scholarly journals Survival of Generic Escherichia coli and Salmonella in Oregon's Agricultural Soils

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Emch Alexander W ◽  
Mohamed Hussein MH ◽  
Cusic Joy G Waite
Keyword(s):  
Escherichia Coli ◽  
Agricultural Soils

Dynamics of Escherichia coli in agricultural soils receiving swine manure slurry or liquid municipal biosolids

2006 ◽  
Vol 86 (5) ◽  
pp. 841-849 ◽  
Author(s):  
Andrew Scott ◽  
Ken Conn ◽  
George Lazarovits ◽  
Edward Topp
Keyword(s):  
Escherichia Coli ◽  
Field Experiments ◽  
Agricultural Soils ◽  
Swine Manure ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Loam Soil ◽  
Municipal Biosolids ◽  
Manure Slurry

The fate of two E. coli stains, one that grows in soil receiving swine manure slurry (SMS) (Strain C279) and one that does not (Strain C278) were evaluated in laboratory and field experiments. A sensitive (detection limit 5 cells g soil-1) microplate-format most probable number (MPN) method for enumerating E. coli was developed and validated. In laboratory incubations, there was a general relationship between manure volatile fatty acid (VFA) content and the ability of the SMS to support growth in soil. Strain C279 grew in SMS-amended loam or sandy soil, but not in silt loam soil. Swine manure slurry inoculated with both Strains C278 and C279 was surface or subsurface applied in field microplots. The population of E. coli increased 50-fold within 3 d, and the ratio of Strain C279 to Strain C278 increased as much as 20-fold during the experiment, consistent with the growth of Strain C279 under these circumstances. Liquid municipal biosolids (LMB) obtained from four cities did not support the growth of Strain C279. The dynamics of E. coli populations in soils receiving surface and subsurface (10 cm) applications of SMS were comparable. Key words: Escherichia coli, water quality, liquid municipal biosolids, swine manure slurry

scholarly journals Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dharmender K. Gahlot ◽  
Nayyer Taheri ◽  
Dhani Ram Mahato ◽  
Matthew S. Francis
Keyword(s):  
Escherichia Coli ◽  
Biological System ◽  
Copper Complexes ◽  
Agricultural Soils ◽  
In Silico Analysis ◽  
Md Simulations ◽  
Integrated Approach ◽  
Industrial Wastes ◽  
Molar Ratio ◽  
Divalent Metal Ions

AbstractHeavy metal sequestration from industrial wastes and agricultural soils is a long-standing challenge. This is more critical for copper since copper pollution is hazardous both for the environment and for human health. In this study, we applied an integrated approach of Darwin’s theory of natural selection with bacterial genetic engineering to generate a biological system with an application for the accumulation of Cu2+ ions. A library of recombinant non-pathogenic Escherichia coli strains was engineered to express seven potential Cu2+ binding peptides encoded by a ‘synthetic degenerate’ DNA motif and fused to Maltose Binding Protein (MBP). Most of these peptide-MBP chimeras conferred tolerance to high concentrations of copper sulphate, and in certain cases in the order of 160-fold higher than the recognised EC50 toxic levels of copper in soils. UV–Vis spectroscopic analysis indicated a molar ratio of peptide-copper complexes, while a combination of bioinformatics-based structure modelling, Cu2+ ion docking, and MD simulations of peptide-MBP chimeras corroborated the extent of Cu2+ binding among the peptides. Further, in silico analysis predicted the peptides possessed binding affinity toward a broad range of divalent metal ions. Thus, we report on an efficient, cost-effective, and environment-friendly prototype biological system that is potentially capable of copper bioaccumulation, and which could easily be adapted for the removal of other hazardous heavy metals or the bio-mining of rare metals.

scholarly journals Survival of Enterohemorrhagic Escherichia coli O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on rpoS and Environmental Factors

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1443
Author(s):  
Katharina Detert ◽  
Herbert Schmidt
Keyword(s):  
Escherichia Coli ◽  
Agricultural Soils ◽  
Pathogen Transmission ◽  
Enterohemorrhagic Escherichia Coli ◽  
Cattle Manure ◽  
E Coli ◽  
Minor Influence ◽  
Two Temperatures ◽  
Crop Harvest ◽  
A Minor

The consumption of contaminated fresh produce caused outbreaks of enterohemorrhagic (EHEC) Escherichia coli. Agricultural soil might be a reservoir for EHEC strains and represent a contamination source for edible plants. Furthermore, the application of manure as fertilizer is an important contamination route. Thus, the German fertilizer ordinance prohibits the use of manure 12 weeks before crop harvest to avoid pathogen transmission into the food chain. In this study, the survival of E. coli O104:H4 strain C227/11Φcu in soil microenvironments with either diluvial sand or alluvial loam at two temperatures was investigated for more than 12 weeks. It was analyzed whether the addition of cattle manure extends EHEC survival in these microenvironments. The experiments were additionally performed with isogenic ΔrpoS and ΔfliC deletion mutants of C227/11Φcu. The survival of C227/11Φcu was highest at 4 °C, whereas the soil type had a minor influence. The addition of cattle manure increased the survival at 22 °C. Deletion of rpoS significantly decreased the survival period under all cultivation conditions, whereas fliC deletion did not have any influence. The results of our study demonstrate that EHEC C227/11Φcu is able to survive for more than 12 weeks in soil microenvironments and that RpoS is an important determinant for survival.

scholarly journals Persistence of Pathogenic and Non-Pathogenic Escherichia coli Strains in Various Tropical Agricultural Soils of India

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130038 ◽  
Author(s):  
S. Naganandhini ◽  
Z. John Kennedy ◽  
M. Uyttendaele ◽  
D. Balachandar
Keyword(s):  
Escherichia Coli ◽  
Agricultural Soils ◽  
Pathogenic Escherichia Coli

Sorption of Escherichia coli in Agricultural Soils Influenced by Swine Manure Constituents

2012 ◽  
Vol 55 (1) ◽  
pp. 61-71 ◽  
Author(s):  
J. A. Guzman ◽  
G. A. Fox ◽  
C. J. Penn
Keyword(s):  
Escherichia Coli ◽  
Agricultural Soils ◽  
Swine Manure

Persistence of Escherichia coli O157 and non-O157 strains in agricultural soils

2014 ◽  
Vol 490 ◽  
pp. 822-829 ◽  
Author(s):  
Jincai Ma ◽  
A. Mark Ibekwe ◽  
David E. Crowley ◽  
Ching-Hong Yang
Keyword(s):  
Escherichia Coli ◽  
Agricultural Soils ◽  
Escherichia Coli O157

Fate of Escherichia coli O157: H7 in agricultural soils amended with different organic fertilizers

2015 ◽  
Vol 296 ◽  
pp. 30-36 ◽  
Author(s):  
Zhiyuan Yao ◽  
Li Yang ◽  
Haizhen Wang ◽  
Jianjun Wu ◽  
Jianming Xu
Keyword(s):  
Escherichia Coli ◽  
Agricultural Soils ◽  
Organic Fertilizers ◽  
Escherichia Coli O157
Sign in / Sign up

Export Citation Format

Share Document