scholarly journals Objective observations in process of diagnosis by acupuncture and moxibustion. 5. Structural analysis of Fukushou by applying image measurement methods.

Author(s):  
Syohji KOBAYASHI ◽  
Kazu MORI
2001 ◽  
Author(s):  
Hans van der Laan ◽  
Marcel Dierichs ◽  
Henk van Greevenbroek ◽  
Elaine McCoo ◽  
Fred Stoffels ◽  
...  

Author(s):  
T. Luhmann

This paper discusses a feature of projective geometry which causes eccentricity in the image measurement of circular and spherical targets. While it is commonly known that flat circular targets can have a significant displacement of the elliptical image centre with respect to the true imaged circle centre, it can also be shown that the a similar effect exists for spherical targets. Both types of targets are imaged with an elliptical contour. As a result, if measurement methods based on ellipses are used to detect the target (e.g. best-fit ellipses), the calculated ellipse centre does not correspond to the desired target centre in 3D space. This paper firstly discusses the use and measurement of circular and spherical targets. It then describes the geometrical projection model in order to demonstrate the eccentricity in image space. Based on numerical simulations, the eccentricity in the image is further quantified and investigated. Finally, the resulting effect in 3D space is estimated for stereo and multi-image intersections. It can be stated that the eccentricity is larger than usually assumed, and must be compensated for high-accuracy applications. Spherical targets do not show better results than circular targets. The paper is an updated version of Luhmann (2014) new experimental investigations on the effect of length measurement errors.


2012 ◽  
Vol 503-504 ◽  
pp. 1454-1457
Author(s):  
Xiang Fu Tan ◽  
Chao Xiong Xu ◽  
Rui Wen Shi ◽  
Wei Jian Guo ◽  
Tao Mao ◽  
...  

The universal tool microscope is widely applied in geometric measurement, as the imaging method is a typical measurement methods. In this paper, data processing and calculation example to specify the method of image measurement of the external thread, Classifications have been carried out systematically in this paper on the origin of error of the universal tool microscope, the image- measuring methods was summarized.


2013 ◽  
Vol 456 ◽  
pp. 299-302
Author(s):  
Sheng Huai Wang ◽  
Feng Hua Xu ◽  
Yu Rong Chen ◽  
Tie Bang Xie

The texture and characteristics of surface have been becoming the leading factors to achieve the designed function of products successfully. So the measurement of surface texture is more and more important. A kind of new multi-function fusion measurement method for surface texture is presented in the paper. Four kinds of different scale measurement methods such as microscopy image measurement, vertical scanning white light interference measurement, white light interference nanoprobe measurement and white light interference diamond-probe measurement are fused for the surface texture multi-function fusion measurement in this method.


Author(s):  
W. H. Wu ◽  
R. M. Glaeser

Spirillum serpens possesses a surface layer protein which exhibits a regular hexagonal packing of the morphological subunits. A morphological model of the structure of the protein has been proposed at a resolution of about 25 Å, in which the morphological unit might be described as having the appearance of a flared-out, hollow cylinder with six ÅspokesÅ at the flared end. In order to understand the detailed association of the macromolecules, it is necessary to do a high resolution structural analysis. Large, single layered arrays of the surface layer protein have been obtained for this purpose by means of extensive heating in high CaCl2, a procedure derived from that of Buckmire and Murray. Low dose, low temperature electron microscopy has been applied to the large arrays.As a first step, the samples were negatively stained with neutralized phosphotungstic acid, and the specimens were imaged at 40,000 magnification by use of a high resolution cold stage on a JE0L 100B. Low dose images were recorded with exposures of 7-9 electrons/Å2. The micrographs obtained (Fig. 1) were examined by use of optical diffraction (Fig. 2) to tell what areas were especially well ordered.


Author(s):  
E. Loren Buhle ◽  
Pamela Rew ◽  
Ueli Aebi

While DNA-dependent RNA polymerase represents one of the key enzymes involved in transcription and ultimately in gene expression in procaryotic and eucaryotic cells, little progress has been made towards elucidation of its 3-D structure at the molecular level over the past few years. This is mainly because to date no 3-D crystals suitable for X-ray diffraction analysis have been obtained with this rather large (MW ~500 kd) multi-subunit (α2ββ'ζ). As an alternative, we have been trying to form ordered arrays of RNA polymerase from E. coli suitable for structural analysis in the electron microscope combined with image processing. Here we report about helical polymers induced from holoenzyme (α2ββ'ζ) at low ionic strength with 5-7 mM MnCl2 (see Fig. 1a). The presence of the ζ-subunit (MW 86 kd) is required to form these polymers, since the core enzyme (α2ββ') does fail to assemble into such structures under these conditions.


Author(s):  
Paul DeCosta ◽  
Kyugon Cho ◽  
Stephen Shemlon ◽  
Heesung Jun ◽  
Stanley M. Dunn

Introduction: The analysis and interpretation of electron micrographs of cells and tissues, often requires the accurate extraction of structural networks, which either provide immediate 2D or 3D information, or from which the desired information can be inferred. The images of these structures contain lines and/or curves whose orientation, lengths, and intersections characterize the overall network.Some examples exist of studies that have been done in the analysis of networks of natural structures. In, Sebok and Roemer determine the complexity of nerve structures in an EM formed slide. Here the number of nodes that exist in the image describes how dense nerve fibers are in a particular region of the skin. Hildith proposes a network structural analysis algorithm for the automatic classification of chromosome spreads (type, relative size and orientation).


2018 ◽  
Vol 11 (4) ◽  
pp. 249-266 ◽  
Author(s):  
Judith Znanewitz ◽  
Lisa Braun ◽  
David Hensel ◽  
Claudia Fantapié Altobelli ◽  
Fabian Hattke

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