Sugar beet silage as highly flexible feedstock for on demand biogas production

2018 ◽  
pp. 691-698 ◽  
Author(s):  
Kerstin Maurus ◽  
Sharif Ahmed ◽  
Wiebke Getz ◽  
Marian Kazda

On demand biogas production is a great option to complement solar and wind power for the energy revolution. Alternatives like feedstock management are important in order to avoid expensive and complex adjustments for gas storage systems. The use of sugar beet silage (S) is a good option because it mainly contains carbohydrates that are easily degradable. Anaerobic digestion was performed for 63 days in four completely stirred tank reactors (CSTR) with different ratios of maize silage (M) and S. M given every hour was used as a base load for the fermentation and S was given two times a day every 12h. Biogas and methane production rates were measured every 5min in order to achieve data with high resolution. Also, pH value, VFA/TIC values and volatile fatty acids were measured during the experiment. The process remained stable in CSTR1 (M:S1:0), CSTR2 (M:S6:1) and CSTR3 (M:S3:1). Instabilities occurred in CSTR4 (M:S1:3) after an operation time of 33 days. Nevertheless, methane yields more than doubled for CSTR3 within 5min after the input of S. Use of sugar beet as a feedstock for biogas production is a further application for this agricultural commodity.

2019 ◽  
Vol 9 (9) ◽  
pp. 1791 ◽  
Author(s):  
Xiomara Gómez-Quiroga ◽  
Kaoutar Aboudi ◽  
Carlos José Álvarez-Gallego ◽  
Luis Isidoro Romero-García

In this paper, the viability of thermophilic anaerobic co-digestion of exhausted sugar beet pulp (ESBP) and pig manure (PM) was evaluated. The effect of the proportion of ESBP on biogas production was investigated by using a series of lab-scale batch assays, in duplicates. The following five ESBP:PM mixture ratios were studied: 0:100, 10:90, 25:75, 50:50, and 100:0. The highest cumulative methane production (212.4 mL CH4/g VSadded) was reached for the mixture 25:75. The experimental results showed that the increase in the proportion of ESBP in the mixture led to the distortion of the process, due to acidification by the volatile fatty acids generated. Acetic acid was the predominant acid in all the cases, representing more than 78% of the total acidity. Moreover, the results obtained by operating at thermophilic temperatures have been compared with those obtained in a previous study conducted at mesophilic temperatures. The results have shown that in the individual digestion of ESBP, the activity of acetoclastic methanogens was affected in both temperatures, but especially in thermophilic conditions. Thus, the methane produced in the individual thermophilic digestion of ESBP came almost entirely from the activity of hydrogen-utilizing methanogenic archaea.


1980 ◽  
Vol 43 (1) ◽  
pp. 229-234 ◽  
Author(s):  
G. Kulasek ◽  
W. Barej ◽  
Hanna Leontowicz ◽  
Barbara Krasicka ◽  
M. Chomyszyn ◽  
...  

1. The purpose of the experiments was to determine certain properties of the metabolism of nitrogen compounds and carbohydrates in the rumen and tissues of growing bulls which were given either a diet containing sugar-beet silage, a urea-mineral preparation and hay or a control diet with maize silage, ground barley and hay. Daily rations were given in two equal portions twice daily at 07.00 hours and 13.00 hours. The experiment lasted 182 d.2. The experimental diet containing 0.54 g urea and 5.0 g saccharose/kg body-weight did not cause any symptoms of toxicity in the animals although there were large changes in the concentration of metabolites in the forestomach. The lowest pH (6.2–6.4) in the rumen of animals in the experimental group was observed 1.5 h after feeding. At the same time the highest level of lactic acid was observed, but the highest level of volatile fatty acids (VFA) was observed 3 h after feeding. Although there was a large increase in ammonia in the rumen contents (approximately 20 mmol/l at 1.5 h after feeding) an increase in the blood level of ammonia was not observed.3. A lower level of acetic acid and higher level of butyric acid and valeric acid were observed in the rumen of animals given sugar-beet silage than in animals given maize silage and ground barley. Non-glucogenic ratio of VFA in the rumen of bulls after feeding the experimental diet was approximately 3.5 while on the control diet the value was considerably higher.4. The causes of the low concentration of glucose in the blood and changes in other metabolites in the blood of experimental animals are discussed.5. Average daily gains were higher (P ≤ 0.05) in both groups of bulls given a diet containing sugar-beet silage and NPN (1087 and 1043 g/d) than with the control diet (887 g/d). Changes in the concentration of metabolites in the rumen contents and blood of sugar-beet-silage-fed bulls confirmed the possibility of effective addition of urea in an amount corresponding to 50% of the N content and also indicated good utilization of the energy in this diet.


