scholarly journals Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

10.3791/50443 ◽  
2013 ◽  
Author(s):  
Catheleyne D'hondt ◽  
Bernard Himpens ◽  
Geert Bultynck
Keyword(s):  
Endothelial Cells ◽  
Wave Propagation ◽  
Mechanical Stimulation ◽  
Calcium Wave ◽  
Corneal Endothelial Cells ◽  
Cell Monolayers

scholarly journals Thrombin Inhibits Intercellular Calcium Wave Propagation in Corneal Endothelial Cells by Modulation of Hemichannels and Gap Junctions

2007 ◽  
Vol 48 (1) ◽  
pp. 120 ◽  
Author(s):  
Catheleyne D’hondt ◽  
Raf Ponsaerts ◽  
Sangly P. Srinivas ◽  
Johan Vereecke ◽  
Bernard Himpens
Keyword(s):  
Endothelial Cells ◽  
Wave Propagation ◽  
Gap Junctions ◽  
Calcium Wave ◽  
Corneal Endothelial Cells

The Myosin II ATPase Inhibitor Blebbistatin Prevents Thrombin-Induced Inhibition of Intercellular Calcium Wave Propagation in Corneal Endothelial Cells

2008 ◽  
Vol 49 (11) ◽  
pp. 4816 ◽  
Author(s):  
Raf Ponsaerts ◽  
Catheleyne D'hondt ◽  
Geert Bultynck ◽  
Sangly P. Srinivas ◽  
Johan Vereecke ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Wave Propagation ◽  
Myosin Ii ◽  
Calcium Wave ◽  
Corneal Endothelial Cells ◽  
Atpase Inhibitor

scholarly journals Calcium Wave Propagation in Networks of Endothelial Cells: Model-based Theoretical and Experimental Study

2012 ◽  
Vol 8 (12) ◽  
pp. e1002847 ◽  
Author(s):  
Juexuan Long ◽  
Michael Junkin ◽  
Pak Kin Wong ◽  
James Hoying ◽  
Pierre Deymier
Keyword(s):  
Experimental Study ◽  
Endothelial Cells ◽  
Wave Propagation ◽  
Calcium Wave ◽  
Model Based

scholarly journals Connexin 40 and ATP-dependent intercellular calcium wave in renal glomerular endothelial cells

2008 ◽  
Vol 294 (6) ◽  
pp. R1769-R1776 ◽  
Author(s):  
Ildikó Toma ◽  
Eric Bansal ◽  
Elliott J. Meer ◽  
Jung Julie Kang ◽  
Sarah L. Vargas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Wave Propagation ◽  
Fold Increase ◽  
Extracellular Atp ◽  
Physical Contact ◽  
Calcium Wave ◽  
Fluorescence Confocal Microscopy ◽  
Glomerular Endothelial Cells ◽  
Connexin 40 ◽  
Voltage Dependent

Endothelial intracellular calcium ([Ca2+]i) plays an important role in the function of the juxtaglomerular vasculature. The present studies aimed to identify the existence and molecular elements of an endothelial calcium wave in cultured glomerular endothelial cells (GENC). GENCs on glass coverslips were loaded with Fluo-4/Fura red, and ratiometric [Ca2+]iimaging was performed using fluorescence confocal microscopy. Mechanical stimulation of a single GENC caused a nine-fold increase in [Ca2+]i, which propagated from cell to cell throughout the monolayer (7.9 ± 0.3 μm/s) in a regenerative manner (without decrement of amplitude, kinetics, and speed) over distances >400 μm. Inhibition of voltage-dependent calcium channels with nifedipine had no effect on the above parameters, but the removal of extracellular calcium reduced Δ[Ca2+]iby 50%. Importantly, the gap junction uncoupler α-glycyrrhetinic acid or knockdown of connexin 40 (Cx40) by transfecting GENCs with Cx40 short interfering RNA (siRNA) almost completely eliminated Δ[Ca2+]iand the calcium wave. Breakdown of extracellular ATP using a scavenger cocktail (apyrase and hexokinase) or nonselective inhibition of purinergic P2 receptors with suramin, had similar blocking effects. Scraping cells off along a line eliminated physical contact between cells but did not effect calcium wave propagation. Using an ATP biosensor technique, we detected a significant elevation in extracellular ATP (Δ = 76 ± 2 μM) during calcium wave propagation, which was abolished by Cx40 siRNA treatment (Δ = 6 ± 1 μM). These studies suggest that connexin 40 hemichannels and extracellular ATP are key molecular elements of the glomerular endothelial calcium wave, which may serve important juxtaglomerular functions.

scholarly journals Adenosine Opposes Thrombin-Induced Inhibition of Intercellular Calcium Wave in Corneal Endothelial Cells

2007 ◽  
Vol 48 (4) ◽  
pp. 1518 ◽  
Author(s):  
Catheleyne D’hondt ◽  
Sangly P. Srinivas ◽  
Johan Vereecke ◽  
Bernard Himpens
Keyword(s):  
Endothelial Cells ◽  
Calcium Wave ◽  
Corneal Endothelial Cells

Culturing Discarded Peripheral Human Corneal Endothelial Cells From the Tissues Deemed for Preloaded DMEK Transplants

Cornea ◽  
2019 ◽  
Vol 38 (9) ◽  
pp. 1175-1181 ◽  
Author(s):  
Mohit Parekh ◽  
Vito Romano ◽  
Alessandro Ruzza ◽  
Stephen B. Kaye ◽  
Diego Ponzin ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells
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