scholarly journals Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes

10.3791/57135 ◽  
2018 ◽  
Author(s):  
Chao Tian ◽  
Wei Zhang ◽  
Van Phuc Nguyen ◽  
Xueding Wang ◽  
Yannis M. Paulus
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Photoacoustic Microscopy ◽  
Dual Modality

scholarly journals Resolution-matched reflection mode photoacoustic microscopy and optical coherence tomography dual modality system

2020 ◽  
Vol 19 ◽  
pp. 100188
Author(s):  
Xiaoyi Zhu ◽  
Zhiyu Huang ◽  
Ziyuan Li ◽  
Wenzhao Li ◽  
Xi Liu ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Photoacoustic Microscopy ◽  
Reflection Mode ◽  
Dual Modality

scholarly journals High Resolution Multimodal Photoacoustic Microscopy and Optical Coherence Tomography Visualization of Choroidal Vascular Occlusion

2020 ◽  
Vol 21 (18) ◽  
pp. 6508
Author(s):  
Van Phuc Nguyen ◽  
Yanxiu Li ◽  
Jessica Henry ◽  
Wei Zhang ◽  
Xueding Wang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Laser Energy ◽  
Vascular Occlusion ◽  
Two Dimensions ◽  
Fundus Photography ◽  
Optical Coherence ◽  
Photoacoustic Microscopy ◽  
Biological Studies ◽  
Multiple Imaging

Photoacoustic microscopy is a novel, non-ionizing, non-invasive imaging technology that evaluates tissue absorption of short-pulsed light through the sound waves emitted by the tissue and has numerous biomedical applications. In this study, a custom-built multimodal imaging system, including photoacoustic microscopy (PAM) and optical coherence tomography (OCT), has been developed to evaluate choroidal vascular occlusion (CVO). CVO was performed on three living rabbits using laser photocoagulation. Longitudinal imaging of CVO was obtained using multiple imaging tools such as color fundus photography, fluorescein angiography, indocyanine green angiography (ICGA), OCT, and PAM. PAM images were acquired at different wavelengths, ranging from 532 to 700 nm. The results demonstrate that the CVO was clearly observed on PAM in both two dimensions (2D) and 3D with high resolution longitudinally over 28 days. In addition, the location and margin of the CVO were distinguished from the surrounding choroidal vasculature after the injection of ICG contrast agent. PAM imaging was achieved using a laser energy of approximately 80 nJ, which is about half of the American National Standards Institute safety limit. The proposed imaging technique may provide a potential tool for the evaluation of different chorioretinal vascular disease pathogeneses and other biological studies.

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