THE EFFECTS OF L-ORNITINE AND L-ARGININE ON THE PROCESSES OF LIPID PEROXIDATION IN THE FUNCTIONAL LAYERS OF KIDNEYS ON THE BACKGROUND OF ACUTE TOXIC HEPATITIS

2020 ◽  
Vol 73 (11) ◽  
pp. 2498-2502
Author(s):  
Olha M. Krekhovska-Lepiavko ◽  
Borys A. Lokay ◽  
Arsen A. Hudyma ◽  
Svitlana O. Yastremska ◽  
Oksana M. Yurchyshyn ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Carbon Tetrachloride ◽  
Normal Saline ◽  
Renal Cortex ◽  
Toxic Hepatitis ◽  
Renal Medulla ◽  
Renal Papilla ◽  
Renal Cells ◽  
Carbon Tetrachloride Intoxication ◽  
Acute Toxic Hepatitis

The aim is to evaluate the effects of L-arginine and L-ornithine on the processes of lipid peroxidation in homogenates of renal cortex, renal medulla and renal papilla under conditions of acute toxic hepatitis. Materials and methods: The study was performed on 40 outbred white male rats with experimental hepatitis, caused by carbon tetrachloride. The animals were divided into five groups: control group (the rats were simulated carbon tetrachloride poisoning and its correction by administering of olive oil and normal saline in equivalent doses), acute carbon tetrachloride hepatitis (single intraperitoneal injection of 50% carbon tetrachloride oil solution at the dose of 2 mlxkg-1 of body weight and simulation of treatment by administration of normal saline in equivalent doses), acute carbon tetrachloride hepatitis + L-ornithine (1000 mgxkg-1), acute carbon tetrachloride hepatitis + L-arginine (500 mgxkg-1) and acute carbon tetrachloride hepatitis + combination of substances. Results: On the background of acute carbon tetrachloride intoxication it was observed the development of renal failure in experimental animals, manifested by activation of lipid peroxidation processes in homogenates of renal cortex, renal medulla and renal papilla. The administration of L-ornithine and L-arginine demonstrates positive impact on renal function and hepato-renal syndrome by stabilization of cell membranes and regeneration of functional capacity of injured renal cells. Conclusions: The results of our study confirm both the presence of unidirectional effects and absence of toxic influences of L-ornithine and L-arginine on renal cells under the conditions of acute carbon tetrachloride intoxication, which are the most important requirements for modern drugs for the treatment of hepato-renal syndrome.

scholarly journals EVALUATION OF ANTIOXIDANT PROPERTIES OF MEDGERM IN ACUTE SIMULATED HEPATITIS

2020 ◽  
Vol 20 (1) ◽  
pp. 59-66
Author(s):  
O. L. Tymchishin ◽  
V.V. Godovan ◽  
N.V. Kresyun
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant System ◽  
Liver Tissue ◽  
Toxic Hepatitis ◽  
Antioxidant Properties ◽  
Male Rats ◽  
Group I ◽  
Acute Toxic Hepatitis ◽  
Main Components ◽  
Acute Toxic

Disruption in redox processes plays a significant role in the pathogenesis of hepatitis. A balance between peroxidation, on the one hand, and the antioxidant system, on the other, is a prerequisite for maintaining normal cell activity. The modern arsenal of effective hepatoprotective drugs is not so wide therefore, the development of new hepatoprotectors is one of the topical issues in pharmacology. The purpose of the work is study the antioxidant properties of medgerma in acute experimental hepatitis. Material and methods. The experiments were performed on male rats weighing 180-220 g. Acute toxic hepatitis in rats was induced by intraperitoneal administration of D-galactosamine in a dosage of 400 mg / kg (LD50). The experimental animals were divided into 4 groups: group I (n = 10) included intact animals that were injected 0.9% sodium chloride solution (control group) intraperitoneally, II group (n = 40) involved the animals receiving only D-galactosamine; group III (n = 40) included rats, which received were administered Medmerg intraperitoneally in a dose of 0.4 mg / kg 7 days before D-galactosamine administration and 7 days after D-galactosamine administration. Group IV (n = 40) included rats, which received the comparison medicine, Essentiale® N, in at a dosage of 5 mg / kg in the same mode. The assessment of lipid peroxidation indices and the antioxidant system indices were carried out on days 1, 3, and 7 after the administration of hepatotoxin in the blood serum and the liver tissue homogenate supernatant. The condition of lipid peroxidation processes in the animals with galactosamine hepatitis was assessed by the content of lipid peroxidation end products in the blood and liver tissue that react with 2-thiobarbituric acid (TBA reactants). The condition of the main components of the antioxidant system was evaluated by the level of reduced glutathione and by the activity of catalase and superoxide dismutase. Results and discussion. It has been established that medgerm enhances the liver resistance to hepatotoxicant, as evidenced by a decrease in the level of lipid peroxidation products and the consumption of main components of the antioxidant system on the first day of hepatitis. Moreover, in the rats, which received Medgerm according to prevention scheme under acute toxic hepatitis, the course of the disease was milder than in the animals that did not receive this therapy. Conclusions and prospects for further research. Medgerm possesses hepatoprotective properties, which underlay positive effects on the state of lipid peroxidation and the main components of the antioxidant system.

