scholarly journals Molecular-genetic identification of chameleon goby Tridentiger trigonocephalus (Gill, 1859) in the Black Sea

2020 ◽  
Vol 32 ◽  
pp. 46-52
Author(s):  
Yuriy V. Slynko ◽  
Elena E. Slynko ◽  
Ernes R. Abliazov ◽  
Elena G. Skvortsova ◽  
Oksana V. Filinskaya
Keyword(s):  
Black Sea ◽  
Molecular Genetic ◽  
Far East ◽  
Genetic Identification ◽  
The Black Sea ◽  
Lepomis Gibbosus ◽  
Rrna Gene ◽  
Similar Data ◽  
Natural Range ◽  
Molecular Genetic Identification

The article focuses on the molecular-genetic identification of the chameleon goby, which has recently become established in the Black Sea basin. The natural range of the chameleon goby are the seas of the Far-East. Genetic variation was studied for the16S rRNA mtDNA gene. The obtained genetic data were compared with similar data of chameleon goby from the natural range and other species from the Tridentiger genus. We determined that, according to the 16S rRNA gene, the identification of the goby from the mouth of the Chernaya River as a species of Tridentiger trigonocephalus was confirmed, and the parameters of the variability indicate an extremely low genetic variability for this gene. According to the authors, the adaptive success of the chameleon goby in the conditions of the Black Sea basin is similar to such of invasive species, namely pumpkinseed (Lepomis gibbosus) and stone moroko (Pseudorasbora parva).

scholarly journals Molecular-genetic identification of microorganism strains isolated from activated sludge based on an analysis of nucleotide sequences of the 16s rRNA gene

2021 ◽  
Vol 11 (1) ◽  
pp. 116-124
Author(s):  
E. A. Ivanchikov ◽  
A. T. Bubeev ◽  
V. Zh. Tsyrenov ◽  
A. V. Arbatsksaya
Keyword(s):  
16S Rrna ◽  
Activated Sludge ◽  
Lipolytic Activity ◽  
Molecular Genetic ◽  
Paenibacillus Polymyxa ◽  
Biochemical Properties ◽  
Genetic Identification ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Molecular Genetic Identification

A molecular-genetic identification of four bacterial strains isolated from activated sludge of urban wastewater treatment plants (Ulan-Ude) and the industrial enterprise OJSC “Selenginsky Pulp and Paper Mill” (Selenginsk) was carried out. Bacterial strains were identified by a capillary sequencer ABI 3130XL Genetic Analyzer (Applied Biosystems) using 16S primers 27F and 1492R at the Genomics Collective Use Center of the Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk. The results were obtained using the method of determination of the direct nucleotide sequence of a 16S rRNA fragment followed by comparison of the nucleotide identity with the sequences deposited in the international database GenBank. Bacterial strains isolated from activated sludge were identified according to the GenBank database: strain B 1.1 corresponds to Paenibacillus dendritiformis strain P411 (similarity 99.93%), strains B 1.2 and B 1.3 correspond to Bacillus licheniformis strain PB399 (similarity 86 and 100%, respectively), strain P 1.1 corresponds to the Paenibacillus polymyxa strain ISSDS-85 (similarity 99.86%). The biochemical properties of the identified strains were determined: amylolytic, proteolytic and lipolytic activity; the ability to ferment carbohydrates in Hiss’ nutrient medium; the ability to form ammonia, urea and nitrate reduction. The bacterial strains isolated from activated sludge may be promising for the destruction of wastewater pollutants. On their basis, it is planned to create a consortium of microorganisms for the destruction of protein and fatty pollutants in wastewater.

