scholarly journals Quantifying Fish Swimming Behavior in Response to Acute Exposure of Aqueous Copper Using Computer Assisted Video and Digital Image Analysis

Author(s):  
Robin D. Calfee ◽  
Holly J. Puglis ◽  
Edward E. Little ◽  
William G. Brumbaugh ◽  
Christopher A. Mebane
HortScience ◽  
2004 ◽  
Vol 39 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Mercy A. Olmstead ◽  
Robert Wample ◽  
Stephanie Greene ◽  
Julie Tarara

Traditionally, vegetative cover has been subjectively assessed by visual assessment. However, visual assessment is thought to overestimate percent vegetative cover. Thus, a repeatable method to objectively quantify percent cover is desirable. In two vineyards near Prosser, Wash., the percentage of ground surface covered by up to 15 different cover crops was assessed both visually and by computer-assisted digital image analysis. Quadrats in the cover crop were photographed digitally and the images analyzed with commercially available software. Areas of green vegetation in each image were identified and measured. Weeds in some images were differentiated from the cover crop by user-defined thresholds. Subjective visual estimates of percent vegetative cover were generally higher than those digitally estimated. Values for the visual estimates ranged from 5% to 70% in 1998 (mean = 52.4%) and 7.5% to 55% in 1999 (mean = 30.7%), compared to digital readings ranging from 0.5% to 24% (mean = 11.1%) and 10.3% to 36.6% cover (mean = 20.1%), respectively. The visual assessments had lower coefficients of variability in 1998 (cv 28.1) than the digital image analysis (cv 52.3), but in 1999, the values for the two techniques were similar (cv 41.2 vs. cv 45.7). Despite initial variations between the two methods, the accuracy of digital image analysis for measuring percentage vegetative cover is superior.


2019 ◽  
Vol 20 (18) ◽  
pp. 4580 ◽  
Author(s):  
Kasprzak ◽  
Seraszek-Jaros ◽  
Jagielska ◽  
Helak-Łapaj ◽  
Siodła ◽  
...  

The practical use of knowledge on the diagnostic-prognostic role of polysaccharide components of mucins in colorectal cancer (CRC) has been difficult, due to the number of histochemical (HC) reaction types, as well as lack of standard methods of computer-assisted analysis of tissue expression of these molecules. Using two algorithms of digital image analysis (by application of Image-Pro Premier and our originally designed program Filter HSV), we evaluated the expression of polysaccharides in tissue samples of CRC patients (n = 33), and fragments of normal colorectal tissue from the same patients (control) using periodic acid Schiff reaction (PAS) (neutral mucins) and alcian blue staining (AB) (acidic mucins). Our results indicate lower expression of the PAS+ and AB+ mucins in CRC, as compared to the control samples. The higher expression of PAS+ polysaccharides was detected in flat tumors than in protruded CRC, while higher AB+ mucins expression was a feature of mucinous CRC subtypes. Positive correlation between mutual PAS+ and AB+ expression, as well as correlations with glucose concentration (PAS+ mucins), and hemoglobin level (AB+ mucins) were observed exclusively in unchanged colorectal samples (control). Both algorithms of digital image analysis (smart segmentation and Filter HSV) work properly and can be used interchangeably in daily practice of pathologists, as useful tools of quantitative evaluation of HC reaction in both normal and cancerous tissues.


2012 ◽  
Vol 51 (1) ◽  
pp. 63-73 ◽  
Author(s):  
Lukas Jeker ◽  
Lukas Schmid ◽  
Thomas Meschberger ◽  
Marco Candolfi ◽  
Stefan Pudenz ◽  
...  

2008 ◽  
Vol 86 (11) ◽  
pp. 1603-1609 ◽  
Author(s):  
Nadja Niclauss ◽  
Antonino Sgroi ◽  
Philippe Morel ◽  
Reto Baertschiger ◽  
Mathieu Armanet ◽  
...  

2000 ◽  
Vol 10 (2) ◽  
pp. 7-9
Author(s):  
Yaser Natour ◽  
Christine Sapienza ◽  
Mark Schmalz ◽  
Savita Collins

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