scholarly journals Simulated Microgravity and 3D Culture Enhance Induction, Viability, Proliferation and Differentiation of Cardiac Progenitors from Human Pluripotent Stem Cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Rajneesh Jha ◽  
Qingling Wu ◽  
Monalisa Singh ◽  
Marcela K. Preininger ◽  
Pengcheng Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Simulated Microgravity ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Cardiac Progenitors ◽  
Proliferation And Differentiation

scholarly journals Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture

2015 ◽  
Vol 12 (7) ◽  
pp. 671-678 ◽  
Author(s):  
Anca M Paşca ◽  
Steven A Sloan ◽  
Laura E Clarke ◽  
Yuan Tian ◽  
Christopher D Makinson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cortical Neurons ◽  
3D Culture ◽  
Human Pluripotent Stem Cells

scholarly journals Characterization of Phenotypic and Transcriptional Differences in Human Pluripotent Stem Cells under 2D and 3D Culture Conditions

2016 ◽  
Vol 5 (22) ◽  
pp. 2951-2958 ◽  
Author(s):  
Ken-ichiro Kamei ◽  
Yoshie Koyama ◽  
Yumie Tokunaga ◽  
Yasumasa Mashimo ◽  
Momoko Yoshioka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
Culture Conditions ◽  
Human Pluripotent Stem Cells ◽  
2D And 3D

scholarly journals Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells

Cell Reports ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. 537-550 ◽  
Author(s):  
Keiko Muguruma ◽  
Ayaka Nishiyama ◽  
Hideshi Kawakami ◽  
Kouichi Hashimoto ◽  
Yoshiki Sasai
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Self Organization ◽  
Cerebellar Tissue

scholarly journals Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ulf Diekmann ◽  
Hanna Wolling ◽  
Rabea Dettmer ◽  
Isabell Niwolik ◽  
Ortwin Naujok ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Definitive Endoderm

scholarly journals Assessment of the Hematopoietic Differentiation Potential of Human Pluripotent Stem Cells in 2D and 3D Culture Systems

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2858
Author(s):  
German Atzin Mora-Roldan ◽  
Dalia Ramirez-Ramirez ◽  
Rosana Pelayo ◽  
Karlen Gazarian
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Time Course ◽  
Hematopoietic Cells ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Hematopoietic Differentiation ◽  
Differentiation Potential ◽  
2D And 3D

Background. In vitro methods for hematopoietic differentiation of human pluripotent stem cells (hPSC) are a matter of priority for the in-depth research into the mechanisms of early embryogenesis. So-far, published results regarding the generation of hematopoietic cells come from studies using either 2D or 3D culture formats, hence, it is difficult to discern their particular contribution to the development of the concept of a unique in vitro model in close resemblance to in vivo hematopoiesis. Aim of the study. To assess using the same culture conditions and the same time course, the potential of each of these two formats to support differentiation of human pluripotent stem cells to primitive hematopoiesis without exogenous activation of Wnt signaling. Methods. We used in parallel 2D and 3D formats, the same culture environment and assay methods (flow cytometry, IF, qPCR) to investigate stages of commitment and specification of mesodermal, and hemogenic endothelial cells to CD34 hematopoietic cells and evaluated their clonogenic capacity in a CFU system. Results. We show an adequate formation of mesoderm, an efficient commitment to hemogenic endothelium, a higher number of CD34 hematopoietic cells, and colony-forming capacity potential only in the 3D format-supported differentiation. Conclusions. This study shows that the 3D but not the 2D format ensures the induction and realization by endogenous mechanisms of human pluripotent stem cells’ intrinsic differentiation program to primitive hematopoietic cells. We propose that the 3D format provides an adequate level of upregulation of the endogenous Wnt/β-catenin signaling.

scholarly journals Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
LinXi Cheng ◽  
MingHui Xie ◽  
WeiHua Qiao ◽  
Yu Song ◽  
YanYong Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Pluripotent Stem Cells ◽  
Heart Valves ◽  
Human Pluripotent Stem Cells ◽  
Cardiac Progenitors ◽  
Aortic Endothelial Cells ◽  
Clonogenic Potential ◽  
Tissue Engineered Heart Valves ◽  
Human Aortic Endothelial Cells

AbstractThe cardiac valvular endothelial cells (VECs) are an ideal cell source that could be used for making the valve organoids. However, few studies have been focused on the derivation of this important cell type. Here we describe a two-step chemically defined xeno-free method for generating VEC-like cells from human pluripotent stem cells (hPSCs). HPSCs were specified to KDR+/ISL1+ multipotent cardiac progenitors (CPCs), followed by differentiation into valve endothelial-like cells (VELs) via an intermediate endocardial cushion cell (ECC) type. Mechanistically, administration of TGFb1 and BMP4 may specify VEC fate by activating the NOTCH/WNT signaling pathways and previously unidentified targets such as ATF3 and KLF family of transcription factors. When seeded onto the surface of the de-cellularized porcine aortic valve (DCV) matrix scaffolds, hPSC-derived VELs exhibit superior proliferative and clonogenic potential than the primary VECs and human aortic endothelial cells (HAEC). Our results show that hPSC-derived valvular cells could be efficiently generated from hPSCs, which might be used as seed cells for construction of valve organoids or next generation tissue engineered heart valves.

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