scholarly journals Salmonella Enteritidis in Broiler Chickens: Isolation, Antibiotic Resistance Phenotyping and Efficacy of Colistin on Control of Experimental Infection

Out of 400 examined samples 45 suspected Salmonella isolates (11.25%) were obtained 19 (9.5%) out of apparently healthy and 26 (13%) from diseased chickens. Intestinal samples had more isolates (29, 14.5%) more than liver (16, 8%). Identified S.Enteritidis from suspected salmonella was 16/45 (35.6%) with a rate of 8% out of the examined 400 samples, 6 (3.0%) out of apparently healthy and 10 (5.0%) from diseased chickens. Intestinal samples had more isolates (11, 5.5%) than liver (5, 2.5%). The Antibiotics susceptibility profile of S. Enteritidis isolates revealed 100% resistance to trimethoprim/sulfamethoxazole, followed by oxacillin (62.5%), 56.3% for each of ampicillin, clindamycin, enrofloxacin and doxycycline, 50% for chloramphenicol, 43.8% for streptomycin, 37.5% to cephalosporins and 18.8% for colistin. Tested S. Enteritidis isolates are classified into 11 profiles and are resistant to two - nine antibiotic classes with resistant index 0.2- 0.9. Only two isolates are NDR (12.5%), most of isolates 10/16 (62.5%) are MDR and 25% are EDR to 8-9 antibiotics. Clinical signs in experimentally infected chickens appeared at 2nd dpi, mortality started at the 4th to reach 27.5% in infected nontreated and 5% in colistin treated. Signs and lesions were markedly severe in infected nontreated than treated. S. Enteritidis was re-isolated from dead infected birds. S. Enteritidis intestinal count in sacrificed infected nontreated was higher than treated. Colistin treated group showed higher FCR, EEF and CV% (1.52, 402.8 and 6.12%) than infected non-treated (1.73, 222.6 and 14.83%). It could be concluded that S. Enteritidis is prevalent in broiler chicken flocks. Most of the isolates are MDR. Experimental infection of broiler with S. Enteritidis field isolates resulted in high mortality and the addition of colistin sulphate in drinking water controlled the infection and restores the productivity of infected broiler chickens.

2021 ◽  
Vol 883 (1) ◽  
pp. 012013
Author(s):  
B P Mahardhika ◽  
R Mutia ◽  
M Ridla

Abstract This study has been carried out to evaluate the use of drinking water-soluble probiotics as an alternative to Zinc bacitracin Antibiotic Growth Promoter (AGP) in an effort to reduce the concentration of ammonia excreta as a result of increased feed digestibility. The probiotic used contains 2.0 × 107 CFU mL−1 Lactobacillus sp, 1.6 × 107 CFU mL−1 Bacillus sp, and 7.4 × 109 CFU mL−1 Streptomyces sp. The excreta sample was obtained from 15 male Lohmann strain broiler chickens aged 35 days from a total population of 300 chickens that had previously been reared since Day Old Chick. The chickens were distributed into three treatments and five replications in a metabolic cage with a size of 50 cm × 30 cm × 56 cm for three days. This study used Completely Randomized Design (CRD) with Analysis of Variance (ANOVA). The addition of probiotic significantly reduced (P <0.01) water content and ammonia concentration of broiler chicken excreta. The addition of drinking water-soluble probiotics significantly increased (P <0.01) the feed intake and feed digestibility of broiler chicken. The use of probiotics was better than Zinc bacitracin in reducing excreta ammonia concentration and feed digestibility.


2021 ◽  
Vol 5 (1) ◽  
pp. 32-40
Author(s):  
andy andytriwibowo ◽  
◽  
Novi Eka Wati ◽  
Miki Suhadi ◽  
◽  
...  

