Osteogenic Potential of 3-D printed Multilayered Poly L-lactic Acid (PLLA) Scaffold on the Healing of Critical-sized Bone Defect in Dogs

2020 ◽  
Keyword(s):  
Bone Defect ◽  
Osteogenic Potential ◽  
Plla Scaffold ◽  
Defect Site ◽  
Orthopedic Surgeons ◽  
Critical Defect ◽  
3D Printed ◽  
Bone Trabeculae ◽  
The Right ◽  
Medical Grade

Critical sized bone defect is a major challenge for orthopedic surgeons. These defects result following any pathologic condition leading to massive bone loss. Synthetic and biological based tissue engineered biomaterials and their combinations provide a promising substitute to fill the defect site. The aim of the present study was to evaluate the osteogenic potential of 3-D printed multilayered medical grade PLLA scaffold with collagen and nano hydroxyapatite for the healing of induced critical-sized bone defect in dogs. An experimental study was conducted on 12 skeletally mature male dogs. Critical defect (25mm) was induced into the right femur of all dogs. Dogs were randomly allocated into one of the following groups (4 dogs/group). PLLA scaffold seeded with nano hydroxyapatite and collagen molded on the defect (PLLA/Collagen/nHA group), the second group; the defect seeded with collagen and nano hydroxyapatite (Collagen/nHA group), the third one was left without scaffold or additives (Sham operated group), and the operated animals were left for 12 weeks. Animals were evaluated clinically, radiographically and histopathologicaly. In (PLLA/Collagen/nHA group), all dogs showed an improvement in lameness degree from sever to apparently free from lameness. Radiography showed newly formed bone filling the defect with no inter zone. Histopathology showed more maturation of the newly formed bone in the defect site had occurred as well as defined bone trabeculae in comparing to other groups. In conclusion, 3D printed multilayered medical grade PLLA with collagen and nano hydroxyapatite provide biodegradable osteoconductive scaffold for enhancing the healing of critical sized bone defect.

Characterization of Biomaterial and Tissue Response Analysis after Implantation

2018 ◽  
Vol 930 ◽  
pp. 48-52
Author(s):  
Eliana dos Santos Câmara-Pereira ◽  
Ana Emília Holanda Rolim ◽  
Isabela Cerqueira Barreto ◽  
Laise Monteiro Campos Moraes ◽  
Lilian Campos ◽  
...  
Keyword(s):  
Repair Process ◽  
Tissue Response ◽  
Precipitation Method ◽  
Molar Ratio ◽  
Osteogenic Potential ◽  
Response Analysis ◽  
Defect Site ◽  
Critical Defect ◽  
Defect Repair

Some biomaterials can be used to promote tissue repair process. The biological substitutes (biomaterials such as hydroxyapatite beads) can be used with some advantages and purpose of mimicking responses to on-site repair of the injured bone. The objective of this study was to evaluate the osteogenic potential of the biomaterial composed of hydroxyapatite and alginate in place of the critical defect. bioceramic samples stoichiometric hydroxyapatite was produced by the precipitation method, wet method with ion molar ratio of Ca 10 (PO 4) 6 (OH) 2, in which the Ca / P ratio was equal to 1.67. The reaction conditions were favorable to the composition of a biomaterial with crystalline phase. The synthesis of the biomaterial composed of hydroxyapatite and alginate microspheres (HAAlg5%; 200 ø 425mm) was obtained from two primary solutions with the aim of, in optimal reactive conditions, to form the precipitate. After synthesis the microspheres were implanted into the defect site. The potential effects of using HAAlg5% and the application of vibratory waves in the critical defect repair were unknown and the results described in this study are promising, considering the systemic therapy and at the site of injury. The biomaterial used promoted repair the injured tissue.

scholarly journals Nb2C MXene-Functionalized Scaffolds Enables Osteosarcoma Phototherapy and Angiogenesis/Osteogenesis of Bone Defects

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Junhui Yin ◽  
Shanshan Pan ◽  
Xiang Guo ◽  
Youshui Gao ◽  
Daoyu Zhu ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Tumor ◽  
Near Infrared ◽  
Niobium Carbide ◽  
Bone Cancer ◽  
Efficient Treatment ◽  
Defect Site ◽  
3D Printed ◽  
Reparative Process ◽  
And Migration

