scholarly journals STRUCTURAL REORGANIZATION OF THE RATS’ SUBMANDIBULAR GLANDS ACINI AFTER THE INFLUENCE OF 1% METHACRYLATE

2020 ◽  
Vol 73 (7) ◽  
pp. 1318-1322
Author(s):  
Galyna А. Yeroshenko Yeroshenko ◽  
Larysa Ya. Fedoniuk ◽  
Konstantyn V. Shevchenko ◽  
Denys R. Kramarenko ◽  
Аnastasiia І. Yachmin ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Normal Condition ◽  
Polymeric Materials ◽  
Morphometric Study ◽  
Secretory Cells ◽  
Control Group ◽  
Outer Diameter ◽  
Submandibular Glands ◽  
White Rats ◽  
Submandibular Salivary Glands

Introduction: Polymeric substances, which are used in medicine, can lead to the development of pathological conditions. Therefore, in toxicological evaluation of polymeric materials for medical purposes the primary task is the creation of the appropriate adequate methods of study. The aim: To study the acini of the rats’ submandibular salivary glands in normal condition and after exposure of 1% methacrylate. Material and methods: Were studied 50 white rats during the influence of 1% methacrylate on the mucosa of the oral cavity. Histological sections of the rats’ submandibular salivary glands in normal condition, on day 14 and 30 of experiment were studied using the UMPT – 7 ultramicrotome of Sumy PA “Selmi”. Results and conclusions: The morphometric study has established that in control group the values of the outer diameter of the submandibular glands’ acini, the diameter of the lumen and the height of the acini epithelial cells was 36,27±2,17 μm, 9,47±0,63 μm and 14,18±1,05 μm, respectively. Histological specimens showed that the acini cells had a prominent basophilia of the cytoplasm and the basally located nucleus. Numerous large homogeneous granules were located in the apical pole of the cells. After influence of the 1% methacrylat there is an increase in the activity of secretory cells, which is confirmed by an increase in the height of epitheliocytes by 25.04% and is a compensatory metacrylate reaction. The increase in salivation further leads to complete depletion of the secretory apparatus of the seromucosal cells, which is confirmed by a decrease in the outer and inner diameters with a decrease in the height of the mandibular salivary glands acini cells by 24.40%.

scholarly journals REACTIVE CHANGES IN THE RESISTIVE OF THE HEMOMYCOCIRCULATORY BED FOLLOWING THE ACTION OF 1% OF METHACRYLIC ACID ESTER

2019 ◽  
Vol 19 (2) ◽  
pp. 118-122
Author(s):  
D. R. Kramarenko ◽  
K. V. Shevchenko ◽  
A. I. Yachmin
Keyword(s):  
Salivary Gland ◽  
Methyl Ester ◽  
Methacrylic Acid ◽  
Acid Methyl Ester ◽  
Vascular Wall ◽  
Morphometric Study ◽  
Control Group ◽  
Submandibular Salivary Gland ◽  
Outer Diameter ◽  
Acid Methyl

The morphometric study conducted has revealed that the effect of a 1% methacrylic acid-methyl ester solution affects the resistive section of the microvasculatory bed of the submandibular salivary gland lobes. The study was conducted on 30 white outbred male rats, 10 of which made up the control group of animals and 20 animals made up experimental group, whose oral mucosa was treated with 1% methacrylic acid-methyl ester solution for 30 days. After euthanasia of animals in 14 and 30 days, samples of the submandibular gland were embedded into epon-812. Semi-thin sections were stained with polychrome dye. A quantitative analysis of the results of the morphometric study and statistical processing of the morphometric data was performed using standard statistical methods using the Excel program. The outer diameter and lumen diameter of arterioles were measured. The thickness of the vascular wall was calculated using the formula VWTh = Dz-Dt / 2. On the 14th day, in response to the action of 1% methacrylic acid-methyl ester solution, the average outer arteriole diameter decreased by 13.04%, the diameter of the of arteriole lumen by 20.77%. There is a significant increase in the average thickness of the vascular wall by 5.45%, the value of which was 2.90 microns. On the 13th day of the study, it was found out that the average values ​​of the outer diameter of the arterioles increased by 5.04% (p <0.05), compared with the control group. The lumen diameter was significantly larger by 15% that the values ​​in the control group of animals, and the average vascular wall thickness was significantly lower by 18.91% than the values ​​in the control group of animals (p <0.05). Normalization of indicators for the 30th day has not been defined. Thus, the effect of 1% methacrylic acid-methyl ester solution on the arterioles of the submandibular salivary gland lobes is characterized by vascular spasms in the early stages, and vascular dilatation in the later stages of the experiment.

