scholarly journals Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

10.3791/53949 ◽  
2016 ◽  
Author(s):  
Siwei Zhang ◽  
Jingjing Li ◽  
Robert Lea ◽  
Enrique Amaya
Keyword(s):  
Primary Neurogenesis

scholarly journals Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shiv Kumar ◽  
Zobia Umair ◽  
Vijay Kumar ◽  
Santosh Kumar ◽  
Unjoo Lee ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Bmp Signaling ◽  
Promoter Regions ◽  
Occupied Territory ◽  
Neural Genes ◽  
Synergistic Activation ◽  
Direct Binding ◽  
Neural Ectoderm ◽  
Developmental Decision ◽  
Primary Neurogenesis

Abstract Neuroectoderm formation is the first step in development of a proper nervous system for vertebrates. The developmental decision to form a non-neural ectoderm versus a neural one involves the regulation of BMP signaling, first reported many decades ago. However, the precise regulatory mechanism by which this is accomplished has not been fully elucidated, particularly for transcriptional regulation of certain key transcription factors. BMP4 inhibition is a required step in eliciting neuroectoderm from ectoderm and Foxd4l1.1 is one of the earliest neural genes highly expressed in the neuroectoderm and conserved across vertebrates, including humans. In this work, we focused on how Foxd4l1.1 downregulates the neural repressive pathway. Foxd4l1.1 inhibited BMP4/Smad1 signaling and triggered neuroectoderm formation in animal cap explants of Xenopus embryos. Foxd4l1.1 directly bound within the promoter of endogenous neural repressor ventx1.1 and inhibited ventx1.1 transcription. Foxd4l1.1 also physically interacted with Xbra in the nucleus and inhibited Xbra-induced ventx1.1 transcription. In addition, Foxd4l1.1 also reduced nuclear localization of Smad1 to inhibit Smad1-mediated ventx1.1 transcription. Foxd4l1.1 reduced the direct binding of Xbra and Smad1 on ventx1.1 promoter regions to block Xbra/Smad1-induced synergistic activation of ventx1.1 transcription. Collectively, Foxd4l1.1 negatively regulates transcription of a neural repressor ventx1.1 by multiple mechanisms in its exclusively occupied territory of neuroectoderm, and thus leading to primary neurogenesis. In conjunction with the results of our previous findings that ventx1.1 directly represses foxd4l1.1, the reciprocal repression of ventx1.1 and foxd4l1.1 is significant in at least in part specifying the mechanism for the non-neural versus neural ectoderm fate determination in Xenopus embryos.

scholarly journals The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis

2001 ◽  
Vol 107 (1-2) ◽  
pp. 119-131 ◽  
Author(s):  
Alejandra R Paganelli ◽  
Oscar H Ocaña ◽  
Marı́a I Prat ◽  
Paula G Franco ◽  
Silvia L López ◽  
...  
Keyword(s):  
Sonic Hedgehog ◽  
Presenilin 1 ◽  
Related Gene ◽  
Primary Neurogenesis

Primary neurogenesis in Xenopus embryos regulated by a homologue of the Drosophila neurogenic gene Delta

Nature ◽  
1995 ◽  
Vol 375 (6534) ◽  
pp. 761-766 ◽  
Author(s):  
Ajay Chitnis ◽  
Domingos Henrique ◽  
Julian Lewis ◽  
David Ish-Horowicz ◽  
Chris Kintner
Keyword(s):  
Xenopus Embryos ◽  
Primary Neurogenesis

scholarly journals Interaction of Sox1, Sox2, Sox3 and Oct4 during primary neurogenesis

2011 ◽  
Vol 350 (2) ◽  
pp. 429-440 ◽  
Author(s):  
Tenley C. Archer ◽  
Jing Jin ◽  
Elena S. Casey
Keyword(s):  
Primary Neurogenesis

scholarly journals Xebf3 Is a Regulator of Neuronal Differentiation during Primary Neurogenesis in Xenopus

2001 ◽  
Vol 233 (2) ◽  
pp. 495-512 ◽  
Author(s):  
Ombretta Pozzoli ◽  
Alessandro Bosetti ◽  
Laura Croci ◽  
G.Giacomo Consalez ◽  
Monica L. Vetter
Keyword(s):  
Neuronal Differentiation ◽  
Primary Neurogenesis

X-Serrate-1 is involved in primary neurogenesis in Xenopus laevis in a complementary manner with X-Delta-1

2001 ◽  
Vol 211 (8-9) ◽  
pp. 367-376 ◽  
Author(s):  
Tomomi Kiyota ◽  
Hideaki Jono ◽  
Sei Kuriyama ◽  
Kouichi Hasegawa ◽  
Seiji Miyatani ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Primary Neurogenesis
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