Herpesvirus-mediated gene delivery into the rat brain: specificity and efficiency of the neuron-specific enolase promoter

1993 ◽  
Vol 13 (5) ◽  
pp. 503-515 ◽  
Author(s):  
Julie K. Andersen ◽  
David M. Frim ◽  
Ole Isacson ◽  
Xandra O. Breakefield
Keyword(s):  
Gene Delivery ◽  
Rat Brain ◽  
Neuron Specific Enolase

scholarly journals Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

10.3791/2244 ◽  
2010 ◽  
Author(s):  
Dmitry A. Molotkov ◽  
Alexey Y. Yukin ◽  
Ramil A. Afzalov ◽  
Leonard S. Khiroug
Keyword(s):  
Gene Delivery ◽  
Rat Brain ◽  
Plasmid Injection

N-acetyl-l-cysteine attenuates oxidative damage and neurodegeneration in rat brain during aging

2018 ◽  
Vol 96 (12) ◽  
pp. 1189-1196 ◽  
Author(s):  
Geetika Garg ◽  
Sandeep Singh ◽  
Abhishek Kumar Singh ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Rat Brain ◽  
Neuroprotective Effect ◽  
Neuron Specific Enolase ◽  
Sirtuin 1 ◽  
Marker Genes ◽  
Aging Brain ◽  
Age Related ◽  
Nonenzymatic Antioxidants ◽  
Old Rats ◽  
The Brain

N-acetyl-l-cysteine (NAC) is a precursor of cysteine, which is known to increase the level of glutathione (GSH) in the brain. Several neurodegenerative changes linked to oxidative stress take place in the aging brain. This study aimed to assess the neuroprotective effect of NAC supplementation on age-dependent neurodegeneration in the rat brain. Young (4 months) and old (24 months) Wistar rats (n = 6 rats/group) were supplemented with NAC (100 mg/kg b.w. orally) for 14 days. Enzymatic and nonenzymatic antioxidants such as superoxide dismutase and catalase, and GSH and total thiol respectively, prooxidants such as protein carbonyl, advanced oxidation protein products, reactive oxygen species, and malondialdehyde were assessed in the brain homogenates. Furthermore, nitric oxide level, acetylcholinesterase activity, and Na+/K+–ATPase activity were measured and gene expression studies were also performed. The results indicated that NAC augmented the level of enzymatic and nonenzymatic antioxidants with a significant reduction in prooxidant levels in old rats. NAC supplementation also downregulated the expression of inflammatory markers (TNF-α, IL-1β, IL-6) and upregulated the expression of marker genes associated with aging (sirtuin-1) and neurodegeneration (neuron-specific enolase, neuroglobin, synapsin-I, myelin basic protein 2) in old rats. The present findings support a neuroprotective role of NAC which has therapeutic implication in controlling age-related neurological disorders.

scholarly journals Characterization of translation products of the polyadenylated RNA of free and membrane-bound polyribosomes of rat forebrain

1984 ◽  
Vol 219 (3) ◽  
pp. 751-761 ◽  
Author(s):  
C Hall ◽  
L Mahadevan ◽  
S Whatley ◽  
G Biswas ◽  
L Lim
Keyword(s):  
Rat Brain ◽  
Plasma Membranes ◽  
Neuron Specific Enolase ◽  
Two Dimensional ◽  
Synaptic Plasma Membranes ◽  
Two Dimensional Gel Electrophoresis ◽  
Specific Product ◽  
Polyadenylated Rna ◽  
Microsomal Membranes ◽  
Membrane Bound

Poly(A)+ RNA (polyadenylated RNA) isolated from membrane-bound and free polyribosomes was translated in reticulocyte lysates, and the products were analysed by two-dimensional gel electrophoresis. Several translation products were specific to membrane-bound polyribosomal mRNA, including polypeptides of 47kDa, 35kDa and 21 kDa, whereas others (e.g. of 37 kDa, 17 kDa and 14 kDa) were specific to free polyribosomal mRNA. Although many products were common to both mRNA species, cross-contamination could be ruled out on the basis of the presence of these and other specific products. The common products included a 68 kDa microtubule-associated protein, tubulin, actin, the brain form of creatine kinase, neuron-specific enolase and protein 14-3-3 and calmodulin, all of which were identified on the basis of two-dimensional gel and peptide analyses. The 35 kDa protein product of membrane-specific mRNA was co-translationally processed in vitro by microsomal membranes, resulting in its cleavage to 33 kDa (and partial glycosylation). The 33 kDa processed protein (but not the 35 kDa precursor) was integrated into both dog pancreas and rat brain microsomal membranes. The occurrence of the enzymes and calmodulin as products of membrane-bound polyribosomal mRNA is discussed in the light of their presence on rat brain synaptic plasma membranes [Lim, Hall, Leung, Mahadevan & Whatley (1983) J. Neurochem. 41, 1177-1182] and their existence in a specific component of axonal flow. It is suggested that some of these translation products of the rough endoplasmic reticulum may represent proteins destined for the plasma membrane. However, the identity and location of the 35 kDa membrane-specific product (or its processed form) still remain unestablished.

Immunocytochemistry of Neuron Specific Enolase (NSE) in the Rat Brain After Single and Repeated Epileptic Seizures

2008 ◽  
Vol 118 (7) ◽  
pp. 981-993 ◽  
Author(s):  
Melda Yardimoğlu ◽  
Gül İlbay ◽  
Cannur Dalçik ◽  
Hakki Dalçik ◽  
Deni˙z Sahi˙n ◽  
...  
Keyword(s):  
Rat Brain ◽  
Neuron Specific Enolase ◽  
Epileptic Seizures

Expression of Neuron-Specific Enolase in Adult Rat Brain Following Status Epilepticus

1999 ◽  
Vol 159 (1) ◽  
pp. 329-331 ◽  
Author(s):  
Steven S. Schreiber ◽  
Ning Sun ◽  
Georges Tocco ◽  
Michel Baudry ◽  
Christopher M. DeGiorgio
Keyword(s):  
Status Epilepticus ◽  
Rat Brain ◽  
Neuron Specific Enolase ◽  
Adult Rat ◽  
Adult Rat Brain
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