Increase in weight of the submandibular salivary glands of rats following periodic amputation of the erupted portion of the incisor teeth

1959 ◽  
Vol 196 (4) ◽  
pp. 827-830 ◽  
Author(s):  
Herbert Wells ◽  
S. Jerome Zackin ◽  
Paul Gold-Haber ◽  
Paul L. Munson
Keyword(s):  
Salivary Glands ◽  
Chronic Treatment ◽  
Dry Weight ◽  
Albino Rats ◽  
Submandibular Glands ◽  
Submandibular Salivary Glands ◽  
Starting Point ◽  
Lower Incisors

Periodic amputation of the erupted portion of the lower incisors of albino rats resulted in a marked increase in the wet and dry weight of the submandibular salivary glands as early as 7 days after the first amputation. Enlargement of both serous and mucous acini was observed, but no effect on the cells of the tubules could be detected. Amputation of the upper incisors alone did not result in enlargement of the submandibular glands. Hypophysectomy decreased the extent of the response to amputation of the lower incisors but did not abolish it. Chronic treatment with cortisone had no significant effect on the weight of the glands. A reflex neurologic explanation was offered as a tentative basis for understanding the phenomenon and as a starting point for further investigation.

scholarly journals Гістологічна та морфометрична характеристики великих слинних залоз в щурів у нормі

2015 ◽  
Author(s):  
В. В Магльона
Keyword(s):  
Salivary Glands ◽  
Quantitative Characteristic ◽  
Histological Structure ◽  
Morphometric Characteristics ◽  
Parotid Glands ◽  
Connective Tissue Layer ◽  
Submandibular Glands ◽  
Submandibular Salivary Glands ◽  
Histological Data ◽  
Better Than

ГІСТОЛОГІЧНА ТА МОРФОМЕТРИЧНА ХАРАКТЕРИСТИКИ ВЕЛИКИХ СЛИННИХ ЗАЛОЗ В ЩУРІВ У НОРМІ - Метою дослідження було встановити особливості гістологічної будови і морфометричну характеристику структур великих слинних залоз в щурів у нормі. Особливістю привушних залоз є, зокрема, те, що вони збудовані переважно з білкових ацинусів, разом з тим, як у нижньощелепних залозах ацинуси мають змішаний білково-слизовий тип і продукують як білковий, так і слизовий секрет. Ще однією із відмінностей слинних залоз щурів різної локалізації є те, що у нижньощелепних залозах значно краще, ніж у привушних, виражені сполучнотканинні прошарки між частками і сполучнотканинні муфти навкола проток і судин. Отримані гістологічні дані й морфометричні показники можуть складати основу для порівняння при експериментальному моделюванні різноманітних патологічних процесів та кількісної характеристики патоморфологічнихзмін, що виникають при цьому.<br />ГИСТОЛОГИЧЕСКАЯ И МОРФОМЕТРИЧЕСКАЯ ХАРАКТЕРИСТИКИ БОЛЬШИХ СЛЮННЫХ ЖЕЛЕЗ У КРЫС В НОРМЕ - Целью исследования было установление особенностей гистологического строения и морфометрическая характеристика структур больших слюнных желез у крыс в норме. Особенностью околоушных желез является, в частности, то, что они построены в основном из белковых ацинусов, в то время, как в нижнечелюстных железах ацинусы имеют смешанный белково-слизистый тип и продуцируют как белковый, так и слизистый секрет. Еще одной из отличительных особенностей слюнных желез крыс различной локализации является то, что в нижнечелюстных железах значительно лучше, чем в околоушных выражены соединительнотканные прослойки между дольками и соединительнотканные муфты вокруг протоков и сосудов. Полученные гистологические данные и морфометрические показатели могут составлять основу для сравнения при экспериментальном моделировании различных патологических процессов и количественной характеристики патоморфологических изменений, возникающих при этом.<br />HISTOLOGICAL AND MORPHOMETRIC CHARACTERISTICS OF THE MAJOR SALIVARY GLANDS OF RATS IN NORM - The aim of the study was to establish the features of histological structure and morphometric characteristics of rats large salivary glands structures in norm. Particulary the feature of parotid glands is that they are constructed mainly of protein acini, while the acini of submandibular glands are mixed - mucous and protein type which produce both protein and mucous secretion. Another distinctive feature of different localization rats salivary glands is that connective tissue layer between the lobules and connective couplings around the ducts and blood vessels in the submandibular glands are expressed better than in the parotid. Finded histological data and morphometric parameters can form the basis for comparison with experimental modeling of various pathological processes and quantitative characteristic of the pathological changes that occur at that time.<br />Ключові слова: привушна, нижньощелепна слинна залоза, структура, морфометрія.<br />Ключевые слова: околоушная, нижнечелюстная слюнная железа, структура, морфометрия.<br />Key words: parotid, submandibular salivary glands, structure, morphometry.

