scholarly journals Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

10.3791/54754 ◽  
2016 ◽  
Author(s):  
Tien-Kuan Chen ◽  
Hui-Ting Yang ◽  
Su-Chiung Fang ◽  
Yi-Chen Lien ◽  
Ting-Ting Yang ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Tissue Samples ◽  
Sectioning Method

A New Cadmium Reduction Device for the Microplate Determination of Nitrate in Water, Soil, Plant Tissue, and Physiological Fluids

2011 ◽  
Vol 94 (6) ◽  
pp. 1896-1905 ◽  
Author(s):  
James D Crutchfield ◽  
John H Grove
Keyword(s):  
Plant Tissue ◽  
Low Cost ◽  
Statistical Validation ◽  
Soil Science Society ◽  
Widespread Application ◽  
Tissue Samples ◽  
New Device ◽  
Low Concentrations ◽  
Groundwater Samples ◽  
Interlaboratory Validation

Abstract A reusable catalytic reductor consisting of 96 copperized-cadmium pins attached to a microplate lid was developed to simultaneously reduce nitrate (NO3–) to nitrite (NO2–) in all wells of a standard microplate. The resulting NO2– is analyzed colorimetrically by the Griess reaction using a microplate reader. Nitrate data from groundwater samples analyzed using the new device correlated well with data obtained by ion chromatography (r2 = 0.9959). Soil and plant tissue samples previously analyzed for NO3– in an interlaboratory validation study sponsored by the Soil Science Society of America were also analyzed using the new technique. For the soil sample set, the data are shown to correlate well with the other methods used (r2 = 0.9976). Plant data correlated less well, especially for samples containing low concentrations of NO3–. Reasons for these discrepancies are discussed, and new techniques to increase the accuracy of the analysis are explored. In addition, a method is presented for analyzing NO3– in physiological fluids (blood serum and urine) after matrix modification with Somogyi's reagent. A protocol for statistical validation of data when analyzing samples with complex matrixes is also established. The simplicity, adaptability, and low cost of the device indicate its potential for widespread application.

TISSUE SULFUR LEVELS AND ADDITIONAL SULFUR NEEDS FOR VARIOUS CROPS

1976 ◽  
Vol 56 (3) ◽  
pp. 651-657 ◽  
Author(s):  
UMESH C. GUPTA
Keyword(s):  
Plant Tissue ◽  
Field Survey ◽  
Triticum Aestivum L ◽  
Sulfur Deficiency ◽  
Tissue Samples ◽  
Boot Stage ◽  
P Concentration ◽  
S Deficiency ◽  
Cereal Samples ◽  
Medicago Sativa L

Sulfur deficiency symptoms in barley (Hordeum distichon L. cv. Volla) and wheat (Triticum aestivum L. cv. Opal) were related to < 0.12% S in boot stage tissue (BST) and kernels. The BST and kernel S concentrations of > 0.14% in barley and wheat were in the sufficiency range. The S deficiency and sufficiency levels in the three cuts of alfalfa (Medicago sativa L. cv. Saranac) tissue were 0.15–0.23% and > 0.23%, respectively. For cauliflower, tissue S concentrations of < 0.18% and > 0.19%, respectively, were in the deficiency and sufficiency ranges. Rutabaga (Brassica napobrassica Mill. cv. York) plant tissue S concentrations of < 0.10% were in the deficiency range, while > 0.21% were sufficient. Field survey tissue samples of rutabagas and cauliflower (Brassica oleracea botrytis L. cv. Snowball Y) were in the S sufficiency range, while most alfalfa and a few cereal samples indicated a slight S deficiency problem. The percent P in the BST and kernels of wheat and barley and alfalfa plant tissues was only slightly affected by S applications. Exceptions to this were the BST of barley from the second crop and plant tissue from third-cut alfalfa where S significantly decreased the P concentration of tissue. With added S, the P concentrations increased considerably in rutabaga tissue on both soils.

Elemental Analysis of Plant Tissue by Plasma Emission Spectroscopy: Collaborative Study

1985 ◽  
Vol 68 (3) ◽  
pp. 499-505 ◽  
Author(s):  
Robert A Isaac ◽  
William C Johnson
Keyword(s):  
Plant Tissue ◽  
Emission Spectroscopy ◽  
Collaborative Study ◽  
Plasma Emission ◽  
Tissue Samples ◽  
Coefficients Of Variation ◽  
Aoac Method ◽  
Preparation Errors ◽  
Plasma Emission Spectroscopy

Abstract Fourteen laboratories participated in a collaborative study of 6 homogeneous plant tissue samples to determine the elements P, K, Ca, Mg, Mn, Fe, Al, B, Cu, Zn, and Na by plasma emission spectroscopy. Samples were dry ashed using AOAC method 3.007(a) (13th Ed.). An NBS citrus leaf standard was prepared and a portion of the resulting solution was sent to each collaborator to evaluate sample preparation errors independent of instrument error. Coefficients of variation were better than those obtained in an earlier collaborative study on multielement analysis by spark emission spectroscopy. The plasma emission method has been adopted official first action for determination of P, K, Ca, Mg, Mn, B, Cu, and Zn in plant tissues.

DIGESTION PROCEDURES FOR SIMULTANEOUS AUTOMATED DETERMINATION OF NH4, P, K, CA AND MG IN PLANT MATERIAL

1976 ◽  
Vol 56 (4) ◽  
pp. 425-432 ◽  
Author(s):  
WM. VAN LIEROP
Keyword(s):  
Plant Tissue ◽  
Tissue Samples ◽  
Digestion Technique ◽  
Dry Ashing ◽  
Plant Constituents ◽  
Working Day ◽  
Automated Instruments ◽  
Automated Determination ◽  
Oxidative Agents

The efficacy of several digestion procedures for mineralizing organic matter from plant tissue was compared, and a rapid and precise method suitable for automated determination of NH4, P, K, Ca and Mg in a single digest was selected. A wet-digestion technique using hydrogen peroxide and perchloric acid as oxidative agents in a sulphuric acid medium was deemed the most advantageous among the tested procedures. Appropriate manifolds were designed to determine the sought plant constituents, present in the digests, by automated instruments. Although, recovered ammonium seemed more accurate and precise with the conventional Kjeldahl procedures, mean recovered ammonium was not significantly higher than the suggested method, when the latter was corrected for oxidized ammonium. Further, significantly larger quantities of K, Ca and Mg were recovered compared to the dry-ashing method, though the differences were small. The suggested procedure was verified with a plant-tissue standard and fortified samples. Further, it has been used to digest and analyze over 7,000 plant tissue samples and a number of manure samples without any problems. Two operators can weigh, digest and analyze 100 samples in an average working day without difficulty. An additional operator can, however, quicken the pace and double the number of daily samples.

scholarly journals cDNA microarray analysis of small plant tissue samples using a cDNA tag target amplification protocol

2001 ◽  
Vol 25 (5) ◽  
pp. 585-591 ◽  
Author(s):  
Magnus Hertzberg ◽  
Maria Sievertzon ◽  
Henrik Aspeborg ◽  
Peter Nilsson ◽  
Göran Sandberg ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Cdna Microarray ◽  
Plant Tissue ◽  
Cdna Microarray Analysis ◽  
Tissue Samples ◽  
Small Plant ◽  
Amplification Protocol
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