Anaerobe ◽  
2017 ◽  
Vol 46 ◽  
pp. 114-121 ◽  
Author(s):  
Sharif Ahmed ◽  
Marian Kazda

2020 ◽  
Vol 8 (2) ◽  
pp. 169 ◽  
Author(s):  
Johanna Klang ◽  
Ulrich Szewzyk ◽  
Daniel Bock ◽  
Susanne Theuerl

In this study the response of biogas-producing microbiomes to a profound feedstock change was investigated. The microbiomes were adapted to the digestion of either 100% sugar beet, maize silage, or of the silages with elevated amounts of total ammonium nitrogen (TAN) by adding ammonium carbonate or animal manure. The feedstock exchange resulted in a short-range decrease or increase in the biogas yields according to the level of chemical feedstock complexity. Fifteen taxa were found in all reactors and can be considered as generalists. Thirteen taxa were detected in the reactors operated with low TAN and six in the reactors with high TAN concentration. Taxa assigned to the phylum Bacteroidetes and to the order Spirochaetales increased with the exchange to sugar beet silage, indicating an affinity to easily degradable compounds. The recorded TAN-sensitive taxa (phylum Cloacimonetes) showed no specific affinity to maize or sugar beet silage. The archaeal community remained unchanged. The reported findings showed a smooth adaptation of the microbial communities, without a profound negative impact on the overall biogas production indicating that the two feedstocks, sugar beet and maize silage, potentially do not contain chemical compounds that are difficult to handle during anaerobic digestion.


2020 ◽  
Vol 40 (1) ◽  
pp. 133-143
Author(s):  
T. A. Amole ◽  
B. O. Oduguwa ◽  
O. S. Onifade ◽  
O. M. Arigbede ◽  
A. O. Jolaosho

Silages of sole maize and mixtures of maize-lablab were made at harvest in plastic bags. The experimental Unit consists of twelve cross-bred yearling bulls (White Fulani x N’dama) calves weighing 71-72kg and were randomly allotted to three dietary treatments: Grazing + sole maize silage, Grazing + mixtures of maize-lablab silage and Unsupplemented grazing as control. The experimental design was a completely randomized design and lasted 84days. Silage diets were given between 07:30 and 11:00 hours daily before grazing. Rumen fluid was collected at the end of the study to evaluate the co-efficient of ruminal fluid. The crude protein content in sole maize silage was half of that in mixture of maize-lablab silage. Inclusion of lablab in maize stover silage increased the mineral content as well as the lignin fraction. The mean pH value of sole maize silage (3.80) was less (P < 0.05) than 4.25 obtained with addition of lablab. The buffering capacity (5.37%) of sole maize silage significantly (P < 0.05) increased to 5.71% in mixture of maize-lablab silage. Lactic acid contents differed was influenced significantly (P < 0.05) with a mean value of 8.4% and 8.5% respectively in the sole maize silage and with lablab inclusion. Total volatile fatty acid (VFA) ranged from 56.7 μmoles/ml in calves without supplementation to 95.2 μmoles/ml in the calves fed mixture of maize-lablab silage. Mixture of Maize-lablab silage had the highest values for all the minerals determined in the feed materials while sole maize had the least. It was concluded from this trial, that forage legumes are relatively good sources of degradable nitrogen and fermentable energy, so their inclusion in the diet is likely to increase the rumen population of cellulolytic microbes and also proves that, mixture of maize-lablab silage could be used as supplement to enhance the growth and survival of calves during the dry season, when animal feeds are relatively scarce.


2021 ◽  
Vol 12 ◽  
Author(s):  
Jie Xu ◽  
Hongjian Lin ◽  
Kuichuan Sheng

Proper disposal and utilization of dead pig carcasses are problems of public concern. The combination of hydrothermal pretreatment (HTP) and anaerobic digestion is a promising method to treat these wastes, provided that digestion inhibition is reduced. For this reason, the aim of this work was to investigate the optimal HTP temperature (140–180°C) for biogas production during anaerobic digestion of dead pigs in batch systems. In addition, the effects of hydrochar addition (6 g/L) on anaerobic digestion of pork products after HTP in continuous stirred tank reactors (CSTR) were determined. According to the results, 90% of lipids and 10% of proteins present in the pork were decomposed by HTP. In addition, the highest chemical oxygen demand (COD) concentration in liquid products (LP) reached 192.6 g/L, and it was obtained after 170°C HTP. The biogas potential from the solid residue (SR) and LP was up to 478 mL/g-VS and 398 mL/g-COD, respectively. A temperature of 170°C was suitable for pork HTP, which promoted the practical biogas yield because of the synergistic effect between proteins and lipids. Ammonia inhibition was reduced by the addition of hydrochar to the CSTR during co-digestion of SR and LP, maximum ammonia concentration tolerated by methanogens increased from 2.68 to 3.38 g/L. This improved total biogas yield and degradation rate of substrates, reaching values of 28.62 and 36.06%, respectively. The acetate content in volatile fatty acids (VFA) may be used as an index that reflects the degree of methanogenesis of the system. The results of the present work may also provide guidance for the digestion of feedstock with high protein and lipid content.


