scholarly journals A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

10.3791/55802 ◽  
2017 ◽  
Author(s):  
Mohamed Salem ◽  
Michal Bernach ◽  
Krzysztof Bajdzienko ◽  
Patrick Giavalisco
Keyword(s):  
Extraction Method ◽  
Comprehensive Analysis ◽  
Single Sample

scholarly journals An improved extraction method enables the comprehensive analysis of lipids, proteins, metabolites and phytohormones from a single sample of leaf tissue under water‐deficit stress

2020 ◽  
Vol 103 (4) ◽  
pp. 1614-1632 ◽  
Author(s):  
Mohamed A. Salem ◽  
Takuya Yoshida ◽  
Leonardo Perez de Souza ◽  
Saleh Alseekh ◽  
Krzysztof Bajdzienko ◽  
...  
Keyword(s):  
Water Deficit ◽  
Extraction Method ◽  
Leaf Tissue ◽  
Comprehensive Analysis ◽  
Single Sample ◽  
Water Deficit Stress

scholarly journals An apoplastic fluid extraction method for the characterization of grapevine leaves proteome and metabolome from a single sample

2020 ◽  
Author(s):  
Joana Figueiredo ◽  
Ana Rita Cavaco ◽  
Leonor Guerra‐Guimarães ◽  
Céline Leclercq ◽  
Jenny Renaut ◽  
...  
Keyword(s):  
Extraction Method ◽  
Single Sample ◽  
Fluid Extraction ◽  
Grapevine Leaves ◽  
Apoplastic Fluid

Comprehensive analysis of gluten in processed foods using a new extraction method and a competitive ELISA based on the R5 antibody

Talanta ◽  
2012 ◽  
Vol 91 ◽  
pp. 33-40 ◽  
Author(s):  
María C. Mena ◽  
Manuel Lombardía ◽  
Alberto Hernando ◽  
Enrique Méndez ◽  
Juan P. Albar
Keyword(s):  
Extraction Method ◽  
Competitive Elisa ◽  
Comprehensive Analysis ◽  
Processed Foods

Response surface methodology to optimise the heat-assisted aqueous extraction of phenolic compounds from coffee parchment and their comprehensive analysis

2019 ◽  
Vol 10 (8) ◽  
pp. 4739-4750 ◽  
Author(s):  
Yolanda Aguilera ◽  
Miguel Rebollo-Hernanz ◽  
Silvia Cañas ◽  
Diego Taladrid ◽  
María A. Martín-Cabrejas
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Extraction Method ◽  
Comprehensive Analysis ◽  
Aqueous Extraction

A sustainable aqueous extraction method was developed using a response surface methodology optimisation for the recovery of coffee parchment phenolics.

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

High throughput semi-automated extraction method for the creation of a collection from microbial fermentations of fungi and Actinomycetes

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
JR Tormo ◽  
N Tabanera ◽  
D Conway ◽  
P Ramos ◽  
A Redondo ◽  
...  
Keyword(s):  
High Throughput ◽  
Extraction Method ◽  
Automated Extraction ◽  
The Creation

A Simple Enzyme-Immunoassay (EIA) Test for Factor VIII-Related Antigen (VIIIAGN)

1979 ◽  
Vol 42 (03) ◽  
pp. 848-854 ◽  
Author(s):  
Paul M Ness ◽  
Herbert A Perkins
Keyword(s):  
Factor Viii ◽  
Enzyme Immunoassay ◽  
Hemophilia A ◽  
Single Sample ◽  
Healthy Controls ◽  
Von Willebrand's Disease ◽  
Factor Viii Related Antigen ◽  
Von Willebrand’S Disease ◽  
Increased Sensitivity

SummaryAn enzyme immunoassay (EIA) system has been developed to measure factor VIII- related antigen (VIIIAGN). This assay gives similar results to the commonly used Laurell electroimmunodiffusion (EID) assay for VIIIAGN as shown by comparison of both techniques with samples from healthy controls, patients with hemophilia A, and patients with von Willebrand’s disease. The assay also has a greater precision than the EID technique as demonstrated by multiple assays of aliquots of a single sample. The use of this EIA test for VIIIAGN is simple and employs inexpensive reagents and equipment. The use of expensive antisera is minimized. EIA for VIIIAGN has the advantage of increased sensitivity compared to Laurell EIA.

Sozial-räumliche Dynamiken der Agrartreibstoffe.

2016 ◽  
Vol 46 (184) ◽  
pp. 423-440 ◽  
Author(s):  
Kristina Dietz ◽  
Bettina Engels ◽  
Oliver Pye
Keyword(s):  
Political Economy ◽  
Capital Flows ◽  
Spatial Dynamics ◽  
Financial Sector ◽  
Comprehensive Analysis ◽  
Spatial Theory ◽  
Global Political Economy

This article explores the spatial dynamics of agrofuels. Building on categories from the field of critical spatial theory, it shows how these categories enable a comprehensive analysis of the spatial dynamics of agrofuels that links the macro-structures of the global political economy to concrete, place-based struggles. Four core socio-spatial dynamics of agrofuel politics are highlighted and applied to empirical findings: territorialization, the financial sector as a new scale of regulation, place-based struggles and transnational spaces of resources and capital flows.

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