scholarly journals Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

10.3791/56904 ◽  
2018 ◽  
Author(s):  
Shelby Shrigley ◽  
Karolina Pircs ◽  
Roger A. Barker ◽  
Malin Parmar ◽  
Janelle Drouin-Ouellet
Keyword(s):  
Skin Fibroblasts ◽  
High Yield ◽  
Human Adult ◽  
Induced Neurons ◽  
Adult Skin

Generation of Fluorescently Labeled Cell Lines, C3A Hepatoma Cells, and Human Adult Skin Fibroblasts to Study Coculture Models

2013 ◽  
Vol 37 (7) ◽  
pp. E123-E130 ◽  
Author(s):  
Anna Samluk ◽  
Karolina Ewa Zakrzewska ◽  
Krzysztof Dariusz Pluta
Keyword(s):  
Cell Lines ◽  
Hepatoma Cells ◽  
Skin Fibroblasts ◽  
Human Adult ◽  
Adult Skin

scholarly journals Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

10.3791/53880 ◽  
2016 ◽  
Author(s):  
Tiziana A.L. Brevini ◽  
Georgia Pennarossa ◽  
Sara Maffei ◽  
Alessandro Zenobi ◽  
Fulvio Gandolfi
Keyword(s):  
Skin Fibroblasts ◽  
Simple Method ◽  
Insulin Secreting Cells ◽  
Epigenetic Conversion ◽  
Adult Skin

High yield preparation of isolated human adult hepatocytes by enzymatic perfusion of the liver

1982 ◽  
Vol 6 (6) ◽  
pp. 625-628 ◽  
Author(s):  
C GUGUENGUILLOUZO ◽  
J CAMPION ◽  
P BRISSOT ◽  
D GLAISE ◽  
B LAUNOIS ◽  
...  
Keyword(s):  
High Yield ◽  
Human Adult

scholarly journals 270 Phenotypic plasticity of melanocytes derived from human adult skin

2021 ◽  
Vol 141 (10) ◽  
pp. S195
Author(s):  
D.L. Vidacs ◽  
Z. Veréb ◽  
R. Bozó ◽  
L.B. Flink ◽  
H. Polyánka ◽  
...  
Keyword(s):  
Phenotypic Plasticity ◽  
Human Adult ◽  
Adult Skin

Inter- and intra-site heterogeneity in the expression of fetal-like phenotypic characteristics by gingival fibroblasts: potential significance for wound healing

1994 ◽  
Vol 107 (5) ◽  
pp. 1333-1346 ◽  
Author(s):  
C.R. Irwin ◽  
M. Picardo ◽  
I. Ellis ◽  
P. Sloan ◽  
A. Grey ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Fibroblasts ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Early Passage ◽  
Subsequent Work ◽  
Skin Cells ◽  
Fetal Fibroblasts ◽  
Adult Skin ◽  
Migratory Phenotype

We have previously reported that fetal and adult skin fibroblasts display distinctive migratory phenotypes on 3-D collagen substrata and that these behavioural characteristics may be quantified by a function defined as the cell density migration index (CDMI). Subsequent work indicated that this difference in migratory phenotype was due to the production by fetal fibroblasts of a migration stimulating factor (MSF) that is not produced by normal adult skin fibroblasts. We now present data indicating that: (a) unselected fibroblasts obtained from 14/14 (100%) of adult gingival explants expressed fetal-like CDMI values compared to only 1/10 (10%) of similarly explanted paired skin cells; (b) 12/12 (100%) of these gingival fibroblast lines also produced detectable quantities of MSF compared to 0/9 (0%) of the tested skin cells; (c) by microdissection studies, gingival fibroblasts obtained from different anatomical microdomains consisted of behaviourally distinct subpopulations, with cells derived from the papillary tips (PAP fibroblasts) displaying fetal-like CDMI values and persistent MSF production, whilst cells obtained from the deeper reticular tissue (RET fibroblasts) were adult-like with respect to these two criteria; (d) PAP fibroblasts were also smaller and achieved higher saturation cell densities compared to paired RET cells; (e) PAP fibroblasts passaged in vitro underwent a fetal-to-adult phenotypic transition characterized by the adoption of various RET cell characteristics, including the acquisition of CDMI values falling within the adult range and cessation in MSF production; and (f) early passage PAP fibroblasts incubated in the presence of an affinity-purified anti-MSF rabbit polyclonal antibody were induced to alter their migratory phenotype and exhibited CDMI values falling within the adult range. Statistical analysis indicated a highly significant correlation between the expression of a fetal-like CDMI and production of MSF (P < 0.00001, using the Fisher exact contingency test). Taken together, these observations suggest that the production of MSF by PAP fibroblasts is responsible for their characteristically fetal-like migratory behaviour. The existence of such inter- and intra-site phenotypic heterogeneity in populations of skin and gingival fibroblasts is discussed in the context of fibroblast lineage relationships and the possible contribution of persistently fetal-like fibroblast subpopulations to connective tissue function in wound healing.

VERGLEICHENDE UNTERSUCHUNGEN ÜBER DEN STOFFWECHSEL VON [4-14C] PROGESTERON IN DER FETALEN HAUT UND IN DER HAUT DES ERWACHSENEN

1972 ◽  
Vol 70 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Harald Mickan
Keyword(s):  
Experimental Conditions ◽  
Human Adult ◽  
Adult Skin ◽  
Vergleichende Untersuchungen

ABSTRACT The metabolism of [4-14C] progesterone in human adult and foetal skin was investigated in vitro under identical experimental conditions. 5α-Pregnanedione and 20α-dihydroprogesterone were isolated and identified in all the experiments. In addition 3β-hydroxy-5α-pregnan-20-one, 6β-hydroxyprogesterone and tentatively 6β-hydroxy-20α-dihydroprogesterone could be identified in the experiments with foetal skin. 5α-Reductase was shown to be most active in both foetal and adult skin. 5β-saturated products were not detectable. These experiments demonstrate a significant capability of foetal skin in the metabolism of progesterone.

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