The effect of the drug "Hepaton" on the reaction of lipid peroxidation

2020 ◽  
Vol 2 ◽  
pp. 112-115
Author(s):  
V.S. Ponamarev ◽  
◽  
O.S. Popova ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Toxic Hepatitis ◽  
Antioxidant Properties ◽  
The Body ◽  
Intragastric Administration ◽  
Bile Formation ◽  
Laboratory Rats ◽  
Acute Toxic Hepatitis ◽  
Acute Toxic

The composition of the drug "Hepaton" includes many bioactive compounds that provide the antioxidant properties of the drug, manifested by the neutralization of reactive oxygen species (ROS) with the breakaway chain free radical reactions. The aim of the study was to determine the effect of the Hepaton preparation on lipid peroxidation reactions in laboratory rats. Evaluation of the antioxidant effect of the drug was carried out on twenty laboratory rats of both sexes with a body weight of 180-220 g, divided into 2 groups. Acute toxic hepatitis in rats was induced by a single intragastric administration of 1.0 ml of a dichloroethane solution. At the same time, rats of the experimental group (n = 10) 1 hour before the introduction of di-chloroethane were injected with a solution of the drug “Hepaton” in the amount of 10 ml / kg body weight and then 1 time per day for 21 days after the use of toxicants [1]. On the 21st day after the administration of toxicants, a blood was drawn for a biochemi-cal study, which took into account the pa-rameters of the antioxidant system (diene conjugates (DC), ketodienes (CD) and malondialdehyde (MDA), as well as the level of endogenous intoxication (according to the content of MSM). Based on the results obtained, it can be con-cluded that the use of “Hepaton” in modeling acute toxic hepatitis made it possible to re-store the disturbed homeostasis of the labora-tory animal organism, the structure and in-tegrity of the hepatocyte membranes, inhibit lipid peroxidation as one of the links in the pathogenesis of hepatitis, stimulate antioxi-dant defense and power the endogenous anti-oxidant system of the body, bile formation and biliary excretion, as well as activate the reparative processes of the liver tissue at the cellular and intracellular levels.

scholarly journals THE INFLUENCE OF COPTISINE BISULFATE ON THE EVOLUTION OF ACUTE TOXIC HEPATITIS

World Science ◽  
2016 ◽  
Vol 2 (8(36)) ◽  
pp. 42-46
Author(s):  
Peredelcu Rodica ◽  
Gonciar Veaceslav ◽  
Scutari Corina ◽  
Cazacu Vasile
Keyword(s):  
Alkaline Phosphatase ◽  
Lactate Dehydrogenase ◽  
Carbon Tetrachloride ◽  
Toxic Hepatitis ◽  
Albumin Level ◽  
Chelidonium Majus ◽  
White Rats ◽  
Acute Toxic Hepatitis ◽  
Hepatic Cytolysis ◽  
Acute Toxic

In the experiments made on white rats was studied the influence of coptisine bisulfate, alkaloid extracted from Chelidonium majus, in the following doses: 10 mg/kg and 20 mg/kg on acute toxic hepatitis cause by carbon tetrachloride. It was established that the researched substance reduced hepatic cytolysis and cholestasis through reestablishment of the transaminases activity and lactate dehydrogenase, while lowering the alkaline phosphatase/alanine aminotransferase ratio and modulated the deflection of the metabolic parameters of acute toxic hepatitis. Coptisine bisulfate corrected the carbon tetrachloride caused hypoproteinemia when administered for 7 days and normalized the albumin level at 14th day of treatment of acute toxic hepatitis.