scholarly journals Attenuating Effect of Vitamin E against Silver Nano Particles Toxicity in Submandibular Salivary Glands

2021 ◽  
Vol 8 (12) ◽  
pp. 219
Author(s):  
Mahmoud M. Bakr ◽  
Mahmoud M. Al-Ankily ◽  
Sara M. Shogaa ◽  
Mohamed Shamel
Keyword(s):  
Vitamin E ◽  
Salivary Glands ◽  
Antioxidant Properties ◽  
Antimicrobial Properties ◽  
Nano Particles ◽  
Control Group ◽  
Albino Rats ◽  
Immune Reactivity ◽  
Immunohistochemical Markers ◽  
Submandibular Salivary Glands

Silver nanoparticles (AgNPs) are extensively used in many industries due to their superior antimicrobial properties. However, it is evident from many studies that AgNPs has cytotoxic potential through its effect on excessive formation of reactive oxygen species (ROS). The aim of this study was to examine the toxic effect of AgNPs on the submandibular salivary glands and the attenuating effect of vitamin E, as a natural antioxidant, against this toxicity. Thirty Albino rats were divided into 3 groups (n = 10): control group, AgNPs group receiving 2 mg/kg daily for 28 days, and AgNPs and vitamin E group receiving AgNPs the same as the previous group in addition to vitamin E at a dose of 100 mg/kg. Microscopic, ultrastructural, and cytokeratin immune-reactivity examination of the glands were performed. The AgNPs group showed noticeable degeneration in all structures of the gland as evident in the histological and ultrastructural examination. The AgNPs and vitamin E group revealed an improvement of the glandular elements. A significant increase in cytokeratin immune expression was found after comparison of both groups (p = 0.01). This current study shows that vitamin E has powerful antioxidant properties, which can combat the cytotoxic effect caused by AgNPs. Further studies are deemed necessary to confirm this finding using other immunohistochemical markers, such as myosin and E-cadherin.

scholarly journals Morphofunctional reorg anisation of intraorg an vessels of rat’s large salivary glands in the experimental diabetes mellitus

2016 ◽  
Author(s):  
I. M. Yavorska-Skrabut
Keyword(s):  
Diabetes Mellitus ◽  
Salivary Glands ◽  
Experimental Diabetes ◽  
Experimental Diabetes Mellitus ◽  
Small Arteries ◽  
Adaptive Changes ◽  
Submandibular Glands ◽  
Vascular Lumen ◽  
White Rats ◽  
Further Development

There was studied the morphofunctional reorganization of the intraorgan vessels of parotid and submandibular glands of white rats with experimental hyperglycemia duration of 1, 2 and 3 months. There was established the development of hyperglycemia and presence of adaptive changes in early terms of observation that appears with constriction of small arteries while expanding of the larger caliber vascular lumen. With increasing duration of hyperglycemia, progressing of vasoconstriction and further development and deepening of destructive and sclerotic  changes in vessels of parotid and submandibular glands were established.

scholarly journals MORPHOMETRIC DESCRIPTION OF THE EXCHANGE SEGMENT OF MICROVASCULATURE OF RATS’ SALIVARY GLANDS IN NORMAL CONDITIONS AND CHRONIC ETHANOL INTOXICATION