ABSTRACT Herbal plants can be used as feed additive, on of which is the papaya plant (Carrica papaya L). The reseach was conducted on 11-27 September 2020 at street Wolter Monginsidi alley Nuri number 55, Teluk Betung North District, Bandar Lampung. This study aims to determine the effect of adding papaya leaf juice in drinking water to the performance broiler chickens. The material used was 96 broiler chickens ranging from 5-21 days of age. The method used was a Completely Randomized Design (CRD) with 4 treatments and 4 replications. The treatments given was control drinking water without additional (P0), control drinking water + 5 ml papaya leaf juice/liter of water (P1), control drinking water + 10 ml papaya leaf juice/liter of water (P2), control drinking water + 15 ml papaya leaf juice/liter of water (P3). The result showed that the addition of papaya leaf juice up to level 1,5% did not have a significant effect on the performance of broiler chickens at weeks 1, 2, and 3. Based on the reseach result it can be concluded that the addition of papaya leaf juce in drinking water up to level 1.5% has not had a significant effect (P>0,05) on performance broiler chicken. Keywords : Broiler Chicken, Papaya Leaf Juice, Drinking Water, Broiler Performance


2012 ◽  
Vol 64 (5) ◽  
pp. 1188-1193 ◽  
Author(s):  
R.C.L. Assis ◽  
M.C. Cury ◽  
F.D. Luns ◽  
R.L. Assis

The experiment was carried out with 150 Cobb broiler chickens divided into 3 groups with 50 birds each. The groups of infected chickens orally received 1ml of inoculum containing 3x103 Eimeria acervulina sporulated oocysts at 12 days of age. Group 1 was kept as a positive control with infected non-medicated birds, group 2 was medicated with diclazuril (1%) with a dose of 1mL/4 L of drinking water for 2 successive days, 5 days after infection, while group 3 was kept as negative control with non-infected and non-medicated birds. Oocysts count per gram of feces, score of macroscopic intestinal lesions and weight gain were evaluated. The group treated with diclazuril showed significant and satisfactory improvement in the assessment criteria when compared to the infected non-medicated group. The results revealed more reduction in the total oocyst count and intestinal lesion score in the medicated than in the infected non-treated group. The results confirmed that (1%) liquid diclazuril is effective to control Eimeria infection.


2014 ◽  
Vol 77 (1) ◽  
pp. 40-49 ◽  
Author(s):  
MOUSSA SORY DIARRA ◽  
PASCAL DELAQUIS ◽  
HEIDI REMPEL ◽  
SUSAN BACH ◽  
COLLEEN HARLTON ◽  
...  

The objective of this study was to analyze the antibiotic resistance phenotype and genotype of Salmonella isolated from broiler production facilities. A total of 193 Salmonella isolates recovered from commercial farms in British Columbia, Canada, were evaluated. Susceptibility to antibiotics was determined with the Sensititre system. Virulence and antibiotic resistance genes were detected by PCR assay. Genetic diversity was determined by pulse-field gel electrophoresis (PFGE) typing. Seventeen serovars of Salmonella were identified. The most prevalent Salmonella serovars were Kentucky (29.0% of isolates), Typhimurium (23.8%), Enteritidis (13.5%), and Hadar (11.9%); serovars Heidelberg, Brandenburg, and Thompson were identified in 7.7, 4.1, and 3.6% of isolates, respectively. More than 43% of the isolates were simultaneously resistant to ampicillin, amoxicillin–clavulanic acid, ceftiofur, cefoxitim, and ceftriaxone. This β-lactam resistance pattern was observed in 33 (58.9%) of the Salmonella Kentucky isolates; 2 of these isolates were also resistant to chloramphenicol, streptomycin, sulfisoxazole, and tetracycline. Genes associated with resistance to aminoglycosides (aadA1, aadA2, and strA), β-lactams (blaCMY-2, blaSHV, and blaTEM), tetracycline (tetA and tetB), and sulfonamide (sul1) were detected among corresponding resistant isolates. The invasin gene (invA) and the Salmonella plasmid virulence gene (spvC) were found in 97.9 and 25.9% of the isolates, respectively, with 33 (71.7%) of the 46 Salmonella Typhimurium isolates and 17 (65.4%) of the 26 Salmonella Enteritidis isolates carrying both invA and spvC. PGFE typing revealed that the antibiotic-resistant serovars were genetically diverse. These data confirm that broiler chickens can be colonized by genetically diverse antibiotic-resistant Salmonella isolates harboring virulence determinants. The presence of such strains is highly relevant to food safety and public health.


