scholarly journals Fondant Candies Enriched with Antioxidants from Aronia Berries and Grape Marc

2020 ◽  
Vol 71 (2) ◽  
pp. 74-79
Author(s):  
Ocsana Opris ◽  
Ildiko Lung ◽  
Maria-Loredana Soran ◽  
Rodica Sturza ◽  
Aliona Ghendov-Mosanu
Keyword(s):  
Dry Mass ◽  
Biologically Active ◽  
Plant Materials ◽  
Grape Marc ◽  
Physico Chemical ◽  
Microbiological Stability ◽  
Materials Used ◽  
Sonication Technique ◽  
Active Substances

Aronia berries and grape marc, a by-product of producing wine, are rich in antioxidants. The aim of this work was to study the effects of powders and extracts of aronia berries and grape marc addition on physico-chemical quality indicators, microbiological stability in vitro, and antiradical activity of fondant candies, during storage. In order to obtain the maximum antioxidant activity of the aronia berries and grape marc extracts, the Box-Behnken experimental design was used for optimization of the extraction method using sonication technique. During 35 days of storage, the dry mass fraction of all candies obtained significantly increased, which shows that the fondant candies obtained are stable over time. An important role was played by the antioxidant capacity of the plant materials used, whose chemical composition includes a number of biologically active substances which inhibit the development of microorganisms and allow stabilization of the fondant candies.

scholarly journals RESEARCH OF ANTIOXIDANT PROPERTIES OF EXTRACTS OF THE PLANTS AND THE CALLUS BIOMASS

2017 ◽  
Vol 10 (7) ◽  
pp. 182 ◽  
Author(s):  
Roksolana Konechna ◽  
Оksana Khropot ◽  
Romana Petrina ◽  
Maria Kurka ◽  
Zoriana Gubriy ◽  
...  
Keyword(s):  
Natural Habitat ◽  
Antioxidant Properties ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Plant Materials ◽  
Biomass Extract ◽  
Set Up ◽  
Definition Of ◽  
Active Substances

Objective: This study was undertaken to research of antioxidant properties of extracts of the plants and the callus biomass.Methods: The implementation of the method of cultivating the cells, tissues, and organisms in vitro for the reception of biological material of Carlina аcaulis L. (Asteraceae) is a timely issue for the medical and pharmaceutic industry, as the domestication in vitro creates the opportunity for the year-round production of the plant material as the source of biologically active substances. The seeds and plant materials (roots) of C. acaulis L. collected from natural habitat - meadows mountains Klyva Tarnychkovain Ukraine in the Carpathians. The influence of phytohormones on growth processes of plant cells is researched; the optimal conditions for the cultivation of C. acaulis L. are defined and chosen. Biomass extract is obtained and researched for the presence of biologically active substances and conducted definition of oxygen radical absorbance capacity (ORAC) of extracts of medical herbs and callus biomass of C. acaulis L.Results: C. аcaulis L. was cultivated in vitro. There was set up a scheme of sterilization of seeds with the biggest outcome of aseptic explants. There took place the investigation of the qualitative content of the extracts of callus biomass of C. acaulis L. and the detection of flavonoids and tannins. Conducted definition of ORAC of extracts of medical herbs and callus biomass of C. acaulis L. Conclusion: The obtained data showed that the investigated extracts of callus biomass and biomass of the medical herb C. acaulis L. having antioxidant activity, which makes it possible to continue research in this direction.

COMPARATIVE ANALYSIS OF BIOACTIVE SUBSTANCES CONTENT IN THE LEAVES AND FRUITS OF A ROWAN-TREE (SORBUS AUCUPARIA FRUCTUS) AND ASSESSMENT OF PROSPECTS IN MEDICINE AND PHARMACY

2020 ◽  
pp. 58-62
Author(s):  
Chernichkina A.D.
Keyword(s):  
Raw Materials ◽  
Wide Distribution ◽  
Biologically Active ◽  
Bioactive Substances ◽  
Plant Raw Materials ◽  
Mountain Ash ◽  
The Russian Federation ◽  
Materials Used ◽  
Active Substances ◽  
Pectin Substances

A large number of biologically active substances, organic acids, tannins, and pectin substances were detected during the study of fruits, leaves, and pulp of the fruits of mountain Ash. The content of these substances in leaves and pulp will significantly expand the arsenal of medicinal plant raw materials used. Given the wide distribution of mountain Ash in the territory of the Russian Federation, harvesting leaves from the same plants after harvesting the fruit and using pulp will make it possible to obtain new phytopreparations.

scholarly journals Determination of the Qualitative Composition of Biologically Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhaponticum carthamoides