AbstractEarly surgical resection and chemotherapy of bone cancer are commonly used in the treatment of bone tumor, but it is still highly challenging to prevent recurrence and fill the bone defect caused by the resection site. In this work, we report a rational integration of photonic-responsive two-dimensional (2D) ultrathin niobium carbide (Nb2C) MXene nanosheets (NSs) into the 3D-printed bone-mimetic scaffolds (NBGS) for osteosarcoma treatment. The integrated 2D Nb2C-MXene NSs feature specific photonic response in the second near-infrared (NIR-II) biowindow with high tissue-penetrating depth, making it highly efficient in killing bone cancer cells. Importantly, Nb-based species released by the biodegradation of Nb2C MXene can obviously promote the neogenesis and migration of blood vessels in the defect site, which can transport more oxygen, vitamins and energy around the bone defect for the reparative process, and gather more immune cells around the defect site to accelerate the degradation of NBGS. The degradation of NBGS provides sufficient space for the bone remodeling. Besides, calcium and phosphate released during the degradation of the scaffold can promote the mineralization of new bone tissue. The intrinsic multifunctionality of killing bone tumor cell and promoting angiogenesis and bone regeneration makes the engineered Nb2C MXene-integrated composite scaffolds a distinctive implanting biomaterial on the efficient treatment of bone tumor.

scholarly journals Clinical study of 3D printed personalized prosthesis in the treatment of bone defect after pelvic tumor resection

2021 ◽  
Vol 29 ◽  
pp. 163-169
Author(s):  
Lin Xu ◽  
Hao Qin ◽  
Jia Tan ◽  
Zhilin Cheng ◽  
Xiang Luo ◽  
...  
Keyword(s):  
Clinical Study ◽  
Bone Defect ◽  
Tumor Resection ◽  
Pelvic Tumor ◽  
3D Printed

scholarly journals Unprecedented tibial bone lengthening of 33.5 cm by distraction osteogenesis for the reconstruction of a subtotal tibial bone defect. A case report and literature review

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Abdulnassir Ali ◽  
Ying Ren ◽  
Chun-Hao Zhou ◽  
Jia Fang ◽  
Cheng-He Qin
Keyword(s):  
Bone Defect ◽  
Distraction Osteogenesis ◽  
Chronic Osteomyelitis ◽  
Weight Bearing ◽  
Lower Leg ◽  
Bone Lengthening ◽  
Case Presentation ◽  
Tibial Length ◽  
Tibial Bone ◽  
The Right

Abstract Background We present a case of an immense unprecedented tibial bone lengthening of 33.5 cm. The management of chronic osteomyelitis of the right tibia with subtotal tibial bone defect, talus defect and equinus ankle deformity. We demonstrate limb reconstruction by distraction osteogenesis and correction of ankle deformity with the Ilizarov technique. Limb salvage was preferred as an alternative to amputation to restore basic limb function. Case presentation A 16-year-old male patient fell and injured his right lower leg. He attempted to treat the symptoms with traditional home remedies. During 15 months of self-treating, he developed osteomyelitis of the right tibia and had lost function in his foot. Radiology revealed immense bone defect of the right tibia, including talus bone defect and equinus deformity of the calcaneus. The patient’s right tibia was non weight-bearing, had drainage sinus just below his knee and a large scar anteriorly along the entire length of the tibia. Conclusion Upon completion of treatment, the patient was able to avoid amputation of his leg with partially restored function for weight-bearing. He carried himself without assistance after 3 years of lost function in his right leg. Tibial bone distraction osteogenesis of 33.5 cm was done after 90% of the tibial length was defected. To the best of our best knowledge, this case is one of a kind to achieve distraction of tibial bone to such length.

3D-printed Poly-Lactic Co-Glycolic Acid (PLGA) scaffolds in non-critical bone defects impede bone regeneration in rabbit tibia bone

2021 ◽  
pp. 1-7
Author(s):  
Jin Xi Lim ◽  
Min He ◽  
Alphonsus Khin Sze Chong
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Volume Ratio ◽  
Bone Defects ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Rabbit Tibia ◽  
3D Printed ◽  
Critical Bone Defects

BACKGROUND: An increasing number of bone graft materials are commercially available and vary in their composition, mechanism of action, costs, and indications. OBJECTIVE: A commercially available PLGA scaffold produced using 3D printing technology has been used to promote the preservation of the alveolar socket after tooth extraction. We examined its influence on bone regeneration in long bones of New Zealand White rabbits. METHODS: 5.0-mm-diameter circular defects were created on the tibia bones of eight rabbits. Two groups were studied: (1) control group, in which the bone defects were left empty; (2) scaffold group, in which the PLGA scaffolds were implanted into the bone defect. Radiography was performed every two weeks postoperatively. After sacrifice, bone specimens were isolated and examined by micro-computed tomography and histology. RESULTS: Scaffolds were not degraded by eight weeks after surgery. Micro-computed tomography and histology showed that in the region of bone defects that was occupied by scaffolds, bone regeneration was compromised and the total bone volume/total volume ratio (BV/TV) was significantly lower. CONCLUSION: The implantation of this scaffold impedes bone regeneration in a non-critical bone defect. Implantation of bone scaffolds, if unnecessary, lead to a slower rate of fracture healing.

