scholarly journals MORPHOLOGICAL AND MORPHOMETRICAL FEATURES IN DUNALIELLA SALINA (CHLAMYDOMONADALES, DUNALIELLACEAE) DURING THE TWO-PHASE CULTIVATION MODE

2019 ◽  
Vol 22 ◽  
pp. 157-165
Author(s):  
Andrei B. Borovkov ◽  
Irina N. Gudvilovich ◽  
Olga A. Memetshaeva ◽  
Anna L. Avsiyan ◽  
Alexander S. Lelekov ◽  
...  

The paper presents studies of morphological and morphometrical characteristics of green halophilic carotenogenic microalga Dunaliella salina (Dunal) Teodoresco, 1905, from the south-west region of Crimean Peninsula. D. salina was cultivated in two-phase mode under conditions of natural illumination at the premises of A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (IBSS), Sevastopol, Russia. The maximum D. salina cell density was 1.69·106 cell/ml in the “green” phase and 0.84·106 cell/ml in the “red” growth phase. The maximum productivity by cell number reached 0.15 ∙ 106 cell/(ml·day)in the “green” phase while it was lower by 73% in the “red” phase (0.04∙106 cell/(ml·day). Along with the maximum productivity, linear growth stage in the first phase was characterized by a maximum fraction of small (up to 500 μm3 in volume) cells (about 15-29%) and a decrease in cell volume by 40-45% as compared with initial value. The mean of D. salina cell volume in the “red” phase was 30% higher than in the “green” phase. At the same time, the large cell fraction in the “red” phase was consistently high (15-35%). The patterns of change in morphological and morphometrical cell parameters were in accordance to stage and conditions of growth. Thus, cell elongation was noted in the stage of linear growth, while under unfavorable conditions at growth-declining stage cells became more round-shaped, with orange and tile-red coloration and granulation of cell content. It was shown that morphological and morphometric cell parameters can serve as additional criteria for assessment of physiological condition in D. salina culture. The experiment demonstrated the prospects for two-stage D. salina cultivation in Crimea.

2021 ◽  
Vol 48 (9) ◽  
Author(s):  
G. Diego Gatta ◽  
Francesco Pagliaro ◽  
Paolo Lotti ◽  
Alessandro Guastoni ◽  
Laura Cañadillas-Delgado ◽  
...  

AbstractThe thermal behaviour of a natural allanite-(Ce) has been investigated up to 1073 K (at room pressure) by means of in situ synchrotron powder X-ray diffraction and single-crystal neutron diffraction. Allanite preserves its crystallinity up to 1073 K. However, up to 700 K, the thermal behaviour along the three principal crystallographic axes, of the monoclinic β angle and of the unit-cell volume follow monotonically increasing trends, which are almost linear. At T > 700–800 K, a drastic change takes place: an inversion of the trend is observed along the a and b axes (more pronounced along b) and for the monoclinic β angle; in contrast, an anomalous increase of the expansion is observed along the c axis, which controls the positive trend experienced by the unit-cell volume at T > 700–800 K. Data collected back to room T, after the HT experiments, show unit-cell parameters significantly different with respect to those previously measured at 293 K: allanite responds with an ideal elastic behaviour up to 700 K, and at T > 700–800 K its behaviour deviates from the elasticity field. The thermo-elastic behaviour up to 700 K was modelled with a modified Holland–Powell EoS; for the unit-cell volume, we obtained the following parameters: VT0 = 467.33(6) Å3 and αT0(V) = 2.8(3) × 10–5 K−1. The thermal anisotropy, derived on the basis of the axial expansion along the three main crystallographic directions, is the following: αT0(a):αT0(b):αT0(c) = 1.08:1:1.36. The T-induced mechanisms, at the atomic scale, are described on the basis of the neutron structure refinements at different temperatures. Evidence of dehydroxylation effect at T ≥ 848 K are reported. A comparison between the thermal behaviour of allanite, epidote and clinozoisite is carried out.


2021 ◽  
pp. 1-3
Author(s):  
J. Maixner ◽  
J. Ryšavý

X-ray powder diffraction data, unit-cell parameters, and space group for tetrazene nitrate monohydrate, C2H9N11O4, are reported [a = 5.205(1) Å, b = 13.932(3) Å, c = 14.196(4) Å, β = 97.826(3)°, unit-cell volume V = 1019.8(4) Å3, Z = 4, and space group P21/c]. All measured lines were indexed and are consistent with the P21/c space group. No detectable impurities were observed.


