scholarly journals Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

10.3791/56026 ◽  
2017 ◽  
Author(s):  
Douglas K. Reilly ◽  
Daniel E. Lawler ◽  
Dirk R. Albrecht ◽  
Jagan Srinivasan
Keyword(s):  
Neuronal Activity ◽  
C Elegans

scholarly journals A Putative Cation Channel, NCA-1, and a Novel Protein, UNC-80, Transmit Neuronal Activity in C. elegans

PLoS Biology ◽  
2008 ◽  
Vol 6 (3) ◽  
pp. e55 ◽  
Author(s):  
Edward Yeh ◽  
Sharon Ng ◽  
Mi Zhang ◽  
Magali Bouhours ◽  
Ying Wang ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Cation Channel ◽  
C Elegans ◽  
Novel Protein

scholarly journals Serotonin differentially modulates Ca2+ transients and depolarization in a C. elegans nociceptor

2015 ◽  
Vol 113 (4) ◽  
pp. 1041-1050 ◽  
Author(s):  
Jeffrey A. Zahratka ◽  
Paul D. E. Williams ◽  
Philip J. Summers ◽  
Richard W. Komuniecki ◽  
Bruce A. Bamber
Keyword(s):  
Steady State ◽  
Neuronal Activity ◽  
Neuronal Excitability ◽  
Cell Soma ◽  
Voltage Gated ◽  
C Elegans ◽  
Escape Responses

Monoamines and neuropeptides modulate neuronal excitability and synaptic strengths, shaping circuit activity to optimize behavioral output. In C. elegans, a pair of bipolar polymodal nociceptors, the ASHs, sense 1-octanol to initiate escape responses. In the present study, 1-octanol stimulated large increases in ASH Ca2+, mediated by L-type voltage-gated Ca2+ channels (VGCCs) in the cell soma and L-plus P/Q-type VGCCs in the axon, which were further amplified by Ca2+ released from intracellular stores. Importantly, 1-octanol-dependent aversive responses were not inhibited by reducing ASH L-VGCC activity genetically or pharmacologically. Serotonin, an enhancer of 1-octanol avoidance, potentiated 1-octanol-dependent ASH depolarization measured electrophysiologically, but surprisingly, decreased the ASH somal Ca2+ transients. These results suggest that ASH somal Ca2+ transient amplitudes may not always be predictive of neuronal depolarization and synaptic output. Therefore, although increases in steady-state Ca2+ can reliably indicate when neurons become active, quantitative relationships between Ca2+ transient amplitudes and neuronal activity may not be as straightforward as previously anticipated.

scholarly journals Long-term activity drives dendritic branch elaboration of a C. elegans sensory neuron

2019 ◽  
Author(s):  
Jesse A Cohn ◽  
Elizabeth R Cebul ◽  
Giulio Valperga ◽  
Mario de Bono ◽  
Maxwell G Heiman ◽  
...  
Keyword(s):  
Sensory Neuron ◽  
Neuronal Activity ◽  
Morphological Changes ◽  
Model Organism ◽  
Neuronal Morphology ◽  
Sensory Transduction ◽  
Central Component ◽  
C Elegans ◽  
Activity Dependent

ABSTRACTNeuronal activity often leads to alterations in gene expression and cellular architecture. The nematode Caenorhabditis elegans, owing to its compact translucent nervous system, is a powerful system in which to study conserved aspects of the development and plasticity of neuronal morphology. Here we focus on one sensory neuron in the worm, termed URX, which senses oxygen and signals tonically proportional to environmental oxygen. Previous studies have reported that URX has variable branched endings at its dendritic sensory tip. By controlling oxygen levels and analyzing mutants, we found that these branched endings grow over time as a consequence of neuronal activity. Furthermore, we observed that the branches contain microtubules, but do not appear to harbor the guanylyl cyclase GCY-35, a central component of the oxygen sensory transduction pathway. Interestingly, we found that although URX dendritic tips grow branches in response to long-term activity, the degree of branch elaboration does not correlate with oxygen sensitivity at the cellular or the behavioral level. Given the strengths of C. elegans as a model organism, URX may serve as a potent system for uncovering genes and mechanisms involved in activity-dependent morphological changes in neurons.