Processes ◽  
2019 ◽  
Vol 7 (9) ◽  
pp. 635 ◽  
Author(s):  
Siciliano ◽  
Limonti ◽  
Curcio ◽  
Calabrò

The composting process of organic fraction of municipal solid waste, besides to the residual compost, generates a wastewater that is characterized by a high organic load. The application of anaerobic processes represents an advantageous solution for the treatment and valorization of this type of wastewater. Nevertheless, few works have been focused on the anaerobic digestion of compost leachate. To overcome this dearth, in the present paper an extensive experimental investigation was carried out to develop and analyse the anaerobic treatment of young leachate in completely stirred tank reactors (CSTR). Initially, it was defined a suitable leachate pretreatment to correct its acidic characteristics that is potentially able to inhibit methanogenic biomass activity. The pretreated leachate was fed to the digester over the start-up phase that was completed in about 40 days. During the operational period, the organic load rate (OLR) changed between 4.25 kgCOD/m3d and 38.5 kgCOD/m3d. The chemical oxygen demand (COD) abatement was higher than 90% for OLR values up to 14.5 kgCOD/m3d and around to 80% for applied loads equal to 24.5 kgCOD/m3d. At this OLR, it was reached the maximum daily biogas production of about 9.3 Lbiogas/(Lreactord). The CH4 fraction was between 70%–78% and the methane production yield in the range 0.34–0.38 LCH4/gCODremoved. The deterioration of biogas production started for OLR values that were over the threshold of 24.5 kgCOD/m3d when a volatile fatty acids (VFA) accumulation occurred and the pH dropped below 6.5. The maximum ratio between VFA and alkalinity (ALK) tolerable in the CSTR was identified to be 0.5 gCH3COOH/gCaCO3. Through an economic analysis, it was proven that the digestion of compost leachate could ensure significant economic profits. Furthermore, the produced digestate had characteristics that were compatible for agricultural applications.


Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3496
Author(s):  
Mohamed K. Mohsen ◽  
Mohamed F. Ali ◽  
Hamed M. Gaafar ◽  
Taha S. Al-Sakka ◽  
Salama M. Aboelenin ◽  
...  

Thirty multiparous lactating Holstein cows with an average live body weight of 642 ± 21 kg and an average daily milk yield of 30.46 ± 0.59 kg were used in this study. Cows with parities of 2 and 4 were used following their peak period, and were divided into three groups, with ten cows in each group. The control group was fed yellow corn grain rations (YCG), while for the 2nd and 3rd groups, 25 and 50% of YCG was replaced with dry sugar beet pulp (DSBP), denoted as DSBP25 and DSBP50, respectively. The contents of dry matter, organic matter, ether extract, nitrogen-free extract, and fiber carbohydrate in the experimental rations tended to decrease; however, crude protein, crude fiber, ash, and fiber fractions tended to increase in the DSBP25 and DSBP50 groups. Only crude fiber digestibility increased (p < 0.05) in the DSBP rations. Rumen pH value and concentration of ammonia nitrogen (NH3-N) decreased, while the concentration of total volatile fatty acids (TVFAs) increased in the DSBP25 and DSBP50 groups. The concentrations of total protein and globulin in blood plasma were higher (p < 0.05) in DSBP25 and DSBP50 than in YCG. However, plasma albumin concentration, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities were lower (p < 0.05) in DSBP50 than in YCG. Milk yield and yield of 4% fat-corrected milk (4% FCM) were higher (p < 0.05) in DSBP25 and DSBP50 than in YCG. Fat, protein, solids not fat (SNF), and total solids (TS) contents in milk increased significantly (p < 0.05) for feeding rations containing DSBP. Feed cost was reduced, but the output of milk yield increased with DSBP. In conclusion, introducing DSBP into the rations of Holstein dairy cows led to significant improvements in their productive performance.


2018 ◽  
Vol 10 (3) ◽  
pp. 162
Author(s):  
Hayam M. A. Abo El-Maaty ◽  
Sara Kh. Sherif ◽  
Lina S. A. Foda

The current study was designed to evaluate the effect feeding weaning rabbits sugar beet tops (SBT) with prebiotic on digestibility of nutrients and caecal microbial activity, volatile fatty acids estimation and organs histology. Fifty four 6-week-old unsexed weaning NZW rabbits were randomly distributed in a factorial (3 × 2) design to six equal groups. Sugar beet tops (SBT) was used at levels 0.0, 20 or 30% of the diet instead of the percent of alfalfa hay in the control diet in presence of 1.0 g/kg diet or absence of prebiotic (Perfect). Digestibility coefficients of dry matter (DM), crude protein (CP), ether extract (EE) and NFE of 12-week old NZW rabbits were improved by feeding SBT-containing diets. Dietary SBT level had no significant effect on pH value, TVFA,s and NH3-N values for caecum content of 12 weeks old NZW rabbits, however, total bacteria, E. coli and lactobacillus counts were significantly affected. Feeding SBT-containing diets did not alter plasma concentrations of AST and ALT of 12-week-old rabbits. Dietary prebiotic had a positive effect on the digestibility coefficients of DM, OM, CP, EE, CF and NFE of growing rabbits. Added prebiotic had a positive effect on caecal fermentative activities and caecal microbial activity of growing rabbits. The pH value, ammonia-N level, total bacteria and lactobacillus counts of rabbits fed prebiotic-supplemented diets were significantly higher but E. coli count was reduced as compared to control ones. This study concluded that sugar beet tops can be safely used in rabbit diets up to 30% instead of alfalfa hay.


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