scholarly journals Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury

10.3791/54700 ◽  
2017 ◽  
Author(s):  
Ravi Alluri ◽  
Hilliard L. Kutscher ◽  
Barbara A. Mullan ◽  
Bruce A. Davidson ◽  
Paul R. Knight
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Gastric Acid ◽  
Murine Model ◽  
Acid Aspiration

scholarly journals Effects of Positive End-expiratory Pressure Titration and Recruitment Maneuver on Lung Inflammation and Hyperinflation in Experimental Acid Aspiration–induced Lung Injury

2012 ◽  
Vol 117 (6) ◽  
pp. 1322-1334 ◽  
Author(s):  
Aline M. Ambrosio ◽  
Rubin Luo ◽  
Denise T. Fantoni ◽  
Claudia Gutierres ◽  
Qin Lu ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Lung Inflammation ◽  
Recruitment Maneuver ◽  
Alveolar Wall ◽  
Arterial Oxygenation ◽  
Positive End Expiratory Pressure ◽  
Acid Aspiration ◽  
Recruitment Maneuvers ◽  
Alveolar Area

Background In acute lung injury positive end-expiratory pressure (PEEP) and recruitment maneuver are proposed to optimize arterial oxygenation. The aim of the study was to evaluate the impact of such a strategy on lung histological inflammation and hyperinflation in pigs with acid aspiration-induced lung injury. Methods Forty-seven pigs were randomly allocated in seven groups: (1) controls spontaneously breathing; (2) without lung injury, PEEP 5 cm H2O; (3) without lung injury, PEEP titration; (4) without lung injury, PEEP titration + recruitment maneuver; (5) with lung injury, PEEP 5 cm H2O; (6) with lung injury, PEEP titration; and (7) with lung injury, PEEP titration + recruitment maneuver. Acute lung injury was induced by intratracheal instillation of hydrochloric acid. PEEP titration was performed by incremental and decremental PEEP from 5 to 20 cm H2O for optimizing arterial oxygenation. Three recruitment maneuvers (pressure of 40 cm H2O maintained for 20 s) were applied to the assigned groups at each PEEP level. Proportion of lung inflammation, hemorrhage, edema, and alveolar wall disruption were recorded on each histological field. Mean alveolar area was measured in the aerated lung regions. Results Acid aspiration increased mean alveolar area and produced alveolar wall disruption, lung edema, alveolar hemorrhage, and lung inflammation. PEEP titration significantly improved arterial oxygenation but simultaneously increased lung inflammation in juxta-diaphragmatic lung regions. Recruitment maneuver during PEEP titration did not induce additional increase in lung inflammation and alveolar hyperinflation. Conclusion In a porcine model of acid aspiration-induced lung injury, PEEP titration aimed at optimizing arterial oxygenation, substantially increased lung inflammation. Recruitment maneuvers further improved arterial oxygenation without additional effects on inflammation and hyperinflation.

scholarly journals Metabolomics Investigation Reveals Metabolite Mediators Associated with Acute Lung Injury and Repair in a Murine Model of Influenza Pneumonia

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Liang Cui ◽  
Dahai Zheng ◽  
Yie Hou Lee ◽  
Tze Khee Chan ◽  
Yadunanda Kumar ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Murine Model ◽  
Influenza Pneumonia ◽  
Injury And Repair

scholarly journals Effect of Long-Term Antiorthostatic Suspension in a Murine Model of Acute Lung Injury

2016 ◽  
Vol 9 (4) ◽  
pp. 332-338
Author(s):  
Tae Young Jang ◽  
Ah-Yeoun Jung ◽  
Young Hyo Kim
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Murine Model

Acute lung injury leads to depression-like symptoms through upregulation of neutrophilic and neuronal NADPH oxidase signaling in a murine model

2017 ◽  
Vol 47 ◽  
pp. 218-226 ◽  
Author(s):  
Ahmed Nadeem ◽  
Nahid Siddiqui ◽  
Naif O. Al-Harbi ◽  
Sabry M. Attia ◽  
Shakir D. AlSharari ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Nadph Oxidase ◽  
Murine Model

scholarly journals Anti-inflammatory effects of triptolide by inhibiting the NF-κB signalling pathway in LPS-induced acute lung injury in a murine model

2014 ◽  
Vol 10 (1) ◽  
pp. 447-452 ◽  
Author(s):  
XIAN WANG ◽  
LEI ZHANG ◽  
WEI DUAN ◽  
BIN LIU ◽  
PING GONG ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Murine Model ◽  
Signalling Pathway ◽  
Anti Inflammatory

scholarly journals Src kinase inhibition with dasatinib impairs neutrophil function and clearance of Escherichia coli infection in a murine model of acute lung injury

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
James G. Macfarlane ◽  
David A. Dorward ◽  
Marie-Hélène Ruchaud-Sparagano ◽  
Jonathan Scott ◽  
Christopher D. Lucas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Murine Model ◽  
Acute Inflammation ◽  
Neutrophil Function ◽  
Mouse Lung ◽  
Human Neutrophils ◽  
Bacterial Clearance ◽  
E Coli

Abstract Background Neutrophils rapidly respond to and clear infection from tissues, but can also induce tissue damage through excessive degranulation, when acute inflammation proceeds unchecked. A number of key neutrophil functions, including adhesion-dependent degranulation, are controlled by src family kinases. Dasatinib is a potent src inhibitor used in treating patients with chronic myeloid leukaemia and treatment-resistant acute lymphoblastic leukaemia. We hypothesized that dasatinib would attenuate acute inflammation by inhibiting neutrophil recruitment, degranulation and endothelial cell injury, without impairing bacterial clearance, in a murine model of bacteria-induced acute lung injury. C57BL/6 mice received intratracheal Escherichia coli, and were treated with intraperitoneal dasatinib or control. Bacterial clearance, lung injury, and markers of neutrophil recruitment and degranulation were measured. Separately, human blood neutrophils were exposed to dasatinib or control, and the effects on a range of neutrophil functions assessed. Results Dasatinib was associated with a dose-dependent significant increase in E. coli in the mouse lung, accompanied by impairment of organ function, reflected in significantly increased protein leak across the alveolar-capillary membrane. However, the number of neutrophils entering the lung was unaffected, suggesting that dasatinib impairs neutrophil function independent of migration. Dasatinib did not cause direct toxicity to human neutrophils, but led to significant reductions in phagocytosis of E. coli, adhesion, chemotaxis, generation of superoxide anion and degranulation of primary and secondary granules. However, no biologically important effect of dasatinib on neutrophil degranulation was observed in mice. Conclusions Contrary to our starting hypothesis, src kinase inhibition with dasatinib had a detrimental effect on bacterial clearance in the mouse lung and therefore does not represent an attractive therapeutic strategy to treat primary infective lung inflammation. Data from human neutrophils suggest that dasatanib has inhibitory effects on a range of neutrophil functions.

Gas Exchange and Histopathology in a 2 Hit Murine Model of Acute Lung Injury (ALI).

2009 ◽  
Author(s):  
F Fichtner ◽  
MT Voelker ◽  
A Hoelman ◽  
T Busch ◽  
M Kasper ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Gas Exchange ◽  
Lung Injury ◽  
Murine Model
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