scholarly journals The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

10.3791/2259 ◽  
2010 ◽  
Author(s):  
Benjamin J. C. Quah ◽  
Christopher R. Parish
Keyword(s):  
Lymphocyte Proliferation ◽  
Succinimidyl Ester ◽  
Diacetate Succinimidyl

The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo

1999 ◽  
Vol 77 (6) ◽  
pp. 539-543 ◽  
Author(s):  
Justine Mintern ◽  
Ming Li ◽  
Gayle M Davey ◽  
Effrossini Blanas ◽  
Christian Kurts ◽  
...  
Keyword(s):  
Antigen Presentation ◽  
Cell Type ◽  
Succinimidyl Ester ◽  
Diacetate Succinimidyl

Carboxyfluorescein diacetate succinimidyl ester labeling method to study the interaction between Leptospira and macrophages

2014 ◽  
Vol 107 ◽  
pp. 205-213 ◽  
Author(s):  
Boyu Liu ◽  
Yanchun Wang ◽  
Xiaokui Guo ◽  
Weinan Zhu ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Succinimidyl Ester ◽  
Labeling Method ◽  
Diacetate Succinimidyl

scholarly journals Cd4+ T Cell Division in Irradiated Mice Requires Peptides Distinct from Those Responsible for Thymic Selection

1999 ◽  
Vol 190 (3) ◽  
pp. 367-374 ◽  
Author(s):  
Jeremy Bender ◽  
Tom Mitchell ◽  
John Kappler ◽  
Philippa Marrack
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd4 T Cells ◽  
Class Ii ◽  
Cd4 T Cell ◽  
Thymic Selection ◽  
Cell Numbers ◽  
Succinimidyl Ester ◽  
Diacetate Succinimidyl ◽  
Sublethal Irradiation

We investigated the mechanism by which α/β T cells expand upon transfer to T cell–deficient host mice by injecting carboxyfluorescein diacetate succinimidyl ester–labeled T cells into mice depleted of T cells by sublethal irradiation. We found that CD4+ T cells divided when transferred to irradiated hosts and that the division of more than half of these cells required class II expression. However, division of transferred CD4+ T cells did not occur in irradiated hosts that expressed class II molecules occupied solely by the peptide responsible for thymic selection, indicating that peptides distinct from those involved in thymic selection cause the division of CD4+ T cells in irradiated mice. These data establish that class II–bound peptides control the expansion of CD4+ T cells transferred to T cell–deficient hosts and suggest that the same peptides contribute to the maintenance of T cell numbers in normal mice.

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