Molecular genetic identification of isolates of the hepatitis A virus (HAV) from monkeys at Adler Primate Center

2018 ◽  
Vol 47 (2) ◽  
pp. 87-92 ◽  
Author(s):  
Dmitriy I. Dogadov ◽  
Lydia I. Korzaya ◽  
Anastasia A. Karlsen ◽  
Karen K. Kyuregyan
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Molecular Genetic ◽  
Genetic Identification ◽  
Molecular Genetic Identification

scholarly journals Selecting of Polymorphic Loci of Genome for Identification of Populations of Pinus sylvestris L. on East-Europe Plain

2019 ◽  
Vol 5 (5) ◽  
pp. 25-30
Author(s):  
Ya. Prishnivskaya ◽  
E. Nassonova ◽  
Yu. Vasileva ◽  
S. Boronnikova
Keyword(s):  
Pinus Sylvestris ◽  
Molecular Genetic ◽  
Genetic Identification ◽  
East European Plain ◽  
Data Bases ◽  
East Europe ◽  
Polymorphic Loci ◽  
East European ◽  
Molecular Genetic Identification

10 pairs of primers from 8 related Pinus sylvestris L. populations collected on East-European plain to 10 genes and 4 primer’s pairs to 4 loci of uncoding clDNA regions. 2 loci of uncoding clDNA regions (psbA-trnH, trnL-trnF) were selected from tested 14 primer’s pairs. These two loci are most polymorphic and has homologous consistencies in data bases. Therefore, these loci is recommended for molecular–genetic identification of related Pinus sylvestris L. populations on East–European plain.

scholarly journals Study of European and Czech populations of potato cyst nematodes (Globodera rostochiensis and G. pallida) by RAPD method

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 70-74 ◽  
Author(s):  
P. Sedlák ◽  
M. Melounová ◽  
S. Skupinová ◽  
P. Vejl ◽  
J. Domkářová
Keyword(s):  
Animal Species ◽  
Molecular Genetic ◽  
Globodera Rostochiensis ◽  
Globodera Pallida ◽  
Genetic Identification ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Genetic Dissimilarity ◽  
Solanaceous Plants ◽  
Molecular Genetic Identification

Potato cyst nematodes (PCN) are the big problem in worldwide planting of potatoes and another Solanaceous plants. Identification of individual pathotypes according to international scheme is very demanding but a very important part of the phytosanitary process to control these pests. Molecular genetic identification of different plant and animal species or individuals is a very interesting way at the present time and let’s hope that it will be important in future. This report presents results of the RAPD study of nine different real PCN populations. There were five Globodera rostochiensis populations and four G. pallida populations. Pathotypes Ro2, Ro2/3, Ro4, Ro5, Pa2 and Pa3 were from European populations; population Ro1 and X were of Czech provenance. Genetics variable of these populations was described by a set of six decameric primers (OPA 07, OPG 03, OPG 05, OPG 08, OPG 10 and OPG 13). Genetic dissimilarity was by Gel Manager for Windows evaluated. Detectable differences behind all populations were found and the dendrogram was compiled. The unknown population X was sorted into group of Globodera pallida species subgroup of Pa2 consequently.

Molecular genetic identification of southern hemisphere beaked whales (Cetacea: Ziphiidae)

1998 ◽  
Vol 7 (6) ◽  
pp. 687-694 ◽  
Author(s):  
M. L. DALEBOUT ◽  
A. VAN HELDEN ◽  
K. VAN WAEREBEEK ◽  
C. S. BAKER
Keyword(s):  
Southern Hemisphere ◽  
Molecular Genetic ◽  
Genetic Identification ◽  
Beaked Whales ◽  
Molecular Genetic Identification

scholarly journals Diversity and Distribution of Planctomycetes and Related Bacteria in the Suboxic Zone of the Black Sea

2006 ◽  
Vol 72 (4) ◽  
pp. 3079-3083 ◽  
Author(s):  
John Kirkpatrick ◽  
Brian Oakley ◽  
Clara Fuchsman ◽  
Sujatha Srinivasan ◽  
James T. Staley ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Black Sea ◽  
Gene Sequence ◽  
The Black Sea ◽  
Anammox Bacteria ◽  
Rrna Gene ◽  
Sequence Information ◽  
Rrna Gene Sequence ◽  
Suboxic Zone

ABSTRACT Samples from six depths of the Black Sea's suboxic zone were analyzed for 16S rRNA gene sequence information. A gradient in phylotype diversity was found. The distributions of known anaerobic ammonium oxidation (anammox) bacteria, many unknown Planctomycetes, and other phylotypes were examined in relation to the local nutrient and redox conditions.

Sign in / Sign up

Export Citation Format

Share Document