scholarly journals Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

10.3791/2244 ◽  
2010 ◽  
Author(s):  
Dmitry A. Molotkov ◽  
Alexey Y. Yukin ◽  
Ramil A. Afzalov ◽  
Leonard S. Khiroug
Keyword(s):  
Gene Delivery ◽  
Rat Brain ◽  
Plasmid Injection

Herpesvirus-mediated gene delivery into the rat brain: specificity and efficiency of the neuron-specific enolase promoter

1993 ◽  
Vol 13 (5) ◽  
pp. 503-515 ◽  
Author(s):  
Julie K. Andersen ◽  
David M. Frim ◽  
Ole Isacson ◽  
Xandra O. Breakefield
Keyword(s):  
Gene Delivery ◽  
Rat Brain ◽  
Neuron Specific Enolase

scholarly journals Serotype-based evaluation of an optogenetic construct in rat cortical astrocytes

2021 ◽  
Author(s):  
Lakshmini Balachandar ◽  
Diana Borrego ◽  
Jorge Riera
Keyword(s):  
Gene Delivery ◽  
Rat Brain ◽  
Neuronal Cell ◽  
Cell Types ◽  
Transgenic Animal ◽  
Transduction Efficiency ◽  
Aav Vector ◽  
Viral Spread ◽  
Cortical Astrocytes

Optogenetics is a modern technique which has been recently expanded to non-neuronal cell types, e.g., astrocytes, and involves targeted gene delivery of light-sensitive ion channels like Channelrhodopsin-2 (ChR2). Optogenetic regulation of astrocytic activity can be used for therapeutic intervention of several neurological disorders. Astrocytic gene delivery, viz adeno-associated viral (AAV) vectors, have proven to be robust, time-, and cost-efficient contrary to the generation of transgenic animal models. When transducing astrocytes with an AAV vector, it is imperative to perform a serotype evaluation of the AAV vector due to variability in serotype transduction efficiency depending on species, target region and construct length. Rats have been a very successful animal model for studying a variety of brain disorders, from which ChR2-based intervention of astrocytes will benefit. However, the most efficient AAV capsid serotype targeting astrocytes for ChR2 expression in the in vivo rat brain cortex has not been characterized. To address this, we have evaluated AAV serotypes 1, 5, and 8 of the vector AAV-GFAP-hChR2(H134)-mCherry targeting astrocytes in the rat brain neocortex. Results show that serotype 8 exhibits promising transduction patterns, as it has demonstrated the highest tangential and radial viral spread in the rat brain. Our research will facilitate translational research for future applications of optogenetics involving the transduction of rat brain cortical astrocytes.

Gene delivery to glioma cells in rat brain by grafting of a retrovirus packaging cell line

1990 ◽  
Vol 27 (3) ◽  
pp. 427-439 ◽  
Author(s):  
M. P. Short ◽  
B. C. Choi ◽  
J. K. Lee ◽  
A. Malick ◽  
X. O. Breakefield ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Rat Brain ◽  
Cell Line ◽  
Glioma Cells ◽  
Packaging Cell Line ◽  
Packaging Cell

cDNA cloning and expression of a gamma-aminobutyric acidA receptor epsilon-subunit in rat brain

2000 ◽  
Vol 12 (12) ◽  
pp. 4318-4330 ◽  
Author(s):  
Nathalie Moragues ◽  
Philippe Ciofi ◽  
Pierrette Lafon ◽  
Marie-Francoise Odessa ◽  
Gerard Tramu ◽  
...  
Keyword(s):  
Rat Brain ◽  
Cdna Cloning ◽  
Cloning And Expression ◽  
Epsilon Subunit
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