Multilaboratory Validation Study of Standardized Multiple-Locus Variable-Number Tandem Repeat Analysis Protocol for Shiga Toxin–ProducingEscherichia coliO157: A Novel Approach to Normalize Fragment Size Data Between Capillary Electrophoresis Platforms

2010 ◽  
Vol 7 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Eija Hyytia-Trees ◽  
Patricia Lafon ◽  
Paul Vauterin ◽  
Efrain M. Ribot
Keyword(s):  
Capillary Electrophoresis ◽  
Tandem Repeat ◽  
Shiga Toxin ◽  
Fragment Size ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Repeat Analysis ◽  
Novel Approach ◽  
Size Data ◽  
Analysis Protocol

scholarly journals Multiple-locus variable-number tandem repeat analysis for strain discrimination of non-O157 Shiga toxin-producing Escherichia coli

2016 ◽  
Vol 125 ◽  
pp. 70-80 ◽  
Author(s):  
Chris Timmons ◽  
Eija Trees ◽  
Efrain M. Ribot ◽  
Peter Gerner-Smidt ◽  
Patti LaFon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tandem Repeat ◽  
Shiga Toxin ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

Multiple-locus variable-number tandem repeat analysis of Legionella pneumophila using multi-colored capillary electrophoresis

2008 ◽  
Vol 73 (2) ◽  
pp. 111-117 ◽  
Author(s):  
Alexander J. Nederbragt ◽  
Anusha Balasingham ◽  
Reidun Sirevåg ◽  
Hans Utkilen ◽  
Kjetill S. Jakobsen ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Tandem Repeat ◽  
Legionella Pneumophila ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

scholarly journals Outbreak ofEscherichia coliO157 Phage Type 32 linked to the consumption of venison products

2018 ◽  
Vol 146 (15) ◽  
pp. 1922-1927 ◽  
Author(s):  
A. Smith-Palmer ◽  
G. Hawkins ◽  
L. Browning ◽  
L. Allison ◽  
M. Hanson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tandem Repeat ◽  
Shiga Toxin ◽  
Phage Type ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Food History ◽  
Repeat Analysis ◽  
Asymptomatic Case ◽  
The Uk

AbstractIn September 2015, an outbreak ofEscherichia coliPhage Type 32 with an indistinguishable multi locus variable number tandem repeat analysis profile was identified in Scotland. Twelve cases were identified; nine primary cases, two secondary and one asymptomatic case. Extensive food history investigations identified venison products containing wild venison produced by a single food business operator as the most likely source of the outbreak. Of the nine primary cases, eight had consumed venison products, and one case had not eaten venison themselves but had handled and cooked raw venison in the household. This was the first reported outbreak of Shiga toxin-producingEscherichia coli(STEC) linked to venison products in the UK, and was also notable due to the implicated products being commercially produced and widely distributed. In contrast, previous venison outbreaks reported from other countries have tended to be smaller and related to individually prepared carcases. The outbreak has highlighted some important knowledge gaps in relation to STEC in venison that are currently been investigated via a number of research studies.

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