2021 ◽  
Vol 11 (6) ◽  
pp. 2555
Author(s):  
Lyudmila Asyakina ◽  
Svetlana Ivanova ◽  
Alexander Prosekov ◽  
Lyubov Dyshlyuk ◽  
Evgeny Chupakhin ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cell Suspension ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Root Cultures ◽  
Qualitative Composition ◽  
Callus Cell ◽  
Rhaponticum Carthamoides ◽  
Active Substances

This work aims to study the qualitative composition of biologically active substance (BAS) extracts in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides. The research methodology is based on high-performance liquid chromatography, and 1H nuclear magnetic resonance (NMR) spectra, to study the qualitative and quantitative analysis of BAS. The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension and root cultures showed that the main biologically active substances in the medicinal plant Rhaponticum carthamoides are 2-deoxy-5,20,26-trihydroxyecdyson (7 mg, yield 0.12%), 5,20,26-trihydroxyecdyson 20,22-acetonide (15 mg, yield 0.25%), 2-deoxy-5,20,26-trihydroxyecdyson 20,22-acetonide (6 mg, yield 0.10%), 20,26-dihydroxyecdyson 20,22-acetonidecdyson 20,22-acetonide (5 mg, yield 0.09%), and ecdyson 20,22-acetonide (6 mg, yield 0.10%). In the future, it is planned to study the antimicrobial, antioxidant, and antitumor activity of BAS of extracts of in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides, for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial and antioxidant effects.

scholarly journals Proliferasi Kalus dari Eksplan Hipokotil dan Kotiledon Tanaman Jarak Pagar (Jatropha curcas L.) pada Pemberian 2,4-D

2012 ◽  
Vol 14 (1) ◽  
pp. 19 ◽  
Author(s):  
Zulkarnain Zulkarnain ◽  
Lizawati Lizawati
Keyword(s):  
Light Intensity ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Compact Structure ◽  
Plant Materials ◽  
Cotyledon Explants ◽  
Light Yellow ◽  
Materials Used ◽  
Embryogenic Callus Formation

The aim of this study was to develop an efficient method for the induction of embryogenic callus formation for in vitro propagation ofjatropha. Plant materials used were 30-days old in vitro seedlings, cut into hypocotyl and cotyledon (lower, middle and upper) sections.Medium used was MS composition supplemented with vitamins, 3% sucrose, 0.7% agar at pH 5.8 ± 1, and 2,4-D (0, 1, 2, 3, 4 dan5 mg l-1). Cultures were kept at temperature of 25 ± 1 0C with 50 μmol m-2 s-1 light intensity and 16-h photoperiod. The results indicated thatthe rate of callus formation depended on the source of explant, the application of 2,4-D, and the interaction of both. The fastest callusproliferation (2.33 days following initiation) was obtained on cotyledon explants cultured on medium without 2,4-D. The explant sourcesand 2,4-D concentrations also showed significant effect on the percentage of explant forming callus. The most callus formation (88.33%)was obtained on middle cotyledon cultured on 3 mg l-1 2,4-D, whereas the fewest (6.84%) was found on upper cotyledon cultured on mediumwithout 2,4-D. The colour of callus was dominated by white, light yellow, cream and brown with mostly compact structure, particularly onhypocotyl cultured on medium without 2,4-D. The texture of callus formed on hypocotyl treated with up to 4 mg l -1 2,4-D was dominatedby coarse appearance. In contrast, majority of callus proliferated on hypocotyl treated with 5 mg l -1 2,4-D or cotyledon treated with orwithout 2,4-D produced callus with smooth texture %.

scholarly journals Assessment of the quality of peppermint leaves by thin-layer chromatography in some objects of plant origin

2020 ◽  
Vol 19 (5-6) ◽  
pp. 148-154
Author(s):  
Vali A. Sahratov ◽  
Tamara L. Malkova ◽  
Ludmila N. Karpova ◽  
Anna A. Pospelova
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Biologically Active ◽  
Mentha Piperita ◽  
Plant Origin ◽  
Plant Materials ◽  
Layer Chromatography ◽  
Active Substances

The State Pharmacopoeia of the XIV edition defines the approach for the assessment of the quality of medicinal plant materials, it deals with the identification of the main groups of biologically active substances by thin layer chromatography. According to this approach, the analysis of some types of medicinal plant materials as a part of some objects of plant origin was carried out. The article presents the quality assessment algorithm as examplified by peppermint leaves (Mentha piperita L.), which are part of a variety of plant object.

