scholarly journals Efficient generation of endothelial cells from human pluripotent stem cells and characterization of their functional properties

2015 ◽  
Vol 104 (3) ◽  
pp. 678-687 ◽  
Author(s):  
Wei Song ◽  
Dan S. Kaufman ◽  
Wei Shen
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Functional Properties ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Efficient Generation

scholarly journals Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

2020 ◽  
Author(s):  
Lingxi Cheng ◽  
Yu Song ◽  
Yanyong Zhang ◽  
Yingchao Geng ◽  
Weilin Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Mechanistic Study ◽  
Cell Type ◽  
Cardiac Progenitors ◽  
Cell Source ◽  
Clonogenic Potential

Abstract The cardiac valvular endothelial cells (VECs) are an ideal cell source that could be used for making the valve organoids. However, few studies have been focused on the derivation of this important cell type. Here we describe a two-step chemically defined xeno-free method for generating VEC-like cells from human pluripotent stem cells (hPSCs). HPSCs were specified to KDR+/ISL1+ multipotent cardiac progenitors (CPCs), followed by differentiation into valve endothelial-like cells (VELs). Mechanistically, administration of TGFb1 and BMP4 may specify VEC fate by activating the NOTCH/WNT signaling pathways and previously unidentified targets such as ATF3 and KLF family of transcription factors. When seeded onto the surface of the de-cellularized porcine aortic valve (DCV) matrix scaffolds, hPSC-derived VELs exhibit superior proliferative and clonogenic potential than the primary VECs. Our results suggest that hPSC-derived VELs could serve as as a potential platform for the mechanistic study of valvulogenesis, and as starting materials for the construction of the valve organoids.

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