5′-Methylthioadenosine administration prevents lipid peroxidation and fibrogenesis induced in rat liver by carbon-tetrachloride intoxication

2001 ◽  
Vol 34 (3) ◽  
pp. 386-394 ◽  
Author(s):  
Maria M Simile ◽  
Sebastiano Banni ◽  
Elisabetta Angioni ◽  
Gianfranca Carta ◽  
Maria R De Miglio ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Carbon Tetrachloride ◽  
Rat Liver ◽  
Carbon Tetrachloride Intoxication

scholarly journals IMMUNE AND METABOLIC DISTURBANCES IN EXPERIMENTAL ACUTE TOXIC HEPATITIS: CORRECTION BY XENOGENIC AND ALLOGENIC HEPATOCYTES

2016 ◽  
Vol 18 (2) ◽  
pp. 91-98 ◽  
Author(s):  
A. I. Konoplya ◽  
E. S. Litvinova ◽  
N. A. Bystrova ◽  
M. S. Razumova ◽  
T. V. Chuyeva
Keyword(s):  
Carbon Tetrachloride ◽  
Metabolic Activity ◽  
Toxic Hepatitis ◽  
Liver Toxicity ◽  
Effective Means ◽  
Free Radical Oxidation ◽  
Metabolic Disturbances ◽  
Acute Toxic Hepatitis ◽  
The Impact ◽  
Acute Toxic

For correspondence: Konoplya Alexander Ivanovich. Address: 3,K. Marx St.,Kursk, 305041,Russian Federation. Tel.: job. (4712) 58-81-76; mob. (910) 317-87-88. E-mail: [email protected]. To study the corrective effects of allogeneic and xenogeneic hepatocytes on metabolic disturbances in acute liver toxicity.Material and methods. Investigations were carried out on 75 adult male Wistar rats weighing 120–160 g, 15 rats and 25 mice on the 5–6th days after birth. Acute toxic hepatitis (ATH) was modeled by intramuscular injection of carbon tetrachloride at a dose of 3 ml / kg as a 50% solution in olive oil, five times at 24-hour intervals. Isolating xenogeneic (mouse) and allogeneic hepatocytes was performed by method of Berry M.N., Friend D.S. The cell suspension was prepared daily and administered at a concentration of 2 × 106 /kg in recipients with ATH intraperitoneally, five times at 24-hour intervals, simultaneously with the first injection of hepatotropic poison.Results. Intoxication by carbon tetrachloride causes development of the biochemical syndromes of liver damage, activation of the functional metabolic activity of peripheral blood neutrophils and free-radical oxidation, breaks intraerythrocytic metabolism. The introduction of allogeneic hepatocytes in recipients with toxic hepatopathy is more efficiently compared with xenogeneic hepatocytes, it corrects local and systemic metabolic disturbances arising due to the impact of hepatotropic poison. Conclusion. Transplantation of xenogenic hepatocytes, and, to a greater extent, of allogenic hepatocytes in ATH conditions is an effective means to restore the functional metabolic activity of hepatocytes, neutrophils and erythrocytes. 

scholarly journals Hepatoprotective activity of a new germanium-organic biologically active substance (medgerm) in experimental hepatitis

2013 ◽  
Vol 94 (5) ◽  
pp. 628-632 ◽  
Author(s):  
O L Tymchyshin
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant System ◽  
Active Substance ◽  
Toxic Hepatitis ◽  
Biologically Active ◽  
Male Rats ◽  
Biologically Active Substance ◽  
Drug Determination ◽  
Acute Toxic Hepatitis ◽  
Acute Toxic

Aim. To study of the influence of new germanium-organic biologically active substance (medgerm) on lipid peroxidation and antioxidant system in rats with galactosamin-induced acute hepatitis. Methods. The experiments were carried out on Wistar male rats. Acute toxic hepatitis in rats was induced by intraperitoneal injection of D-galactosamine. Experimental animals were divided into 4 groups: first group (n=10) - intact animals that received 0.9% sodium chloride solution intraperitoneal injections throughout the observation period (control group); second group (n=40) - animals that received only D-galactosamine; third group (n=40) - rats that received medgerm intraperitoneal injections 7 days before and 7 days after the administration of D-galactosamine. The dose and administration regimen of medgerm were pre-defined. The fourth group (n=40) included rats that received Essentiale® N in the same mode as a comparator drug. Determination of thiobarbituric acid reactants, reduced glutathione levels in serum and liver homogenate supernatant, as well as superoxide dismutase and catalase activities were carried out in 1, 3, 5 and 7 days after the administration of liver toxins. Results. Medgerm has a significant effect on pro- and antioxidant homeostasis in acute toxic galactosamine-induced hepatitis. It effectively prevented the generation and accumulation of lipid peroxidation end products and preserved the activity of nonenzymatic and enzymatic antioxidant system parts. In animals treated with medgerm, studied parameters restored to reach the baseline level faster than in rats with galactosamine-induced hepatitis that were not treated with medgerm. Conclusion. The findings suggest that medgerm has the antioxidant activity and a membrane-mediated action in acute toxic hepatitis.

Sign in / Sign up

Export Citation Format

Share Document