Investigation of Anti-Cancer Nano Drugs' Effects' Trend on Human Pancreas Cancer Cells and Tissues Prevention, Diagnosis and Treatment Process under Synchrotron and X-Ray Radiations with the Passage of Time Using Mathematica

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Heidari Alireza
Keyword(s):  
Cancer Cells ◽  
Treatment Process ◽  
Pancreas Cancer ◽  
Diagnosis And Treatment ◽  
Human Pancreas ◽  
X Ray ◽  
Anti Cancer

Synergistic Cytotoxicity and Molecular Interaction on Drug Targets of Sorafenib and Gemcitabine in Human Pancreas Cancer Cells

Chemotherapy ◽  
2010 ◽  
Vol 56 (4) ◽  
pp. 303-312 ◽  
Author(s):  
Simona Ricciardi ◽  
Valentina Mey ◽  
Sara Nannizzi ◽  
Giuseppe Pasqualetti ◽  
Francesco Crea ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Molecular Interaction ◽  
Drug Targets ◽  
Pancreas Cancer ◽  
Human Pancreas ◽  
Synergistic Cytotoxicity

scholarly journals Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hassan Noorbazargan ◽  
Sobhan Amintehrani ◽  
Aghigh Dolatabadi ◽  
Ainaz Mashayekhi ◽  
Nazanin Khayam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Lung Cancer ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
X Ray ◽  
Juniperus Chinensis ◽  
Anti Cancer

AbstractThe current study evaluated the anti-cancer properties of bio-functionalized silver nanoparticles fabricated by Juniperus chinensis leaf extracts. The nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, dynamic light scattering, Zeta potential and X-ray spectroscopy. Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. The size of the spherical nanoparticle was 12.96 nm with negative zeta potential. Up-regulation of caspase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, and ROS production indicated the remarkable apoptotic effect of AgNPs compared to cisplatin. Moreover, down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs. Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line.

Cytotoxic and Anti-cancer Effects of Nickel Nanowires against Pancreatic Cancer Cells

2012 ◽  
Vol 1416 ◽  
Author(s):  
Md. Zakir Hossain ◽  
Wisam J. Khudhayer ◽  
Rozina Akter ◽  
Tansel Karabacak ◽  
Maurice G. Kleve
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
X Ray ◽  
Pancreatic Cancer Cells ◽  
Nickel Nanowires ◽  
Custom Made ◽  
Anti Cancer ◽  
Contrast Microscopy

ABSTRACTCytotoxicity study of magnetic nanomaterials is a key consideration for biomedical applications. Very little is known about the cytotoxic and anti-cancer effects of nickel nanowires (Ni NWs) on mammalian cells and their interaction with proliferating cancer cells. Current therapeutics do not address the full heterogeneity of pancreatic cancers due to the resistance to apoptosis and does not suffice for a successful treatment. Therefore, synthesis of novel anticancer drugs continues to be a potential topic for pancreatic cancer research. In this study, we have investigated the cellular toxicity and anti-cancer effects of Ni NWs in one of the most aggressive human pancreatic ductal cancer (Panc-1) cell lines with the objective of development of a potential treatment strategy. Ni NWs were fabricated in a custom-made setup utilizing the electrodeposition method. Elemental analysis, crystallographic structure, and morphological properties of the synthesized Ni NWs were investigated using Energy Dispersive X-ray Analysis (EDAX), X-Ray Diffraction (X-RD) and Scanning Electron Microscopy (SEM), respectively. Panc-1 cell cultures were maintained according to a slightly modified American Type Culture Collection (ATCC) protocol. Morphological apoptogenic characteristics assessment of the Ni NWs induced Panc-1 cell was accomplished using phase contrast microscopy (PCM). Two commercially available cytotoxicity procedures including 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and trypan blue (TB) assays were utilized to determine the qualitative and quantitative cytotoxicity and anti-cancer effects of Ni NWs. As a negative control, Panc-1 cells without Ni NWs treatment were used in all experiments. Phase contrast microscopy (PCM) was used to confirm the Ni NWs internalization by Panc-1 cells. Both the MTT and TB assays, qualitatively and quantitatively confirmed the cytotoxic and anti-cancer effects of Ni NWs treated Panc-1 cells in vitro in both concentration and exposure-time dependent manners. We studied the cytotoxic and anti-cancer effects of Ni NWs on Panc-1 cells using novel integrated bionanotechnological approaches to understand the corresponding biological pathway with the objective of developing pancreatic cancer treatment. More specifically, we explored the molecular mechanisms associated with the pathway involved in Ni NWs induced toxicity against Panc-1 cells. Our results demonstrated that Ni NWs show strong candidacy for targeting cell selective applications in pancreatic cancer therapy. Key words: Nickel Nanowires, anti-cancer effects, pancreatic cancer.

scholarly journals Anti-cancer, Anti-invasiveness & Anti-metastasis of Green Engineered Bioorganic-Capped Silver Nanoparticles Fabricated from Juniperus chinensis Extract and Comparison to Cisplatin in Lung Cancer Cells (A549): In Vitro Assessment of Cellular and Molecular Pathways

