scholarly journals Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates

10.3791/55403 ◽  
2017 ◽  
Author(s):  
Viswanath Das ◽  
Tomáš Fürst ◽  
Soňa Gurská ◽  
Petr Džubák ◽  
Marián Hajdúch
Keyword(s):  
Culture Condition ◽  
Multicellular Spheroid ◽  
Spheroid Formation ◽  
Microtiter Plates

Biomaterial Substrate-Mediated Multicellular Spheroid Formation and Their Applications in Tissue Engineering

2017 ◽  
Vol 12 (12) ◽  
pp. 1700064 ◽  
Author(s):  
Ting-Chen Tseng ◽  
Chui-Wei Wong ◽  
Fu-Yu Hsieh ◽  
Shan-hui Hsu
Keyword(s):  
Tissue Engineering ◽  
Multicellular Spheroid ◽  
Spheroid Formation

scholarly journals Tuning microenvironment for multicellular spheroid formation in thermo-responsive anionic microgel scaffolds

2018 ◽  
Vol 106 (11) ◽  
pp. 2899-2909 ◽  
Author(s):  
Xiaolin Cui ◽  
Yusak Hartanto ◽  
Chengtie Wu ◽  
Jingxiu Bi ◽  
Sheng Dai ◽  
...  
Keyword(s):  
Multicellular Spheroid ◽  
Spheroid Formation

Control of polarization and tumoricidal activity of macrophages by multicellular spheroid formation

2018 ◽  
Vol 270 ◽  
pp. 177-183 ◽  
Author(s):  
Yutaro Tanaka ◽  
Makiya Nishikawa ◽  
Yuya Mizukami ◽  
Kosuke Kusamori ◽  
Yuka Ogino ◽  
...  
Keyword(s):  
Multicellular Spheroid ◽  
Spheroid Formation ◽  
Tumoricidal Activity

scholarly journals A novel p70 S6 kinase-microRNA biogenesis axis mediates multicellular spheroid formation in ovarian cancer progression

Oncotarget ◽  
2016 ◽  
Vol 7 (25) ◽  
pp. 38064-38077 ◽  
Author(s):  
Sophia So Ngo Lam ◽  
Carman Ka Man Ip ◽  
Abby Sin Chi Mak ◽  
Alice Sze Tsai Wong
Keyword(s):  
Ovarian Cancer ◽  
Cancer Progression ◽  
Multicellular Spheroid ◽  
Spheroid Formation ◽  
S6 Kinase ◽  
P70 S6 Kinase ◽  
Microrna Biogenesis

Multicellular spheroid formation and evolutionary conserved behaviors of apple snail hemocytes in culture

2013 ◽  
Vol 34 (2) ◽  
pp. 443-453 ◽  
Author(s):  
Juan A. Cueto ◽  
Israel A. Vega ◽  
Alfredo Castro-Vazquez
Keyword(s):  
Multicellular Spheroid ◽  
Apple Snail ◽  
Spheroid Formation

scholarly journals THE EFFECT OF SERUM CONCENTRATION ON THE SPHEROID FORMING ACTIVITY AND CELL GROWTH OF HUMAN MELANOCYTES ON CHITOSAN SURFACE

2006 ◽  
Vol 18 (01) ◽  
pp. 42-46
Author(s):  
SUNG-JAN LIN ◽  
WEN-CHU HSIAO ◽  
CHIH-JUNG HSU ◽  
JAU-SHIUH CHEN ◽  
SHIOU-HWA JEE ◽  
...  
Keyword(s):  
Cell Growth ◽  
Serum Concentration ◽  
Culture System ◽  
Three Dimensional ◽  
Multicellular Spheroid ◽  
Spheroid Formation ◽  
Serum Factors ◽  
Serum Free ◽  
Human Melanocytes ◽  
Coated Surface

We have developed a chitosan-based melanocyte patch to improve the drawbacks encountered in autologous melanocyte transplantation for vitiligo treatment by use of melanocyte suspension. Melanocytes spontaneously grow into three dimensional spheroids on chitosan-coated surface and melanocyte spheroids show a better survival in growth factor and serum-deprived condition. Hence, preculturing melanocytes into spheroids on chitosan surface can provide melanocytes a survival advantage in stringent conditions. Up to date, the mechanism of the melanocyte spheroid formation is not clear. It has been shown that certain factors in serum are crucial for multicellular spheroid formation in cancer cell lines. In this work, we investigate the effect of serum concentration on the melanocyte spheroid formation. The trend for melanocyte spheroid formation is gradually decreased when serum concentration is serially increased from 0% to 20%. Our result shows that melanocyte spheroid formation is not inhibited in the absence of serum and serum deprivation accelerates melanocyte spheroid formation. However, melanocyte growth is greatly reduced when serum is completely removed. Our results suggest that melanocyte spheroid formation on chitosan is not dependent on serum factors. However, the presence of serum contributes to the cell growth on chitosan-coated surface. This result is important in the future development of serum free culture system for spheroidal melanocyte patch.

A microfluidic chip with a U-shaped microstructure array for multicellular spheroid formation, culturing and analysis

2014 ◽  
Vol 6 (1) ◽  
pp. 015009 ◽  
Author(s):  
Chien-Yu Fu ◽  
Sheng-Yang Tseng ◽  
Shih-Mo Yang ◽  
Long Hsu ◽  
Cheng-Hsien Liu ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Multicellular Spheroid ◽  
Spheroid Formation ◽  
Microstructure Array
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