2021 ◽  
Vol 33 ◽  
pp. 04005
Author(s):  
Tri Untari ◽  
Okti Herawati ◽  
Marla Anggita ◽  
Widya Asmara ◽  
Agnesia Endang Tri Hastuti Wahyuni ◽  
...  

Chicken is a major source of animal protein consumption in Indonesia. The problem facing the poultry industry is the incidence of resistance which increases mortality of the chicken production. One of the causes of resistance case is the use of antibiotics in feed additives. The public understanding about the effects of the use of antibiotic growth promoters (AGP) in chickens in antibiotic resistance and the digestive tract of chicken needs to be done to avoid the impact on economic losses and health problems. This study aims to provide an understanding of the effects of the use of antibiotic growth promoters (AGP) on antibiotic resistance and the digestive tract of broiler chickens. This study was carried out at a broiler chicken farm in Sleman, Yogyakarta. Based on the histopathological result of the digestive tract of chickens that were given antibiotics as AGP, there was no inflammation occurs, but the administration of antibiotics caused antibiotic resistance in various type of antibiotics including tetracycline (90% resistance), streptomycin (60% resistance), amoxicillin (50% resistance), erythromycin (80% resistance), and no resistance for gentamycin.


Author(s):  
H.Nur Fauziyah Hasanah ◽  
Tatang Sopandi

This study aims to prove the sauerkraut liquids in the drinking water of broiler chickens to weight gain, final weight, feed consumption, feed efficiency and the amount of lactic acid bacteria intestine of broilers. A total of 24 chickens broiler DOC with an average weight of 25-35 gr/tail. Broiler chickens were randomly divided into 4 groups.. Each group was given liquids sauerkraut 0 ml/L, 0.5 ml/L, 1.0 ml/L, and 1.5 ml/L in drinking water for 35 days. The results showed the treatment liquids concentration sauerkraut 1,5 ml/L in drinking water have a significant effect (P <0,05) on body weight gain, final weight, feed intake, feed efficiency and the amount of lactic acid bacteria intestines of broiler chickens. Average of body weight gain of broilers given liquids sauerkraut 1.5 ml/L in drinking water significantly (P <0.05) higher than the weight gain of broilers concentration 0 ml/L. Average of final weight of broilers given liquids sauerkraut 1,5 ml/L in drinking water significantly (P <0.05) higher than the final weight of broilers concentration 0 ml/L. Average of consumption of broiler chicken feed given liquids sauerkraut 1,5 ml/L significantly (P <0.05) lower than the consumption of broiler chicken feed concentration of 0 ml/L. Average of FCR of broilers given liquids sauerkraut 1,5 ml/L significantly (P <0.05) lower than the FCR of broilers concentration of 0 ml/L. The average amount of lactic acid bacteria intestines of broilers given liquids sauerkraut 1.5 ml/L significantly (P <0.05) higher than the amount of lactic acid bacteria intestines of broilers concentration of 0 ml/L. On the research that use of liquids sauerkraut in drinking water 1,5 ml/L can improve feed efficiency and amount of lactic acid bacteria broiler chicken intestines of 23.5%, and 19.4%.   Keywords: Broiler chickens, sauerkraut, feed efficiency, the amount of lactic acid bacteria


