scholarly journals In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

10.3791/2760 ◽  
2012 ◽  
Author(s):  
Dimitrios Davalos ◽  
Katerina Akassoglou
Keyword(s):  
Spinal Cord ◽  
Mouse Spinal Cord ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy

2008 ◽  
Vol 169 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Dimitrios Davalos ◽  
Jae K. Lee ◽  
W. Bryan Smith ◽  
Brendan Brinkman ◽  
Mark H. Ellisman ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Blood Vessels ◽  
In Vivo Imaging ◽  
Mouse Spinal Cord ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals In Vivo Two-Photon Imaging of Neurons and Glia in the Mouse Spinal Cord

2012 ◽  
Vol 2012 (12) ◽  
pp. pdb.prot072264-pdb.prot072264 ◽  
Author(s):  
H. Steffens ◽  
F. Nadrigny ◽  
F. Kirchhoff
Keyword(s):  
Spinal Cord ◽  
Mouse Spinal Cord ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Giovanni Cirillo ◽  
Daniele De Luca ◽  
Michele Papa
Keyword(s):  
Spinal Cord ◽  
Cultured Cells ◽  
Ex Vivo ◽  
Sensory Stimulation ◽  
Widespread Application ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Heart Beating ◽  
Sensory Activity

Astrocytic Ca2+dynamics have been extensively studied inex vivomodels; however, the recent development of two-photon microscopy and astrocyte-specific labeling has allowed the study of Ca2+signaling in living central nervous system. Ca2+waves in astrocytes have been described in cultured cells and slice preparations, but evidence for astrocytic activation during sensory activity is lacking. There are currently few methods to image living spinal cord: breathing and heart-beating artifacts have impeded the widespread application of this technique. We here imaged the living spinal cord by two-photon microscopy in C57BL6/J mice. Through pressurized injection, we specifically loaded spinal astrocytes using the red fluorescent dye sulforhodamine 101 (SR101) and imaged astrocytic Ca2+levels with Oregon-Green BAPTA-1 (OGB). Then, we studied astrocytic Ca2+levels at rest and after right electrical hind paw stimulation. Sensory stimulation significantly increased astrocytic Ca2+levels within the superficial dorsal horn of the spinal cord compared to rest. In conclusion,in vivomorphofunctional imaging of living astrocytes in spinal cord revealed that astrocytes actively participate to sensory stimulation.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

Sensory Transmission is Bi-directionally Modulated by Astrocytic Ca2+ in Barrel Cortex of Behaving Mice

2021 ◽  
Author(s):  
Simeng Gu ◽  
Wei Wang ◽  
Kuan Zhang ◽  
Rou Feng ◽  
Naling Li ◽  
...  
Keyword(s):  
Sensory Input ◽  
Barrel Cortex ◽  
Synaptic Function ◽  
Metabolic Clearance ◽  
Sleep State ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Sensory Transmission

Abstract Different effects of astrocyte during sleep and awake have been extensively studied, especially for metabolic clearance by the glymphatic system, which works during sleep and stops working during waking states. However, how astrocytes contribute to modulation of sensory transmission during sleep and awake animals remain largely unknown. Recent advances in genetically encoded Ca2+ indicators have provided a wealth of information on astrocytic Ca2+, especially in their fine perisynaptic processes, where astrocytic Ca2+ most likely affects the synaptic function. Here we use two-photon microscopy to image astrocytic Ca2+ signaling in freely moving mice trained to run on a wheel in combination with in vivo whole-cell recordings to evaluate the role of astrocytic Ca2+ signaling in different behavior states. We found that there are two kinds of astrocytic Ca2+ signaling: a small long-lasting Ca2+ increase during sleep state and a sharp widespread but short-long-lasting Ca2+ spike when the animal was awake (fluorescence increases were 23.2 ± 14.4% for whisker stimulation at sleep state, compared with 73.3 ± 11.7% for at awake state, paired t-test, p < 0.01). The small Ca2+ transients decreased extracellular K+, hyperpolarized the neurons, and suppressed sensory transmission; while the large Ca2+ wave enhanced sensory input, contributing to reliable sensory transmission in aroused states. Locus coeruleus activation works as a switch between these two kinds of astrocytic Ca2+ elevation. Thus, we show that cortical astrocytes play an important role in processing of sensory input. These two types of events appear to have different pharmacological sources and may play a different role in facilitating the efficacy of sensory transmission.

Quantum Dots assisted and NIR-II Emissive In Vivo Two-photon microscopy

2021 ◽  
Author(s):  
Huwei Ni ◽  
Yalun Wang ◽  
Tao Tang ◽  
Wenbin Yu ◽  
Dongyu Li ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Two Photon Microscopy ◽  
Two Photon

Video-rate two-photon microscopy of cortical hemodynamics in-vivo

2006 ◽  
Author(s):  
Matthew Bouchard ◽  
Svetlana Ruvinskya ◽  
David A. Boas ◽  
Elizabeth M. C. Hillman
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Impact of Body Site, Age, and Gender on the Collagen/Elastin Index by Noninvasive in vivo Vertical Two-Photon Microscopy

2017 ◽  
Vol 30 (5) ◽  
pp. 260-267 ◽  
Author(s):  
Carolin Czekalla ◽  
Karl-Heinz Schönborn ◽  
Nadine Döge ◽  
Sora Jung ◽  
Maxim E. Darvin ◽  
...  
Keyword(s):  
Body Site ◽  
Age And Gender ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
And Gender
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