scholarly journals Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

10.3791/54718 ◽  
2016 ◽  
Author(s):  
Laura Ordovás ◽  
Ruben Boon ◽  
Mariaelena Pistoni ◽  
Yemiao Chen ◽  
Rangarajan Sambathkumar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Cassette Exchange ◽  
Efficient Generation

scholarly journals Rapid and Efficient Generation of Functional Motor Neurons From Human Pluripotent Stem Cells Using Gene Delivered Transcription Factor Codes

2011 ◽  
Vol 19 (10) ◽  
pp. 1905-1912 ◽  
Author(s):  
Mark E Hester ◽  
Matthew J Murtha ◽  
SungWon Song ◽  
Meghan Rao ◽  
Carlos J Miranda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Motor Neurons ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Efficient Generation

scholarly journals Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro

2018 ◽  
Vol 22 (5) ◽  
pp. 684-697.e9 ◽  
Author(s):  
Anindita Sarkar ◽  
Arianna Mei ◽  
Apua C.M. Paquola ◽  
Shani Stern ◽  
Cedric Bardy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Efficient Generation ◽  
Ca3 Neurons

scholarly journals Efficient generation of lung and airway epithelial cells from human pluripotent stem cells

2013 ◽  
Vol 32 (1) ◽  
pp. 84-91 ◽  
Author(s):  
Sarah X L Huang ◽  
Mohammad Naimul Islam ◽  
John O'Neill ◽  
Zheng Hu ◽  
Yong-Guang Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial ◽  
Efficient Generation

scholarly journals A Step-by-Step Refined Strategy for Highly Efficient Generation of Neural Progenitors and Motor Neurons from Human Pluripotent Stem Cells

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3087
Author(s):  
Jie Ren ◽  
Chaoyi Li ◽  
Mengfei Zhang ◽  
Huakun Wang ◽  
Yali Xie ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Motor Neurons ◽  
Neural Development ◽  
Pluripotent Stem Cells ◽  
Disease Modeling ◽  
Human Pluripotent Stem Cells ◽  
Highly Efficient ◽  
Efficient Generation ◽  
Human Neurons

Limited access to human neurons, especially motor neurons (MNs), was a major challenge for studying neurobiology and neurological diseases. Human pluripotent stem cells (hPSCs) could be induced as neural progenitor cells (NPCs) and further multiple neural subtypes, which provide excellent cellular sources for studying neural development, cell therapy, disease modeling and drug screening. It is thus important to establish robust and highly efficient methods of neural differentiation. Enormous efforts have been dedicated to dissecting key signalings during neural commitment and accordingly establishing reliable differentiation protocols. In this study, we refined a step-by-step strategy for rapid differentiation of hPSCs towards NPCs within merely 18 days, combining the adherent and neurosphere-floating methods, as well as highly efficient generation (~90%) of MNs from NPCs by introducing refined sets of transcription factors for around 21 days. This strategy made use of, and compared, retinoic acid (RA) induction and dual-SMAD pathway inhibition, respectively, for neural induction. Both methods could give rise to highly efficient and complete generation of preservable NPCs, but with different regional identities. Given that the generated NPCs can be differentiated into the majority of excitatory and inhibitory neurons, but hardly MNs, we thus further differentiate NPCs towards MNs by overexpressing refined sets of transcription factors, especially by adding human SOX11, whilst improving a series of differentiation conditions to yield mature MNs for good modeling of motor neuron diseases. We thus refined a detailed step-by-step strategy for inducing hPSCs towards long-term preservable NPCs, and further specified MNs based on the NPC platform.

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