scholarly journals Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 1116-1127 ◽  
Author(s):  
Adam L. Nekimken ◽  
Holger Fehlauer ◽  
Anna A. Kim ◽  
Sandra N. Manosalvas-Kjono ◽  
Purim Ladpli ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Neuronal Activity ◽  
High Resolution Imaging ◽  
C Elegans ◽  
Resolution Imaging ◽  
Simultaneous Immobilization ◽  
Stimulation Of

A new microfluidic tool for simultaneous immobilization, force delivery and high resolution imaging of neuronal activity in living Caenorhabditis elegans.

scholarly journals Eukaryotic initiation factor EIF-3.G augments mRNA translation efficiency to regulate neuronal activity

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Stephen M Blazie ◽  
Seika Takayanagi-Kiya ◽  
Katherine M McCulloch ◽  
Yishi Jin
Keyword(s):  
Neuronal Activity ◽  
Rna Binding ◽  
Mrna Translation ◽  
Initiation Factor ◽  
Translation Efficiency ◽  
Dependent Manner ◽  
C Elegans ◽  
Protein Levels ◽  
Neuronal Protein

The translation initiation complex eIF3 imparts specialized functions to regulate protein expression. However, understanding of eIF3 activities in neurons remains limited despite widespread dysregulation of eIF3 subunits in neurological disorders. Here, we report a selective role of the C. elegans RNA-binding subunit EIF-3.G in shaping the neuronal protein landscape. We identify a missense mutation in the conserved Zinc-Finger (ZF) of EIF-3.G that acts in a gain-of-function manner to dampen neuronal hyperexcitation. Using neuron type-specific seCLIP, we systematically mapped EIF-3.G-mRNA interactions and identified EIF-3.G occupancy on GC-rich 5′UTRs of a select set of mRNAs enriched in activity-dependent functions. We demonstrate that the ZF mutation in EIF-3.G alters translation in a 5′UTR dependent manner. Our study reveals an in vivo mechanism for eIF3 in governing neuronal protein levels to control neuronal activity states and offers insights into how eIF3 dysregulation contributes to neuronal disorders.

scholarly journals Collapse of Global Neuronal States in Caenorhabditis elegans under Isoflurane Anesthesia

2020 ◽  
Vol 133 (1) ◽  
pp. 133-144 ◽  
Author(s):  
Mehraj R. Awal ◽  
Gregory S. Wirak ◽  
Christopher V. Gabel ◽  
Christopher W. Connor
Keyword(s):  
Principal Component Analysis ◽  
Caenorhabditis Elegans ◽  
Neuronal Activity ◽  
High Frequency ◽  
Dynamic Range ◽  
Functional Organization ◽  
Principal Component ◽  
Component Analysis ◽  
C Elegans ◽  
Light Sheet Microscopy