Author(s):  
I Dian Kinasih ◽  
Tatang Sopandi

This study aims to prove a liquid of sauerkraut in the drinking water of broilers can lower triglyceride content, cholesterol content, and abdominal fat chicken broiler. Twenty four broiler chickens aged 1 day an average of 25-35 g/ tail. Broiler chickens were randomly divided into 4 groups and repeated 6 times. Each group was given liquid of sauerkraut in drinking water with concentrations of 0, 0.5, 1.0, 1.5 ml/L for 35 days. The results of the study treatment sauerkraut liquid in drinking water concentration of 1.0 ml/L and 1.5 ml/L have a significant effect (P <0.05) on triglyceride content, cholesterol content, and abdominal fat of broiler chickens. Giving sauerkraut liquid on triglyceride content broiler concentration of 1.0 ml/L and 1.5 ml/L significantly (P <0.05) lower triglyceride content compared with broilers without liquid of sauerkraut. Giving liquid sauerkraut on cholesterol content of broiler concentration 1.0 ml/L and 1.5 ml/L significantly (P <0.05) lower cholesterol content compared with broilers without liquid of sauerkraut. Giving liquid of sauerkraut against abdominal fat of broilers concentration of 1.0 ml/L and 1.5 ml/ L significantly (P <0.05) lower cholesterol content compared with broilers without liquid of  sauerkraut. It can be concluded that the drinking water in sauerkraut liquid for broiler chicken lower triglyceride content, cholesterol content, and abdominal fat of broiler chickens at a concentration of 1.0-1.5 ml/L respectively, still at 69.4% -79.2%, 32 , 9%, - 39.4%, and 44.7% -50.3%. The measure of sauerkraut liquid in drinking water for the purpose of reduction in triglyceride content, cholesterol content, and abdominal fat of broiler chicken meat can be used at a concentration of 1.0 to 1.5 ml/L.   Keyword: broiler chicken meat, sauerkraut liquid, triglyceride content, cholesterol content, and abdominal fat.


Author(s):  
Ibrahim Hadist

Abstrak Penelitian bertujuan untuk mengetahui sampai sejauh mana pengaruh pemberian herbal yang mengandung probiotik dalam air minum terhadap konsumsi ransum segar,konsumsi bahan kering, konsumsi energi ransum, konsumsi protein ransum, dan konsumsi serat kasar ransum pada ternak ayam broiler. Penelitian ini menggunakan 100 ekor DOC broiler yang ditempatkan secara acak kedalam 20 kandang, dan setiap kandang terdiri dari 5 ekor ayam. Penelitian menggunakan metode experimental dengan Rancangan Acak Lengkap (RAL) dengan empat perlakuan air minum dan lima ulangan. Perlakuan air minum yang digunakan yaitu air minum yang tidak mengandung probiotik 0% (R1); mengandung vitamin dan antibiotik (R2); mengandung 1 ml herbal yang mengandung probiotik (R3); dan mengandung 2 ml herbal yang mengandung probiotik (R4). Hasil penelitian menunjukan bahwa penambahan herbal yang mengandung probiotik tidak berpengatuh terhadap palatabilitas ransum ayam broiler. Kata kunci : herbal, probiotik, air minum, palatabilitas, ayam broiler Abstract The object of study was evaluate the extent of the effect of herbs that contain probiotics in drinking water on feed consumption, dry matter intake, feed energy consumption, the consumption of dietary protein, and crude fiberconsumption in broiler chickens. This reseach used 100 broiler at one days of age, were randomly placed into 20 cages, each cage consisted of five chickens. The methode of research was experimental designed by completely randomized design (CRD) with four treatments and five replications of drinking water. The treatments consisted of drinking water that does not contain probiotics 0% (R1); drinking water were contain vitamins and antibiotics (R2); drinking water were contain 1 ml herbs with probiotics (R3); drinking water were contain 2 ml herbs with probiotics (R4). The results showed that the addition of herbs that contain probiotics do not affect the feed palatability of broiler chicken. Keywords : Herb, probiotic, drinking water, palatability, broiler chicken


2018 ◽  
Vol 2 (3) ◽  
pp. 1
Author(s):  
Resa Pahlevi ◽  
Harapin Hafid ◽  
Amiluddin Indi

