A Labor-saving and Repeatable Touch-force Signaling Mutant Screen Protocol for the Study of Thigmomorphogenesis of a Model Plant Arabidopsis thaliana

10.3791/59392 ◽  
2019 ◽  
Author(s):  
Kai Wang ◽  
Kayin Law ◽  
Manhin Leung ◽  
Waishing Wong ◽  
Ning Li
Keyword(s):  
Arabidopsis Thaliana ◽  
Mutant Screen ◽  
Model Plant

scholarly journals Fused Graphical Lasso Recovers Flowering Time Mutation Genes in Arabidopsis Thaliana

Inventions ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 52
Author(s):  
Rajan Kapoor ◽  
Aniruddha Datta ◽  
Michael Thomson
Keyword(s):  
Arabidopsis Thaliana ◽  
Crop Improvement ◽  
Phenotypic Traits ◽  
The Novel ◽  
Model Plant ◽  
Graphical Lasso ◽  
Analysis Strategy ◽  
Model Plant Arabidopsis Thaliana ◽  
Beneficial Traits ◽  
Delayed Flowering

Conventional breeding approaches that focus on yield under highly favorable nutrient conditions have resulted in reduced genetic and trait diversity in crops. Under the growing threat from climate change, the mining of novel genes in more resilient varieties can help dramatically improve trait improvement efforts. In this work, we propose the use of the joint graphical lasso for discovering genes responsible for desired phenotypic traits. We prove its efficiency by using gene expression data for wild type and delayed flowering mutants for the model plant. Arabidopsis thaliana shows that it recovers the mutation causing genes LNK1 and LNK2. Some novel interactions of these genes were also predicted. Observing the network level changes between two phenotypes can also help develop meaningful biological hypotheses regarding the novel functions of these genes. Now that this data analysis strategy has been validated in a model plant, it can be extended to crop plants to help identify the key genes for beneficial traits for crop improvement.

scholarly journals The scope of flavin-dependent reactions and processes in the model plant Arabidopsis thaliana

2021 ◽  
Vol 189 ◽  
pp. 112822
Author(s):  
Reinmar Eggers ◽  
Alexandra Jammer ◽  
Shalinee Jha ◽  
Bianca Kerschbaumer ◽  
Majd Lahham ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Model Plant ◽  
Model Plant Arabidopsis Thaliana ◽  
Plant Arabidopsis Thaliana

scholarly journals A short history of Arabidopsis thaliana (L.) Heynh. Columbia-0

2018 ◽  
Author(s):  
Marc Somssich
Keyword(s):  
Arabidopsis Thaliana ◽  
Short History ◽  
Model Plant ◽  
Natural Accession ◽  
History Of

75 Years ago Arabidopsis thaliana was first suggested as a Model Plant – But how did Arabidopsis Col-0 become the standard natural accession? This is a short history of Arabidopsis thaliana (L.) Heynh. Columbia-0.

Non-host resistance responses of Arabidopsis thaliana to the coffee leaf rust fungus (Hemileia vastatrix)

Botany ◽  
2010 ◽  
Vol 88 (7) ◽  
pp. 621-629 ◽  
Author(s):  
Helena Gil Azinheira ◽  
Maria do Céu Silva ◽  
Pedro Talhinhas ◽  
Clara Medeira ◽  
Isabel Maia ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Rust ◽  
Rust Fungus ◽  
Hemileia Vastatrix ◽  
Callose Deposition ◽  
Mesophyll Cells ◽  
Model Plant ◽  
Heterologous System ◽  
Coffee Leaf Rust ◽  
Model Plant Arabidopsis Thaliana

Leaf rust, caused by Hemileia vastatrix Berk & Broome, is the most destructive fungal disease of coffee. In the absence of a suitable gene validation system in coffee, the objective of this study was to investigate whether the model plant Arabidopsis thaliana (L.) Heynh. may be used as a heterologous system for the molecular dissection of coffee responses to leaf rust. Histological examination of A. thaliana (Col-0) leaves inoculated with H. vastatrix (race II) showed that by 24 h after inoculation (hai), H. vastatrix uredospores differentiated appressoria and penetrated the stomata, but failed to form haustoria. Arabidopsis thaliana cellular resistance responses included hypersensitive-like response (HR) of stomata guard cells together with accumulation of phenolic compounds and callose deposition in walls of epidermal and mesophyll cells. Results indicate that H. vastatrix infection triggered the induction of a set of defence-related genes peaking at 18 and 42 hai. The non-host HR triggered by H. vastatrix in the model plant A. thaliana makes it usable to infer the function of coffee genes involved in pre-haustorial rust resistance.

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