RESOURCE-SAVING TECHNOLOGY FOR PROCESSING MEDICINAL SAGE

2020 ◽  
Author(s):  
Т.В. ПЕЛИПЕНКО
Keyword(s):  
Essential Oil ◽  
Raw Materials ◽  
Total Yield ◽  
Dry Mass ◽  
Biologically Active ◽  
Flow Diagram ◽  
Resource Saving ◽  
Process Flow Diagram ◽  
Full Flowering ◽  
Active Substances

Исследованы потенциальные возможности ресурсосберегающей технологии переработки шалфея лекарственного (ШЛ). Объектом исследования были образцы эфирномасличного сырья – свежеубранные надземные части ШЛ в фазе полного цветения растения и сухие листья ШЛ. Предложенная схема переработки включает следующие стадии: получение эфирного масла; извлечение конкрета из обезэфиренных отходов экстракцией углеводородным растворителем и последующей его отгонкой; получение абсолютного масла и очищенных восков из конкрета отходов; извлечение дитерпеновых соединений экстракцией этиловым спиртом из отходов сырья после обработки гексаном. В результате исследований фракционного состава сырья и особенностей локализации эфирного масла установлено, что массовая доля эфирного масла составляет, % к сухой массе исследуемых фракций: в листьях и соцветиях 2,75, в стеблях 0,31. Рекомендовано введение ограничительных норм по соотношению технически ценной фракции – листьев и соцветий и балластной – стеблей. Полученное из ШЛ эфирное масло имеет высокое содержание туйонов (31,50%), камфоры (21,0%) и 1,8-цинеола (18,80%). Выход дитерпеновых соединений при комплексной переработке эфирномасличного сырья ШЛ на 38,0% ниже, чем из сухих листьев растения. Однако при расчете на сухую массу листьев и соцветий, составляющих 62,3% от массы сырья, показатели отличаются незначительно. Общий выход продуктов, содержащих комплекс биологически активных веществ, составил 15,53 %, что подтверждает целесообразность использования комплексной переработки шалфея лекарственного. The potential of resource-saving technology for processing medicinal sage (MS) has been studied. Samples of essential oil raw materials-freshly harvested aboveground parts of MS in the phase of full flowering of the plant and dry leaves of MS were the object of research. The proposed process flow diagram includes the following stages: obtaining essential oil; recovering a particular from deester waste by extraction with a hydrocarbon solvent and its subsequent distillation; obtaining absolute oil and refined waxes from a specific waste; extraction of diterpene compounds from raw waste after treatment with hexane by extraction with ethyl alcohol. As a result of studies of the fractional composition of raw materials and the features of localization of essential oil, it was found that the mass fraction of essential oil is, % of the dry mass of the studied fractions: 2,75 in leaves and inflorescences, and 0,31 in stems. The introduction of restrictive norms on the ratio of technically valuable fraction – leaves and inflorescences and ballast – stems is recommended. The essential oil obtained from MS has a high content of tuyons (31,50%), camphor (21,0%) and 1,8-cineol (18,80%). The yield of diterpene compounds in the complex processing of essential oil raw materials MS is 38,0% lower than from dry leaves of the plant. However when calculating the dry mass of leaves and inflorescences, which make up 62,3% of the mass of raw materials, the indicators differ slightly. The total yield products containing a complex of biologically active substances amounted to 15,53%, which confirms the feasibility of complex processing of medicinal sage.

STUDY OF BIOLOGICALLY ACTIVE SUBSTANCES OF COMPLEX CO2-EXTRACT OF ROSE HIPS AND HAWTHORN AS AN INGREDIENT OF COSMETIC PRODUCTS

2021 ◽  
Author(s):  
Т.В. ПЕЛИПЕНКО ◽  
Н.М. АГЕЕВА ◽  
Т.И. ТИМОФЕЕНКО ◽  
В.И. АБАКУМОВ ◽  
А.С. ТЕСЛЕНКО
Keyword(s):  
Peroxide Value ◽  
Unsaturated Fatty Acids ◽  
Acid Value ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Cosmetic Products ◽  
Physico Chemical ◽  
Three Samples ◽  
Normative Documentation ◽  
Active Substances

