Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

2007 ◽  
Vol 2 (9) ◽  
pp. 2049-2056 ◽  
Author(s):  
Ben J C Quah ◽  
Hilary S Warren ◽  
Christopher R Parish
Keyword(s):  
Lymphocyte Proliferation ◽  
Fluorescent Dye ◽  
Succinimidyl Ester ◽  
Proliferation In Vitro ◽  
Diacetate Succinimidyl

Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo

2002 ◽  
Vol 30 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Amelia Bartholomew ◽  
Cord Sturgeon ◽  
Mandy Siatskas ◽  
Karen Ferrer ◽  
Kevin McIntosh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graft Survival ◽  
Skin Graft ◽  
Lymphocyte Proliferation ◽  
Proliferation In Vitro

Suppressive effect of AKR mouse blast cells on antibody formation in vivo and lymphocyte proliferation in vitro

1988 ◽  
Vol 105 (2) ◽  
pp. 212-215 ◽  
Author(s):  
N. V. Kashlakova ◽  
I. A. Lisukov ◽  
I. G. Tsyrlova ◽  
N. V. Vasil'ev ◽  
V. A. Kozlov
Keyword(s):  
Lymphocyte Proliferation ◽  
Antibody Formation ◽  
Suppressive Effect ◽  
Blast Cells ◽  
Proliferation In Vitro

The cyclo-oxygenase inhibitor, Piroxicam, enhances cytokine-induced lymphocyte proliferation in vitro and in vivo

1990 ◽  
Vol 68 (4) ◽  
pp. 225-230 ◽  
Author(s):  
D. R. Haynes ◽  
P. F. Wright ◽  
M. W. Whitehouse ◽  
B. Vernon-Roberts
Keyword(s):  
Lymphocyte Proliferation ◽  
Proliferation In Vitro

Effects of Astragalus polysaccharide liposome on lymphocyte proliferation in vitro and adjuvanticity in vivo

2012 ◽  
Vol 88 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Yunpeng Fan ◽  
Yuanliang Hu ◽  
Deyun Wang ◽  
Jiaguo Liu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Astragalus Polysaccharide ◽  
Proliferation In Vitro

scholarly journals Micro/nano-textured hierarchical titanium topography promotes exosome biogenesis and secretion to improve osseointegration

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Zhengchuan Zhang ◽  
Ruogu Xu ◽  
Yang Yang ◽  
Chaoan Liang ◽  
Xiaolin Yu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Titanium Surface ◽  
Titanium Implants ◽  
Extracellular Secretion ◽  
Exosome Biogenesis ◽  
Marrow Mesenchymal Stem Cells ◽  
Proliferation In Vitro ◽  
Extracellular Environment

Abstract Background Micro/nano-textured hierarchical titanium topography is more bioactive and biomimetic than smooth, micro-textured or nano-textured titanium topographies. Bone marrow mesenchymal stem cells (BMSCs) and exosomes derived from BMSCs play important roles in the osseointegration of titanium implants, but the effects and mechanisms of titanium topography on BMSCs-derived exosome secretion are still unclear. This study determined whether the secretion behavior of exosomes derived from BMSCs is differently affected by different titanium topographies both in vitro and in vivo. Results We found that both micro/nanonet-textured hierarchical titanium topography and micro/nanotube-textured hierarchical titanium topography showed favorable roughness and hydrophilicity. These two micro/nano-textured hierarchical titanium topographies enhanced the spreading areas of BMSCs on the titanium surface with stronger promotion of BMSCs proliferation in vitro. Compared to micro-textured titanium topography, micro/nano-textured hierarchical titanium topography significantly enhanced osseointegration in vivo and promoted BMSCs to synthesize and transport exosomes and then release these exosomes into the extracellular environment both in vitro and in vivo. Moreover, micro/nanonet-textured hierarchical titanium topography promoted exosome secretion by upregulating RAB27B and SMPD3 gene expression and micro/nanotube-textured hierarchical titanium topography promoted exosome secretion due to the strongest enhancement in cell proliferation. Conclusions These findings provide evidence that micro/nano-textured hierarchical titanium topography promotes exosome biogenesis and extracellular secretion for enhanced osseointegration. Our findings also highlight that the optimized titanium topography can increase exosome secretion from BMSCs, which may promote osseointegration of titanium implants.

Effects of retinoic acid steroidal analogs on human leukemic HL60 cell proliferation in vitro and on angiogenesis in vivo

2005 ◽  
Vol 16 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Evaggelia S. Arsenou ◽  
Evangelia P. Papadimitriou ◽  
Eleni Kliafa ◽  
Maria Hountala ◽  
Sotiris S. Nikolaropoulos
Keyword(s):  
Cell Proliferation ◽  
Retinoic Acid ◽  
Hl60 Cell ◽  
Proliferation In Vitro
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