3D printed polycaprolactone/beta-tricalcium phosphate/magnesium peroxide oxygen releasing scaffold enhances osteogenesis and implanted BMSCs survival in repairing the large bone defect

2021 ◽  
Author(s):  
Ziyue Peng ◽  
Chengqiang Wang ◽  
Chun Liu ◽  
Haixia Xu ◽  
Yihan Wang ◽  
...  
Keyword(s):  
3D Printing ◽  
Bone Defect ◽  
Tricalcium Phosphate ◽  
Large Bone Defect ◽  
Beta Tricalcium Phosphate ◽  
Defect Area ◽  
3D Printed ◽  
Magnesium Peroxide ◽  
Large Bone ◽  
Phosphate Magnesium

Fabricate a MgO2-contained scaffold by 3D printing to improve ischemia and hypoxia in bone defect area.

scholarly journals Reconstruction of Severe Acetabular Bone Defect with 3D Printed Ti6Al4V Augment: A Finite Element Study

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Jun Fu ◽  
Ming Ni ◽  
Jiying Chen ◽  
Xiang Li ◽  
Wei Chai ◽  
...  
Keyword(s):  
Finite Element ◽  
Yield Strength ◽  
Bone Defect ◽  
Cancellous Bone ◽  
Acetabular Bone ◽  
Fea Model ◽  
Acetabular Bone Defect ◽  
3D Printed ◽  
Von Mises ◽  
Von Mises Stresses

Purpose. The purpose of this study was to establish the finite element analysis (FEA) model of acetabular bone defect reconstructed by 3D printed Ti6Al4V augment and TM augment and further to analyze the stress distribution and clinical safety of augments, screws, and bones.Methods. The FEA model of acetabular bone defect reconstructed by 3D printed Ti6Al4V augment was established by the CT data of a patient with Paprosky IIIA defect. The von Mises stresses of augments, screws, and bones were analyzed by a single-legged stance loading applied in 3 increments (500 N, 2000 N, and 3000 N).Results. The peak von Mises stresses under the maximal loading in the 3D printed augments, screws, and cortical bone were less than the yield strength of the corresponding component. However, the peak stress in the bone was greater than the yield strength of cancellous bone under walking or jogging loading. And under the same loading, the peak compressive and shear stresses in bone contact with TM augment were larger than these with 3D printed augment.Conclusions. The FEA results show that all the components will be intact under single-legged standing. However, partial cancellous bone contacted with 3D printed augment and screws will lose efficacy under walking or jogging load. So we recommend that patients can stand under full bearing, but can not walk or jog immediately after surgery.

scholarly journals Evaluation of a poly(lactic-acid) scaffold filled with poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of a segmental bone defect in a canine model

2019 ◽  
Vol 64 (No. 12) ◽  
pp. 531-538
Author(s):  
JW Yun ◽  
SY Heo ◽  
MH Lee ◽  
HB Lee
Keyword(s):  
Lactic Acid ◽  
Bone Defect ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Poly Lactic Acid ◽  
Micro Computed Tomography ◽  
Bone Parameters ◽  
3D Printed ◽  
Group 3 ◽  
Group 2

Critical-sized bone defects are a difficult problem in both human and veterinary medicine. To address this issue, synthetic graft materials have been garnering attention. Abundant in vitro studies have proven the possibilities of poly(lactic-acid) (PLA) scaffolds and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres for treating bone defects. The present study aimed at conducting an in vivo assessment of the biological performance of a three dimensional (3D)-printed PLA scaffold filled with a PLGA/HAp nanofibrous scaffold to estimate its potential applications in bone defect reconstruction surgery. Defects were created in a 20 mm-long region of the radius bone. The defects created on the right side in six Beagle dogs (n = 6) were left untreated (Group 1). The defects on the left side (n = 6) were filled with 3D-printed PLA scaffolds incorporated with PLGA/Hap nanofibres with gelatine (Group 2). The other six Beagle dog defects were made bilaterally (n = 12) and filled with the same material as that used in Group 2 along with recombinant bone morphogenetic protein 2 (rhBMP-2) (Group 3). Both the radiological and histological examinations were performed for observing the reaction of the scaffold and the bone. Micro-computed tomography (CT) was utilised for the evaluation of the bone parameters 20 weeks after the experiment. The radiological and histological results revealed that the scaffold was biodegradable and was replaced by new bone tissue. The micro-CT revealed that the bone parameters were significantly (P < 0.05) increased in Group 3. Based on these results, our study serves as a foundation for future studies on bone defect treatment using synthetic polymeric scaffolds.

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