Показана необходимость обеспечения нормативной документацией СО2-экстрактов с целью повышения объективности оценки их качества. Объектом исследований были три образца комплексного СО2-экстракта плодов шиповника и боярышника (фирма «Явента», г. Краснодар). Процентное соотношение масс плодов шиповника и боярышника в исходном сырье составило соответственно 70 : 30. Определены основные органолептические и физико-химические показатели комплексного СО2-экстракта плодов шиповника и боярышника: кислотное число, эфирное число, перекисное число, массовая доля эфирного масла. Величина показателя перекисного числа образца комплексного СО2-экстракта плодов шиповника и боярышника составила 6,8 мэкв/кг и может служить идентификационным признаком подлинности экстракта. Установлено наличие в составе исследованного комплексного СО2-экстракта плодов шиповника и боярышника биологически активных веществ: каротиноидов, токоферолов, ненасыщенных жирных кислот, флавоноидов, терпеновых соединений, подтверждающих целесообразность его использования в рецептурах косметических средств. The necessity to provide normative documentation of CO2-extracts with the aim of increasing the objectivity of assessment of their quality is shown. Three samples of complex CO2-extract of rose hips and hawthorn (firma “Yaventa”, Krasnodar) have been the object of research. The percentage masses of rose hips and hawthorn in the feedstock was accordingly 70 : 30. Main organoleptic and physico-chemical characteristics of complex CO2-extract of rose hips and hawthorn – acid value, ester value, peroxide value, mass fraction of essential oil determined. Values of peroxide value of a sample of complex CO2-extract of rose hips and hawthorn amounted to 6,8 mEq/kg and may serve as identification of the authenticity of the extract. The presence of studied complex CO2-extract of rose hips and hawthorn of biologically active substances: carotenoids, tocopherols, unsaturated fatty acids, flavonoids, terpene compounds, confirming the feasibility of its use in formulations of cosmetic products, was installed.

scholarly journals Animal Models as Tools to Investigate Antidiabetic and Anti-Inflammatory Plants

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Mohamed Eddouks ◽  
Debprasad Chattopadhyay ◽  
Naoufel Ali Zeggwagh
Keyword(s):  
Animal Models ◽  
Public Health Problem ◽  
New Drugs ◽  
Inflammatory Activity ◽  
Global Public Health ◽  
Plant Materials ◽  
Inflammatory Mechanism ◽  
Anti Inflammatory ◽  
Materials Used

Plants have been historically used for diabetes treatment and related anti-inflammatory activity throughout the world; few of them have been validated by scientific criteria. Recently, a large diversity of animal models has been developed for better understanding the pathogenesis of diabetes mellitus and its underlying inflammatory mechanism and new drugs have been introduced in the market to treat this disease. The aim of this work is to review the available animal models of diabetes and anti-inflammatory activity along with somein vitromodels which have been used as tools to investigate the mechanism of action of drugs with potential antidiabetic properties and related anti-inflammatory mechanism. At present, the rigorous procedures for evaluation of conventional antidiabetic medicines have rarely been applied to test raw plant materials used as traditional treatments for diabetes; and natural products, mainly derived from plants, have been tested in chemically induced diabetes model. This paper contributes to design new strategies for the development of novel antidiabetic drugs and its related inflammatory activity in order to treat this serious condition which represents a global public health problem.

scholarly journals VARIABILITY OF THE CONTENT OF FLAVONOIDS AND POLYSACCHARIDES OF BIDENS CERNUA HERB.

2020 ◽  
pp. 197-206
Author(s):  
Natal'ya Valer'yevna Karazhan
Keyword(s):  
Biologically Active Substances ◽  
Biologically Active ◽  
Plant Materials ◽  
Mass Flowering ◽  
Drying Temperature ◽  
Polysaccharide Content ◽  
Raw Plant Materials ◽  
Drying Conditions ◽  
Active Substances ◽  
Natural Drying

The aim of the research was to study the variability of the content of polysaccharides and flavonoids of Bidens cernua herb, depending on the terms of harvesting, drying conditions and place of growth on the territory of the Republic of Belarus. It was shown that the maximum polysaccharide content was observed in the phase of budding and mass flowering of the plant. Unlike polysaccharides, the content of flavonoids in the budding phase was significantly lower than in the mass flowering phase (p<0.05), which accounts for the maximum content of flavonoids, and in particular, luteolin-7-O-glucoside. The highest polysaccharide content was noted for raw plant materials undergone natural drying or at 40 and 60 °C without ventilation. An increase in the drying temperature or the use of ventilation during drying led to a significant decrease in the content of this group of biologically active substances. The content of flavonoids of Bidens cernua herb, dried at elevated temperature with ventilation, regardless of the drying temperature used, was higher than the content of this group of biologically active substances in comparison with samples of raw plant materials subjected to natural drying (p<0.05). A higher flavonoid content was also noted for Bidens cernua herb, dried without ventilation at 40 and 60 °C. The content of polysaccharides and flavonoids of Bidens cernua herb, depending on the harvesting region, varied between 35.64–90.52 mg/g and 23.11–49.86 mg/g, respectively, and was most dependent on the amount of precipitation per year region. Based on the results obtained, it is recommended to harvest Bidens cernua herb during mass flowering and to dry at 40 °C without ventilation.

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