2020 ◽  
Author(s):  
Hassan Noorbazargan ◽  
Ainaz Mashayekhi ◽  
Nazanin Khayam ◽  
Mohammad Naghizadeh ◽  
Amir Mirzaie ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Lung Cancer ◽  
Zeta Potential ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Lower Side ◽  
X Ray ◽  
Juniperus Chinensis ◽  
Anti Cancer ◽  
Apoptotic Effect

Abstract The current study reveals anti-cancer properties of bio-functionalized silver nanoparticle (AgNPs) fabricated by Juniperus chinensis leaf extracts due to it’s easy, low cost, biological activity, eco-friendly and lower side effects. The characteristics of AgNPs were determined by scanning electron microscopy [1], transmission electron microscopy (TEM), UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, Dynamic light scattering (DLS), Zeta potential and X-ray spectroscopy (EDX). Further, this study highlights the cellular and molecular mechanisms of AgNPs involves in anti-proliferative and apoptotic effect on human lung cancer cells (A549) and compared to commercial drug cisplatin by various biological methods such as MTT, flow cytometery analysis, gene expression patterns, migration and invasion inhibition, ROS and caspase production and cell staining. The size of AgNPs fabricated in this study was 12.96 with spherical shape and negative zeta potential. Up-regulation of capase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, ROS production indicated remarkable apoptotic effect of AgNPs than cisplatin. Also down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs.cell cycle analysis and down regulation of cyclin D1 showed cancer cell cessation in the G0/G1 phase. Overall results in current experiment revealed AgNPs synthetized by biogreen method anti-metastasis and anti-proliferation effect on lung cancer cells comparison to cicplatin drug and also had lower side effect on normal cell line.

scholarly journals Synthesis, Characterization, Anti-Cancer Analysis of Sr0.5Ba0.5DyxSmxFe8−2xO19 (0.00 ≤ x ≤ 1.0) Microsphere Nanocomposites

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 700
Author(s):  
Suhailah S. Al-Jameel ◽  
Munirah A. Almessiere ◽  
Firdos A. Khan ◽  
Nedaa Taskhandi ◽  
Yassine Slimani ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cancer Cells ◽  
Spherical Shape ◽  
Hexagonal Structure ◽  
Dapi Staining ◽  
X Ray ◽  
Transmission Electron ◽  
Anti Cancer ◽  
Hct 116

There is enormous interest in combining two or more nanoparticles for various biomedical applications, especially in anti-cancer agent delivery. In this study, the microsphere nanoparticles were prepared (MSNPs) and their impact on cancer cells was examined. The MSNPs were prepared by using the hydrothermal method where strontium (Sr), barium (Ba), dysprosium (Dy), samarium (Sm), and iron oxide (Fe8−2xO19) were combined, and dysprosium (Dy) and samarium (Sm) was substituted with strontium (Sr) and barium (Ba), preparing Sr0.5Ba0.5DyxSmxFe8−2xO19 (0.00 ≤ x ≤ 1.0) MSNPs. The microspheres were characterized by X-ray powder diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) techniques. The diffraction pattern of nanohexaferrites (NHFs) reflected the signature peaks of the hexagonal structure. The XRD revealed a pure hexagonal structure without any undesired phase, which indicated the homogeneity of the products. The crystal size of the nanoparticles were in the range of 22 to 36 nm by Scherrer’s equation. The SEM of MSNPs showed a semi-spherical shape with a high degree of aggregation. TEM and HR-TEM images of MSNPs verified the spherical shape morphology and structure that approved an M-type hexaferrite formation. The anti-cancer activity was examined on HCT-116 (human colorectal carcinoma) and HeLa (cervical cancer cells) using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and post-48 h treatment of MSNPs caused a dose-dependent inhibition of HCT-116 and HeLa cell proliferation and growth. Conversely, no significant cytotoxic effect was observed on HEK-293 cells. The treatments of MSNPs also induced cancer cells DNA disintegration, as revealed by 4′,6-diamidino-2-phenylindole (DAPI) staining. Finally, these findings suggest that synthesized MSNPs possess potential inhibitory actions on cancerous cells without harming normal cells.

Role of exosomes in diagnosis and therapy of prostate cancer

2020 ◽  
Vol 28 (3) ◽  
pp. 399-405
Author(s):  
Fabrizio Fontana ◽  
Olga A. Babenko
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Biological Fluids ◽  
Diagnosis And Treatment ◽  
Diagnosis And Therapy ◽  
The Future ◽  
Important Direction ◽  
Potential Biomarkers

Aim of this letter is to attract the attention of journal readers to the study of exosomes as an important direction in the development of Oncology, in particular, in the diagnosis and treatment of prostate cancer. Exosomes are produced by tumor cells and regulate proliferation, metastasis, and the development of chemoresistance. Their extraction from biological fluids allows further use of these vesicles as potential biomarkers of prostate cancer. In the future, exosomes can be successfully used in the delivery of drugs and other anti-tumor substances to cancer cells.

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