scholarly journals Three-dimensional Optical-resolution Photoacoustic Microscopy

10.3791/2729 ◽  
2011 ◽  
Author(s):  
Song Hu ◽  
Konstantin Maslov ◽  
Lihong V. Wang
Keyword(s):  
Three Dimensional ◽  
Optical Resolution ◽  
Photoacoustic Microscopy

scholarly journals Optical Resolution Photoacoustic Microscopy of Ovary and Fallopian Tube

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Bin Rao ◽  
Xiandong Leng ◽  
Yifeng Zeng ◽  
Yixiao Lin ◽  
Ruimin Chen ◽  
...  
Keyword(s):  
Fallopian Tube ◽  
Ovarian Tissue ◽  
Three Dimensional ◽  
Optical Resolution ◽  
Optical Biopsy ◽  
Photoacoustic Microscopy ◽  
Uniform Size ◽  
Imaging Tool ◽  
Vascular Segment ◽  
Vessel Volume

Abstract Ovarian cancer is the leading cause of death among gynecological cancers, but is poorly amenable to preoperative diagnosis. In this study, we investigate the feasibility of “optical biopsy,” using high-optical-resolution photoacoustic microscopy (OR-PAM) to quantify the microvasculature of ovarian and fallopian tube tissue. The technique is demonstrated using excised human ovary and fallopian tube specimens imaged immediately after surgery. Quantitative parameters are derived using Amira software. The parameters include three-dimensional vascular segment count, total volume and length, which are associated with tumor angiogenesis. Qualitative results of OR-PAM demonstrate that malignant ovarian tissue has larger and more tortuous blood vessels as well as smaller vessels of different sizes, while benign and normal ovarian tissue has smaller vessels of uniform size. Quantitative analysis shows that malignant ovaries have greater tumor vessel volume, length and number of segments, as compared with benign and normal ovaries. The vascular pattern of benign fallopian tube is different than that of benign ovarian tissue. Our initial results demonstrate the potential of OR-PAM as an imaging tool for fast assessment of ovarian tissue and fallopian tube and could avoid unnecessary surgery if the risk of the examined ovary is extremely low.

scholarly journals Method for the biomechanical analysis of aqueous veins and perilimbal sclera by three-dimensional photoacoustic imaging and strain field calculation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Linyu Ni ◽  
John Riesterer ◽  
Huaizhou Wang ◽  
Layla Berry ◽  
Kara Blackburn ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Photoacoustic Imaging ◽  
Three Dimensional ◽  
Optical Resolution ◽  
Element Model ◽  
Biological Tissues ◽  
Biomechanical Analysis ◽  
Field Calculation ◽  
Photoacoustic Microscopy ◽  
Strain Calculation

AbstractA method motivated by the eye’s aqueous veins is described for the imaging and strain calculation within soft biological tissues. A challenge to the investigation of the biomechanics of the aqueous vein—perilimbal sclera tissue complex is resolution of tissue deformations as a function of intraocular pressure and the subsequent calculation of strain (a normalized measure of deformation). The method involves perfusion of the eye with a contrast agent during conduction of non-invasive, optical resolution photoacoustic microscopy. This imaging technique permits three-dimensional displacement measurements of tracked points on the inner walls of the veins which are used in a finite element model to determine the corresponding strains. The methods are validated against two standard strain measurement methods. Representative porcine globe perfusion experiments are presented that demonstrate the power of the method to determine complex strain fields in the veins dependent on intraocular pressure as well as vein anatomy. In these cases, veins are observed to move radially outward during increases in intraocular pressure and to possess significant spatial strain variation, possibly influenced by their branching patterns. To the authors’ knowledge, these are the only such quantitative, data driven, calculations of the aqueous vein strains available in the open literature.

scholarly journals Three-dimensional label-free imaging of mammalian yolk sac vascular remodeling with optical resolution photoacoustic microscopy

2020 ◽  
Vol 17 ◽  
pp. 100152 ◽  
Author(s):  
Doudou Huang ◽  
Yali Huang ◽  
Qi Qiu ◽  
Kai Wang ◽  
Zhihong Li ◽  
...  
Keyword(s):  
Vascular Remodeling ◽  
Three Dimensional ◽  
Optical Resolution ◽  
Yolk Sac ◽  
Photoacoustic Microscopy ◽  
Label Free
Sign in / Sign up

Export Citation Format

Share Document