scholarly journals Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

10.3791/57688 ◽  
2018 ◽  
Author(s):  
Michael S. Yu ◽  
Sean Spiering ◽  
Alexandre R. Colas
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Cardiac Progenitors ◽  
Heart Field ◽  
First Heart Field

A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells

2013 ◽  
Vol 15 (9) ◽  
pp. 1098-1106 ◽  
Author(s):  
Daniela Später ◽  
Monika K. Abramczuk ◽  
Kristina Buac ◽  
Lior Zangi ◽  
Maxine W. Stachel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Heart Field ◽  
First Heart Field

scholarly journals Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
LinXi Cheng ◽  
MingHui Xie ◽  
WeiHua Qiao ◽  
Yu Song ◽  
YanYong Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Pluripotent Stem Cells ◽  
Heart Valves ◽  
Human Pluripotent Stem Cells ◽  
Cardiac Progenitors ◽  
Aortic Endothelial Cells ◽  
Clonogenic Potential ◽  
Tissue Engineered Heart Valves ◽  
Human Aortic Endothelial Cells

AbstractThe cardiac valvular endothelial cells (VECs) are an ideal cell source that could be used for making the valve organoids. However, few studies have been focused on the derivation of this important cell type. Here we describe a two-step chemically defined xeno-free method for generating VEC-like cells from human pluripotent stem cells (hPSCs). HPSCs were specified to KDR+/ISL1+ multipotent cardiac progenitors (CPCs), followed by differentiation into valve endothelial-like cells (VELs) via an intermediate endocardial cushion cell (ECC) type. Mechanistically, administration of TGFb1 and BMP4 may specify VEC fate by activating the NOTCH/WNT signaling pathways and previously unidentified targets such as ATF3 and KLF family of transcription factors. When seeded onto the surface of the de-cellularized porcine aortic valve (DCV) matrix scaffolds, hPSC-derived VELs exhibit superior proliferative and clonogenic potential than the primary VECs and human aortic endothelial cells (HAEC). Our results show that hPSC-derived valvular cells could be efficiently generated from hPSCs, which might be used as seed cells for construction of valve organoids or next generation tissue engineered heart valves.

scholarly journals Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

2020 ◽  
Author(s):  
Lingxi Cheng ◽  
Yu Song ◽  
Yanyong Zhang ◽  
Yingchao Geng ◽  
Weilin Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Mechanistic Study ◽  
Cell Type ◽  
Cardiac Progenitors ◽  
Cell Source ◽  
Clonogenic Potential

Abstract The cardiac valvular endothelial cells (VECs) are an ideal cell source that could be used for making the valve organoids. However, few studies have been focused on the derivation of this important cell type. Here we describe a two-step chemically defined xeno-free method for generating VEC-like cells from human pluripotent stem cells (hPSCs). HPSCs were specified to KDR+/ISL1+ multipotent cardiac progenitors (CPCs), followed by differentiation into valve endothelial-like cells (VELs). Mechanistically, administration of TGFb1 and BMP4 may specify VEC fate by activating the NOTCH/WNT signaling pathways and previously unidentified targets such as ATF3 and KLF family of transcription factors. When seeded onto the surface of the de-cellularized porcine aortic valve (DCV) matrix scaffolds, hPSC-derived VELs exhibit superior proliferative and clonogenic potential than the primary VECs. Our results suggest that hPSC-derived VELs could serve as as a potential platform for the mechanistic study of valvulogenesis, and as starting materials for the construction of the valve organoids.

scholarly journals Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jianhua Zhang ◽  
Ran Tao ◽  
Katherine F. Campbell ◽  
Juliana L. Carvalho ◽  
Edward C. Ruiz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cardiac Fibroblasts ◽  
Human Pluripotent Stem Cells ◽  
Second Heart Field ◽  
Heart Field

scholarly journals Direct Contact with Endoderm-Like Cells Efficiently Induces Cardiac Progenitors from Mouse and Human Pluripotent Stem Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e46413 ◽  
Author(s):  
Hideki Uosaki ◽  
Peter Andersen ◽  
Lincoln T. Shenje ◽  
Laviel Fernandez ◽  
Sofie Lindgren Christiansen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Direct Contact ◽  
Human Pluripotent Stem Cells ◽  
Cardiac Progenitors

scholarly journals Functional Cardiac Fibroblasts Derived from Human Pluripotent Stem Cells via Second Heart Field Progenitors

2018 ◽  
Vol 124 ◽  
pp. 83 ◽  
Author(s):  
Jianhua Zhang ◽  
Ran Tao ◽  
Katherine Campbell ◽  
Juliana Carvalho ◽  
Edward Ruiz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cardiac Fibroblasts ◽  
Human Pluripotent Stem Cells ◽  
Second Heart Field ◽  
Heart Field

Differentiation of human pluripotent stem cells into airway epithelial cells

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
S Ulrich ◽  
S Weinreich ◽  
R Haller ◽  
S Menke ◽  
R Olmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial
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