Mechanism of intracellular Ca2+-wave propagation elicited by mechanical stimulation in cultured endothelial CPAE cells and intercellular communication upon mechanical stimulation of CPAE-endothelial cells is mediated by nucleotides

Cell Calcium ◽  
2001 ◽  
Vol 29 (4) ◽  
pp. 288
Keyword(s):  
Endothelial Cells ◽  
Wave Propagation ◽  
Mechanical Stimulation ◽  
Intercellular Communication ◽  
Cpae Cells ◽  
Stimulation Of

scholarly journals Mechanical stimulation and intercellular communication increases intracellular Ca2+ in epithelial cells.

1990 ◽  
Vol 1 (8) ◽  
pp. 585-596 ◽  
Author(s):  
M J Sanderson ◽  
A C Charles ◽  
E R Dirksen
Keyword(s):  
Epithelial Cells ◽  
Respiratory Tract ◽  
Gap Junctions ◽  
Mechanical Stimulation ◽  
Intercellular Communication ◽  
Calcium Concentration ◽  
Ciliated Cells ◽  
Inositol 1,4,5 Trisphosphate ◽  
Ciliated Epithelial Cells ◽  
Stimulation Of

Intercellular communication of epithelial cells was examined by measuring changes in intracellular calcium concentration ([Ca2+]i). Mechanical stimulation of respiratory tract ciliated cells in culture induced a wave of increasing Ca2+ that spread, cell by cell, from the stimulated cell to neighboring cells. The communication of these Ca2+ waves between cells was restricted or blocked by halothane, an anesthetic known to uncouple cells. In the absence of extracellular Ca2+, the mechanically stimulated cell showed no change or a decrease in [Ca2+]i, whereas [Ca2+]i increased in neighboring cells. Iontophoretic injection of inositol 1,4,5-trisphosphate (IP3) evoked a communicated Ca2+ response that was similar to that produced by mechanical stimulation. These results support the hypothesis that IP3 acts as a cellular messenger that mediates communication through gap junctions between ciliated epithelial cells.

Mechanical stimulation of human BON cells: Gαq involvement in 5-HT release

2001 ◽  
Vol 120 (5) ◽  
pp. A83-A83
Author(s):  
M KIM ◽  
N JAVED ◽  
F CHRISTOFI ◽  
H COOKE
Keyword(s):  
Mechanical Stimulation ◽  
Bon Cells ◽  
Stimulation Of

Use of Flexible Materials with a Novel Pressure Driven Equibiaxial Cell Stretching Device for Mechanical Stimulation of Single Mammalian Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
James D. Kubicek ◽  
Stephanie Brelsford ◽  
Philip R. LeDuc
Keyword(s):  
Cell Fate ◽  
Mechanical Stimulation ◽  
Mammalian Cells ◽  
Cellular Response ◽  
Single Cells ◽  
Complex Nature ◽  
Cellular Behavior ◽  
Cell Stretching ◽  
Stimulation Of ◽  
Pressure Driven

AbstractMechanical stimulation of single cells has been shown to affect cellular behavior from the molecular scale to ultimate cell fate including apoptosis and proliferation. In this, the ability to control the spatiotemporal application of force on cells through their extracellular matrix connections is critical to understand the cellular response of mechanotransduction. Here, we develop and utilize a novel pressure-driven equibiaxial cell stretching device (PECS) combined with an elastomeric material to control specifically the mechanical stimulation on single cells. Cells were cultured on silicone membranes coated with molecular matrices and then a uniform pressure was introduced to the opposite surface of the membrane to stretch single cells equibiaxially. This allowed us to apply mechanical deformation to investigate the complex nature of cell shape and structure. These results will enhance our knowledge of cellular and molecular function as well as provide insights into fields including biomechanics, tissue engineering, and drug discovery.

Mechanical stimulation of duck on rice phyto-morphology in rice-duck farming system

2012 ◽  
Vol 20 (6) ◽  
pp. 717-722 ◽  
Author(s):  
Zhao-Xiang HUANG ◽  
Jia-En ZHANG ◽  
Kai-Ming LIANG ◽  
Guo-Ming QUAN ◽  
Ben-Liang ZHAO
Keyword(s):  
Mechanical Stimulation ◽  
Farming System ◽  
Stimulation Of
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