2013 ◽  
Vol 28 (4) ◽  
pp. 296-298
Author(s):  
R. Pažout ◽  
J. Maixner ◽  
A.S. Jones ◽  
J. Merna

X-ray powder diffraction data, unit-cell parameters, and space group for a new bis(β-diiminato) Cu(II) complex, C44H54CuN4O4, are reported [a = 8.683(3) Å, b = 11.216(3) Å, c = 11.753(4) Å, α = 66.27(3), β = 84.61(3), γ = 78.85(3), unit-cell volume V = 1027.77 Å3, Z = 1, and space group P-1]. All measured lines were indexed and are consistent with the P-1 space group. No detectable impurity was observed.


2013 ◽  
Vol 28 (3) ◽  
pp. 231-233 ◽  
Author(s):  
Li Li Zhang ◽  
Qing Qing Pan ◽  
Dan Xiao ◽  
Xiao Qing Wu ◽  
Qing Wang ◽  
...  

X-ray powder diffraction data, unit-cell parameters, and space group for deoxyschisandrin, C24H32O6, are reported [a = 13.083(3) Å, b = 19.563(9) Å, c = 8.805(6) Å, β = 90.472(0)°, unit-cell volume V = 2253.82 Å3, Z = 4, and space group P21]. All measured lines were indexed and are consistent with the P21 space group. No detectable impurity was observed.


2018 ◽  
Vol 33 (4) ◽  
pp. 327-329
Author(s):  
Wan Wang ◽  
Zili Suo ◽  
Lidong Liao ◽  
Hui Li

X-ray powder diffraction (XRD) data, unit-cell parameters and space group for 7-ethyl-14-nitro-camptothecin, C22H19N3O6, are reported [a = 10.987(5) Å, b = 10.941 (9) Å, c = 8.438 (2) Å, α = 71.321(6)°, β = 96.145(0)°, γ = 95.139(3)°, unit-cell volume V = 953.87 Å3, Z = 2, ρcal = 1.467 g cm−3, and space group P-1]. All measured lines were indexed and are consistent with the P-1 space group. No detectable impurities were observed.


2019 ◽  
Vol 287 ◽  
pp. 121398 ◽  
Author(s):  
Yixing Sui ◽  
Maarten Muys ◽  
Dedmer B. Van de Waal ◽  
Sarah D'Adamo ◽  
Pieter Vermeir ◽  
...  

1962 ◽  
Vol 40 (1) ◽  
pp. 437-442 ◽  
Author(s):  
W. Zingg ◽  
A. Angel ◽  
M. D. Steinberg

The changes in number and volume of fat cells accompanying changes in the size of the perirenal fat depots of rats induced by dietary and other means have been investigated by direct histological examination and by estimation of the total desoxyribonucleic acid (DNA) and fat content. With both methods, an increase in cell volume and in cell number was found to accompany an increase in depot volume.


2020 ◽  
Vol 83 (3) ◽  
pp. 279-286
Author(s):  
Zhen Zhang ◽  
Jing Guo ◽  
Xiaobin Li ◽  
Chuanling Li ◽  
Xiaolin Ma ◽  
...  

Background: Mild encephalitis/encephalopathy with a reversible isolated splenial lesion (MERS) is a rare and complex clinico-radiological condition that aggregates various diseases. From a geographical point of view, most cases have been reported in Asia, especially in Japan. However, a very limited number of studies have properly evaluated Chinese patients diagnosed with MERS. Methods: Putative encephalitis/encephalopathy patients affected by a reversible isolated SCC lesion, as detected by magnetic resonance imaging (MRI), were retrospectively evaluated. Specifically, we reviewed extensive patient data, including prodromal and neurologic symptoms, neurologic examinations, MRI and electroencephalography (EEG) findings, laboratory results, treatments, and prognosis. Results: Six clinically mild encephalitis/encephalopathy patients, affected by a reversible isolated SCC lesion, were identified in a cohort of 22 patients. Six patients presented fever symptoms before the onset of neurological manifestations. Initial MRI data exhibited isolated SCC lesions with isointense or slightly hypointense on T1WI, hyperintense on T2WI, Flair, and DWI, as well as decreased ADC values, without contrast enhancement. EEG data indicated abnormalities (3/4), as well as elevated serum inflammatory markers (5/6) and hyponatremia (4/6). Cell content and protein yields were normal or slightly increased in the CSF of 6 patients, excluding one episode of significant increase in cell number. Patients were treated with corticosteroids, antivirals, and antibiotics, resulting in their full clinical recovery. SCC lesions mostly disappeared in all patients. Conclusion: In our study, the clinical features identified in all affected patients were mostly identical, which consisted of relatively mild CNS manifestations with a promising prognostic status.


2018 ◽  
Vol 13 (9-10) ◽  
pp. 539-545 ◽  
Author(s):  
D. S. Chumakov ◽  
A. O. Sokolov ◽  
V. A. Bogatyrev ◽  
O. I. Sokolov ◽  
N. Yu. Selivanov ◽  
...  

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