Determination of aeration capacity of bubbled column type fermentors with oxygen electrode

1978 ◽  
Vol 43 (3) ◽  
pp. 713-718 ◽  
Author(s):  
Jaroslav Votruba ◽  
Miroslav Sobotka ◽  
Aleš Prokop
Keyword(s):  
Oxygen Electrode ◽  
Column Type ◽  
Aeration Capacity

Evaluation of aeration capacity of fermentors by adaptive identification method

1980 ◽  
Vol 45 (8) ◽  
pp. 2266-2271 ◽  
Author(s):  
Miroslav Sobotka ◽  
Jaroslav Votruba
Keyword(s):  
Time Dependence ◽  
Oxygen Electrode ◽  
New Method ◽  
Dynamic Experiment ◽  
Adaptive Identification ◽  
Identification Method ◽  
Column Type ◽  
Method Of Evaluation ◽  
Aeration Capacity

A new method of evaluation of the aeration capacity, kLa from data measured with the use of an oxygen electrode was proposed, based on the algorithm of adaptive identification. In this way, even the time dependence of kLa in the course of a dynamic experiment can be obtained. The results from mechanically stirred fermentors and apparatuses of the bubbled column type show that the commonly used assumption about the time independence of kLa does not hold for intensively aerated apparatuses.

Determination of the aeration capacity of bubble columns by dynamic method. The influence of axial dispersion and of the start-up period

1979 ◽  
Vol 44 (9) ◽  
pp. 2583-2597 ◽  
Author(s):  
Václav Linek ◽  
Jiří Stejskal ◽  
Jiří Sinkule ◽  
Václav Vacek
Keyword(s):  
Dynamic Method ◽  
Dispersion Model ◽  
Oxygen Electrode ◽  
Axial Dispersion ◽  
Bubble Columns ◽  
Oxygen Probe ◽  
Start Up ◽  
Axial Dispersion Model ◽  
Aeration Capacity

A dynamic method for the determination of volumetric mass transfer coefficient of oxygen, kLa, in bubble columns using an oxygen electrode was derived on the basis of a liquid phase axial dispersion model The influence of aeration startup was studied assuming that the kLa value is position and time dependent. The conditions are defined under which the influence of aeration startup and of axial mixing of the liquid upon the steady state kLa value is negligible. A critical assessment has been made of various methods proposed for evaluation of oxygen probe responses.

Determination of Aspartame in Beverages Using an Alcohol Oxidase Enzyme Electrode

1989 ◽  
Vol 72 (1) ◽  
pp. 30-33
Author(s):  
Vicki J Smith ◽  
Richard A Green ◽  
Thomas R Hopkins
Keyword(s):  
Liquid Chromatography ◽  
Standard Deviation ◽  
Methyl Ester ◽  
Alcohol Oxidase ◽  
Ester Group ◽  
Oxygen Electrode ◽  
Artificial Sweetener ◽  
Relative Standard ◽  
Oxidase Enzyme

Abstract A new method for the determination of the artificial sweetener aspartame is described. α-Chymotrypsin is used to cleave the methyl ester group of aspartame, producing methanol hydrolytically. The methanol is detected using an electrode which is constructed by physically trapping yeast alcohol oxidase enzyme at the tip of a dissolved oxygen electrode. The decrease in oxygen concentration, which occurs as methanol is enzymatically oxidized to formaldehyde, is measured amperometrically. Aspartame levels in diet soft drinks as determined by the proposed method and by liquid chromatography are in excellent agreement. The relative standard deviation of the measurements is 0.83%. The methanol present in diet cola as a result of aspartame degradation can also be measured by using the electrode without α-chymotrypsin.

Inhibition of yeast respiration and fermentation by benomyl, carbendazim, isocyanates, and other fungicidal chemicals

1987 ◽  
Vol 33 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Mikio Chiba ◽  
Alan W. Bown ◽  
David Danic
Keyword(s):  
Carbon Dioxide ◽  
Parent Compound ◽  
Oxygen Electrode ◽  
Breakdown Product ◽  
Carbon Dioxide Release ◽  
Saccharomyces Cerevesiae ◽  
Manometric Determination

The inhibition of yeast (Saccharomyces cerevesiae) metabolism by fungicidal chemicals was investigated. Glucose- or ethanol-dependent yeast respiration was measured with an oxygen electrode, and manometric determination of carbon dioxide release was used to measure fermentation. Both respiration and fermentation were inhibited more by benomyl than by identical molar concentrations of its breakdown product, carbendazim. Butyl isocyanate, another benomyl breakdown product, inhibited respiration more but inhibited fermentation less than the parent compound. Of the isocyanates tested, hexyl isocyanate was the most inhibitory towards both activities. Captan was more active and iprodione less active than benomyl. Because benomyl rapidly broke down to carbendazim when it was prepared in 80% ethanol, only 59% of the dissolved benomyl was intact when it was added to yeast to determine its effect on respiration or fermentation.

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

2000 ◽  
Vol 12 (4) ◽  
pp. 292-295 ◽  
Author(s):  
Johanna Klemm ◽  
Mamas I. Prodromidis ◽  
Miltiades I. Karayannis
Keyword(s):  
Oxygen Electrode

scholarly journals Determination of Porphyrin-Induced Peroxidation of Plant Lipids by Oxygen Electrode.

2000 ◽  
Vol 45 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Mototsugu Yanagida ◽  
Hiroshi Matsumoto ◽  
Kenji Usui
Keyword(s):  
Oxygen Electrode ◽  
Plant Lipids
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