Background A comprehensive understanding of how anesthetics facilitate a reversible collapse of system-wide neuronal function requires measurement of neuronal activity with single-cell resolution. Multineuron recording was performed in Caenorhabditis elegans to measure neuronal activity at varying depths of anesthesia. The authors hypothesized that anesthesia is characterized by dyssynchrony between neurons resulting in a collapse of organized system states. Methods Using light-sheet microscopy and transgenic expression of the calcium-sensitive fluorophore GCaMP6s, a majority of neurons (n = 120) in the C. elegans head were simultaneously imaged in vivo and neuronal activity was measured. Neural activity and system-wide dynamics were compared in 10 animals, progressively dosed at 0%, 4%, and 8% isoflurane. System-wide neuronal activity was analyzed using principal component analysis. Results Unanesthetized animals display distinct global neuronal states that are reflected in a high degree of correlation (R = 0.196 ± 0.070) between neurons and low-frequency, large-amplitude neuronal dynamics. At 4% isoflurane, the average correlation between neurons is significantly diminished (R = 0.026 ± 0.010; P < 0.0001 vs. unanesthetized) and neuron dynamics shift toward higher frequencies but with smaller dynamic range. At 8% isoflurane, interneuronal correlations indicate that neuronal activity remains uncoordinated (R = 0.053 ± 0.029; P < 0.0001 vs. unanesthetized) with high-frequency dynamics that are even further restricted. Principal component analysis of unanesthetized neuronal activity reveals distinct structure corresponding to known behavioral states. At 4% and 8% isoflurane this structure is lost and replaced with randomized dynamics, as quantified by the percentage of total ensemble variance captured by the first three principal components. In unanesthetized worms, this captured variance is high (88.9 ± 5.4%), reflecting a highly organized system, falling significantly at 4% and 8% isoflurane (57.9 ± 11.2%, P < 0.0001 vs. unanesthetized, and 76.0 ± 7.9%, P < 0.001 vs. unanesthetized, respectively) and corresponding to increased randomization and collapse of system-wide organization. Conclusions Anesthesia with isoflurane in C. elegans corresponds to high-frequency randomization of individual neuron activity, loss of coordination between neurons, and a collapse of system-wide functional organization. Editor’s Perspective What We Already Know about This Topic What This Article Tells Us That Is New

scholarly journals A mutation in a CLC anion channel alters serotonergic neuronal activity in C. elegans

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Robyn Branicky ◽  
Hiroaki Miyazaki ◽  
Kevin Strange ◽  
William Schafer
Keyword(s):  
Neuronal Activity ◽  
Anion Channel ◽  
C Elegans

scholarly journals A programmable platform for sub-second multichemical dynamic stimulation and neuronal functional imaging inC. elegans

Lab on a Chip ◽  
2018 ◽  
Vol 18 (3) ◽  
pp. 505-513 ◽  
Author(s):  
T. Rouse ◽  
G. Aubry ◽  
Y. Cho ◽  
M. Zimmer ◽  
H. Lu
Keyword(s):  
Neuronal Activity ◽  
Functional Imaging ◽  
Microfluidic Platform ◽  
C Elegans ◽  
Dynamic Stimulation

This microfluidic platform enables monitoring neuronal activity ofC. elegansin response to dynamic multichemical cues.

scholarly journals The NCA-1 and NCA-2 ion channels function downstream of Gq and Rho to regulate locomotion in C. elegans

2016 ◽  
Author(s):  
Irini Topalidou ◽  
Pin-An Chen ◽  
Kirsten Cooper ◽  
Shigeki Watanabe ◽  
Erik M. Jorgensen ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Nucleotide Exchange ◽  
Loss Of Function ◽  
C Elegans ◽  
Activating Mutations ◽  
Epistasis Analysis ◽  
Guanine Nucleotide Exchange ◽  
Nucleotide Exchange Factor ◽  
Forward Genetic Screens ◽  
Genetic Epistasis

AbstractThe heterotrimeric G protein Gq positively regulates neuronal activity and synaptic transmission. Previously, the Rho guanine nucleotide exchange factor Trio was identified as a direct effector of Gq that acts in parallel to the canonical Gq effector phospholipase C. Here we examine how Trio and Rho act to stimulate neuronal activity downstream of Gq in the nematode Caenorhabditis elegans. Through two forward genetic screens, we identify the cation channels NCA-1 and NCA-2, orthologs of mammalian NALCN, as downstream targets of the Gq/Rho pathway. By performing genetic epistasis analysis using dominant activating mutations and recessive loss-of-function mutations in the members of this pathway, we show that NCA-1 and NCA-2 act downstream of Gq in a linear pathway. Through cell-specific rescue experiments, we show that function of these channels in head acetylcholine neurons is sufficient for normal locomotion in C. elegans. Our results suggest that NCA-1 and NCA-2 are physiologically relevant targets of neuronal Gq-Rho signaling in C. elegans.

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