder ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Tandem Repeat ◽  
Minimum Spanning Tree ◽  
Clonal Complex ◽  
Variable Number Tandem Repeat ◽  
Geographic Origin ◽  
Variable Number ◽  
Yersinia Ruckeri ◽  
Content Type ◽  
Repeat Analysis

ABSTRACTA multilocus variable-number tandem-repeat analysis (MLVA) assay was developed for epizootiological study of the internationally significant fish pathogenYersinia ruckeri, which causes yersiniosis in salmonids. The assay involves amplification of 10 variable-number tandem-repeat (VNTR) loci in two five-plex PCRs, followed by capillary electrophoresis. A collection of 484Y. ruckeriisolates, originating from various biological sources and collected from four continents over 7 decades, was analyzed. Minimum-spanning-tree cluster analysis of MLVA profiles separated the studied population into nine major clonal complexes and a number of minor clusters and singletons. The major clonal complexes could be associated with host species, geographic origin, and serotype. A single large clonal complex of serotype O1 isolates dominating the yersiniosis situation in international rainbow trout farming suggests anthropogenic spread of this clone, possibly related to transport of fish. Moreover, subclustering within this clonal complex indicates putative transmission routes and multiple biotype shift events. In contrast to the situation in rainbow trout,Y. ruckeristrains associated with disease in Atlantic salmon appear as more or less geographically isolated clonal complexes. A single complex of serotype O1 exclusive to Norway was found to be responsible for almost all major yersiniosis outbreaks in modern Norwegian salmon farming, and site-specific subclustering further indicates persistent colonization of freshwater farms in Norway. Identification of genetically diverseY. ruckeriisolates from clinically healthy fish and environmental sources also suggests the widespread existence of less-virulent or avirulent strains.IMPORTANCEThis comprehensive population study substantially improves our understanding of the epizootiological history and nature of an internationally important fish-pathogenic bacterium. The MLVA assay developed and presented represents a high-resolution typing tool particularly well suited forYersinia ruckeriinfection tracing, selection of strains for vaccine inclusion, and risk assessment. The ability of the assay to separate isolates into geographically linked and/or possibly host-specific clusters reflects its potential utility for maintenance of national biosecurity. The MLVA is internationally applicable and robust, and it provides clear, unambiguous, and easily interpreted results. Typing is reasonably inexpensive, with a moderate technological requirement, and may be completed from a harvested colony within a single working day. As the resulting MLVA profiles are readily portable, anyY. ruckeristrain may rapidly be placed in a global epizootiological context.

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