This study aims to know the effect of (Piper betle L.) leaves extract addition in drinking water on carcass percentage and abdominal fat of broiler chicken. Experimental material was used to this study were eighty broiler chicken SR-707. Broiler chicken was divided into twenty pen with five treatments and four replications and each pen was filled with four broiler chickens. The treatments were P1 = drinking water without betel leaves extract, P2 = drinking water + 0,5% extract of betel leaves, P3 = drinking water + 1% extract of betel leaves, P4 drinking water + 1,5% betel leaves extract, and P5 = drinking water + 2% betel leaves extract. variables were final weight, carcass percentage and abdominal fat of 5 weeks broiler chicken. The data obtained was processed and analyzed using analysis variance and continued using F-test. The result showed that addition of betel leaves extract in drinking water did not give significant (P>0,05) effect on final weight, carcass percentage and abdominal fat of broiler chicken. The conclusion was using betel leaves extract untill 2% in broiler drinking water no decreased final weight, carcass percentage and abdominal fat of broiler chicken that was slaughtered at 5 weeks of old.Key words: Final Weight, Carcass percentage, abdominal fat, Piper betle L, BroilerABSTRAKPenelitian ini dilakukan untuk mengetahui efek dari penambahan ekstrak daun sirih (Piper betle L) dalam air minum terhadap bobot akhir persentase karkas dan lemak abdominal ayam broiler. Materi yang digunakan pada penelitian ini adalah 80 ekor ayam broiler strain SR-707 yang dipelihara selama 5 minggu. Ayam penelitian dibagi dalam 20 petak kandang dengan lima perlakuan dan empat pengulangan yang masing-masing petak diisi dengan 4 ekor ayam percobaan. Air minum perlakuan yang diberikan terdiri atas: P1= kontrol, P2 (Air minum + ekstrak daun sirih 0,5%), P3(Air minum + ekstrak daun sirih 1%), P4 (Air minum + ekstrak daun sirih 1,5%), P5 (Air minum + ekstrak daun sirih 2%). Parameter yang diamati adalah bobot akhir, persentase karkas, lemak abdominal ayam broiler umur 5 minggu. Data yang diperoleh diolah dan dianalisis berdasarkan prosedur sidik ragam dan di uji dengan uji-F. Hasil penelitian menunjukkan bahwa penambahan ekstrak daun sirih dalam air minum berpengaruh tidak nyata (p>0,05) terhadap bobot akhir, persentase karkas dan lemak abdominal ayam brolier. Kesimpulan yang di peroleh dalam penelitian ini adalah bahwa penggunaan ekstrak daun sirih sampai pada level 2% dalam air minum tidak berpengaruh terhadap bobot akhir, persentase karkas dan lemak abdominal ayam broiler yang dipotong pada umur lima minggu.Kata kunci: Bobot akhir, Persentase karkas, lemak abdominal, daun sirih, broiler.


2014 ◽  
Vol 14 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Meisji Liana Sari ◽  
Fitri Nove Liya Lubis ◽  
Lia Dwi Jaya

(The Effect of Wood Vinegar Through to The Drinking Water on The Quality of Broiler Chicken Carcass)ABSTRACT. This aims of this research was to determine the percentage of carcass, commercial carcass parting and meat bone ratio (breast, thigh and wing) of broiler consumed wood vinegar. This research was assigned into used Completely Randomized Design (CRD) with 5 different treatments and each 3 replicates. The treatments were dose of wood vinegar, R0 = water + 0% wood vinegar, R1 = water + 0.25% wood vinegar, R2 = water + 0.5% wood vinegar, R3 = water + 0.75% wood vinegar, R0 = water + 1% wood vinegar. The observed parameters were percentage of carcass, commercial sliced carcass parting and meat bone ratio. The results showed that wood vinegar in the drinking water did gave not significantly (P0,05) effect to the percentage of carcass, commercial sliced carcass parting and meat bone ratio of broiler chickens. It is concluded that Liquid wood vinegar can be used for broiler chickens until 1%.


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