scholarly journals Determination of Porphyrin-Induced Peroxidation of Plant Lipids by Oxygen Electrode.

2000 ◽  
Vol 45 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Mototsugu Yanagida ◽  
Hiroshi Matsumoto ◽  
Kenji Usui
1978 ◽  
Vol 43 (3) ◽  
pp. 713-718 ◽  
Author(s):  
Jaroslav Votruba ◽  
Miroslav Sobotka ◽  
Aleš Prokop

1989 ◽  
Vol 72 (1) ◽  
pp. 30-33
Author(s):  
Vicki J Smith ◽  
Richard A Green ◽  
Thomas R Hopkins

Abstract A new method for the determination of the artificial sweetener aspartame is described. α-Chymotrypsin is used to cleave the methyl ester group of aspartame, producing methanol hydrolytically. The methanol is detected using an electrode which is constructed by physically trapping yeast alcohol oxidase enzyme at the tip of a dissolved oxygen electrode. The decrease in oxygen concentration, which occurs as methanol is enzymatically oxidized to formaldehyde, is measured amperometrically. Aspartame levels in diet soft drinks as determined by the proposed method and by liquid chromatography are in excellent agreement. The relative standard deviation of the measurements is 0.83%. The methanol present in diet cola as a result of aspartame degradation can also be measured by using the electrode without α-chymotrypsin.


1987 ◽  
Vol 33 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Mikio Chiba ◽  
Alan W. Bown ◽  
David Danic

The inhibition of yeast (Saccharomyces cerevesiae) metabolism by fungicidal chemicals was investigated. Glucose- or ethanol-dependent yeast respiration was measured with an oxygen electrode, and manometric determination of carbon dioxide release was used to measure fermentation. Both respiration and fermentation were inhibited more by benomyl than by identical molar concentrations of its breakdown product, carbendazim. Butyl isocyanate, another benomyl breakdown product, inhibited respiration more but inhibited fermentation less than the parent compound. Of the isocyanates tested, hexyl isocyanate was the most inhibitory towards both activities. Captan was more active and iprodione less active than benomyl. Because benomyl rapidly broke down to carbendazim when it was prepared in 80% ethanol, only 59% of the dissolved benomyl was intact when it was added to yeast to determine its effect on respiration or fermentation.


2000 ◽  
Vol 12 (4) ◽  
pp. 292-295 ◽  
Author(s):  
Johanna Klemm ◽  
Mamas I. Prodromidis ◽  
Miltiades I. Karayannis
Keyword(s):  

1981 ◽  
Vol 46 (12) ◽  
pp. 3057-3062 ◽  
Author(s):  
Petr Sušinka ◽  
Milica Miadoková

Parallel to kinetic measurements, the potential of copper was monitored on the (100), (110), and (111) crystallographic planes during its spontaneous reaction in sodium hydroxide in oxygen atmosphere. A quantitative relation was obtained for the potential in dependence on the experimental conditions, indicating that unless a passivating layer of copper(II) oxide is formed on the surface, the metal does not act as an oxygen electrode. The potential change in the given medium was found to depend only on the amount of copper(I) oxide formed, obeying the relation E = (RT/nF) ln (mCu2O) + K; use was made of this relation for a quantitative determination of the growth of the copper(I) oxide layer.


1995 ◽  
Vol 41 (4-5) ◽  
pp. 366-371 ◽  
Author(s):  
Izumi Kubo ◽  
Isao Karube ◽  
Toshifumi Takeuchi ◽  
Masako Furusawa ◽  
Yoshiko Arikawa ◽  
...  

A biosensor based on Thiobacillus thioparus was developed for the determination of thiosulfate and methanethiol. Thiobacillus thioparus is a chemoautotrophic bacterium and it oxidizes sulfur compounds to sulfuric acid. The sensor consisted of an oxygen electrode and immobilized T. thioparus. When the sensor was used to determine thiosulfate, a linear relation between sensor output and concentration was obtained for the concentration range from 1 to 100 μM in a batch system and from 1 to 10 mM in a flow injection system. Output of the sensor was stable for more than 1 month. For methanethiol, the response of the sensor was measured for the concentration range from 0.2 to 3 mM in a flow injection system.Key words: microbial sensor, thiosulfate, methanethiol, Thiobacillus thioparus TK-m.


Sign in / Sign up

Export Citation Format

Share Document