2019 ◽  
Vol 72 (3) ◽  
pp. 323-326 ◽  
Author(s):  
Kostyantyn V. Shevchenko ◽  
Galyna A. Yeroshenko ◽  
Оlena S. Yakushko ◽  
Kateryna S. Kazakova ◽  
Denys R. Kramarenko
Keyword(s):  
Standard Procedure ◽  
Early Observation ◽  
Chronic Ethanol ◽  
Control Group ◽  
Albino Rats ◽  
Outer Diameter ◽  
Ethanol Intoxication ◽  
Submandibular Glands ◽  
Intact Group ◽  
Chronic Ethanol Intoxication

Introduction: The state of the segments of the microvasculature, especially exchange one, has a significant effect on the organs’ functions. The results of it’s research can be objectified by morphometric method. The aim: to determine the dynamics of changes in metric indices of the exchange segment of microvasculature of rats’ submandibular glands in normal conditions and in chronic ethanol intoxication. Materials and methods: 45 albino rats were involved into study. Intact group (n=5) animals were administered with NaCl isotonic solution 4 times a day directly into the stomach. Experimental group (n=40) animals were administered with 40° ethanol 4 times a day directly into the stomach. Animals were sacrificed on 5, 9, 12 and 30 days by overdose of thiopental anesthesia. Lobules of submandibular glands were embedded into epon-812 according to standard procedure. Results: On day 5 of the experiment the outer diameter of the capillary wall significantly reduced (4,91±0,02 μm), that is 19,5 % less than the values in controls (р<0,05). On day 9 of the experiment it was 15,2 % lower than the value of control group (р>0,05). On day 30 of the experiment the outer capillary diameter was 8 % lower compared to controls (р<0,05). Conclusions: The early observation showed vasodilatation, confirmed by the constriction of the outer diameter and lumen diameter, accompanied by the thickening of the vascular wall under the influence of chronic ethanol intoxication. The indices did not come to normal values by the 30-th day of the experiment.

scholarly journals Morphometric study of angioarchitectonic under the effect of opioid (experimental study)

2020 ◽  
Vol 26 (3) ◽  
pp. 31-36
Author(s):  
L.R. Mateshuk-Vatseba ◽  
U.Ye. Pidvalna ◽  
A.M. Bekesevych
Keyword(s):  
Experimental Study ◽  
Cerebellar Cortex ◽  
Tissue Expansion ◽  
Morphometric Study ◽  
Control Group ◽  
Triggering Mechanism ◽  
Narcotic Analgesics ◽  
Professional Literature ◽  
White Rats ◽  
Trophic Activity

Opioids are potent drugs that are widely used to combat pain in severe wounds and in cancer patients. The professional literature provides a limited amount of data on the morphometric analysis of the links of the hemomicrocirculatory bed of the cerebellar cortex and choroid of the eyeball under the influence of an opioid in the experiment. The aim of the work was to establish the features and conduct morphometric studies of the links of the hemomicrocirculatory bed of the cerebellar cortex and choroid in normal conditions and under conditions of 6-week exposure to an opioid in the experiment. The experimental study was carried out on 24 male white rats, 3 months old and weighing 160-180 g. The material for the study is represented by preparations of the choroid and cerebellum with an injected vascular bed. Compared with the control group of animals, profound destructive changes in the angioarchitectonics of the choroid and cerebellar cortex were found under the influence of Nalbuphine. The capillary component is destroyed, the arterioles are tortuous, sclerosed, their lumen is uneven, the wall is thickened, the venules are dilated and deformed. A decrease in comparison with the norm in the diameter of arterioles, the density of the network of exchange vessels, arterio-venular coefficient, as well as an increase in the diameter of venules and an indicator of trophic activity of the tissue. Expansion of arterio-venular anastomoses indicates the discharge of blood from the arterioles into the venous bed, bypassing the destroyed capillaries. A pronounced relationship was established between the depth of structural transformations of the hemomicrocirculatory bed of the cerebellar cortex, choroid of the white rat’s eye and morphometric parameters. It has been confirmed that the triggering mechanism of destructive changes under the influence of narcotic analgesics is the development of angiopathy.

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